|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45776||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Modifications to backbonepTacI promoter was removed and replaced by PLtetO-1. Untranslated region was removed and replaced by UTR1, a powerful UTR. Luc gene (firefly Luciferase) was removed and replaced by LacI-YbaQ. A transcriptional terminator was added, called T500.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameLac repressor
Insert Size (bp)1080
- Promoter PLtetO-1
/ Fusion Protein
- YbaQ degradation tag
- Cloning method Unknown
- 5′ sequencing primer CATGGTGAAGACTATCGCAC
- 3′ sequencing primer GAAGGAGCTGACTGGGTTGA (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byVincent Noireaux, University of Minnesota
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBEST-PLtetO-1-UTR1-LacI-YbaQ-T500 was a gift from Richard Murray & Vincent Noireaux (Addgene plasmid # 45776 ; http://n2t.net/addgene:45776 ; RRID:Addgene_45776)