pCM132
(Plasmid
#45829)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45829 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCM66
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Backbone manufacturerLidstrom Lab (Addgene plasmid 45827)
- Backbone size (bp) 11299
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Modifications to backboneCreated by inserting the blunted 3.2 kb EcoRI fragment from pLacZ2.1+ containing lacZ into the blunted BamHI site of pCM66 to create pCM66LacZ. The introduction of trrnB was accomplished by inserting the 1.0 kb EcoRI-RsrII fragment of pCM130 into pCM66LacZ cut with EcoRI and RsrII.
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Vector typelow-background broad-host-range promoter-probe vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
LacZ inserted into pCM66 (Addgene plasmid 45827) in opposite orientation to lac promoter and is used to test promoter activity. Transcriptional terminator added to reduce background.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM132 was a gift from Mary Lidstrom (Addgene plasmid # 45829) -
For your References section:
Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Marx CJ, Lidstrom ME. Microbiology. 2001 Aug;147(Pt 8):2065-75. 10.1099/00221287-147-8-2065 PubMed 11495985