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pCM132
(Plasmid #45829)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45829 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCM66
  • Backbone manufacturer
    Lidstrom Lab (Addgene plasmid 45827)
  • Backbone size (bp) 11299
  • Modifications to backbone
    Created by inserting the blunted 3.2 kb EcoRI fragment from pLacZ2.1+ containing lacZ into the blunted BamHI site of pCM66 to create pCM66LacZ. The introduction of trrnB was accomplished by inserting the 1.0 kb EcoRI-RsrII fragment of pCM130 into pCM66LacZ cut with EcoRI and RsrII.
  • Vector type
    low-background broad-host-range promoter-probe vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LacZ inserted into pCM66 (Addgene plasmid 45827) in opposite orientation to lac promoter and is used to test promoter activity. Transcriptional terminator added to reduce background.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM132 was a gift from Mary Lidstrom (Addgene plasmid # 45829)
  • For your References section:

    Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Marx CJ, Lidstrom ME. Microbiology. 2001 Aug;147(Pt 8):2065-75. 10.1099/00221287-147-8-2065 PubMed 11495985