Addgene - pRS314-4M5.3 Plasmid Data

Plasmid 45830: pRS314-4M5.3

Gene/insert name:
4M5.3
Insert size:
801
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Vector backbone:
pRS314
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
6190
Promoter:
GAL1
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CTGGGGTAATTAATCAGCGAAGCG List of Sequencing Primers
3' sequencing primer:
GTACGAGCTAAAAGTACAGTGGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
TRP1
Person or lab that originally
cloned the gene/insert:
Plasmid pRS314 from PMID:2659436
Sequence: View sequences (4)
Principal Investigator:
Dane Wittrup
Terms and Licenses:
UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 45830" in your Materials and Methods section.