Addgene - pRS314-4M5.3 Plasmid Data

Plasmid 45830: pRS314-4M5.3

Gene/insert name:
4M5.3
Insert size:
801
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Vector backbone:
pRS314
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
6190
Promoter:
GAL1
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CTGGGGTAATTAATCAGCGAAGCG List of Sequencing Primers
3' sequencing primer:
GTACGAGCTAAAAGTACAGTGGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
TRP1
Person or lab that originally
cloned the gene/insert:
Plasmid pRS314 from PMID:2659436
Sequence: View sequences (4)
Principal Investigator:
Dane Wittrup
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 45830" in your Materials and Methods section.