pRS314-4M5.3
(Plasmid
#45830)
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Depositing Lab
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Publication
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Sequence Information
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Depositor Sequences: Full (1)
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Addgene Sequences: Partial (3)
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 45830 | Plasmid sent as bacteria in agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS314
- Backbone size w/o insert (bp) 6190
- Total vector size (bp) 6991
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name4M5.3
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Insert Size (bp)801
- Promoter GAL1
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CTGGGGTAATTAATCAGCGAAGCG
- 3′ sequencing primer GTACGAGCTAAAAGTACAGTGGG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPlasmid pRS314 from PMID:2659436
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Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS314-4M5.3 was a gift from Dane Wittrup (Addgene plasmid # 45830)