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pGR-ECK120033127
(Plasmid #46011)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46011 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGR
  • Backbone size w/o insert (bp) 5047
  • Total vector size (bp) 5092
  • Modifications to backbone
    Inserted ECK120033127 terminator between the EcoRI and SpeI sites.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ECK120033127 Terminator
  • Species
    E. coli
  • Insert Size (bp)
    45
  • Promoter PBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer gtccacacaatctgcccttt
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGR-ECK120033127 was a gift from Christopher Voigt (Addgene plasmid # 46011 ; http://n2t.net/addgene:46011 ; RRID:Addgene_46011)
  • For your References section:

    Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt CA. Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515. 10.1038/nmeth.2515 PubMed 23727987