TroponinT-Gateway
(Plasmid
#46026)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46026 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneFUdeltaGW-rtTA
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Modifications to backboneUbC-rtTA excised and replaced with the human TNNT2 promoter and the Gateway cloning cassette from pEF-DEST51.
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Vector typeLentiviral
- Promoter TNNT2
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 3473, 3937, 4087 & 4822 when compared to the full plasmid sequence. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TroponinT-Gateway was a gift from John Gearhart (Addgene plasmid # 46026 ; http://n2t.net/addgene:46026 ; RRID:Addgene_46026) -
For your References section:
Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, Christoforou N, Epstein JA, Gearhart JD. J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004. 10.1016/j.yjmcc.2013.04.004 PubMed 23591016
