pENTR-NICD1
(Plasmid #46048)

• Depositing Lab: Xin Chen
• Publication: Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5. doi: 10.1172/JCI63212. Epub 2012 Jul 17.

Backbone

• Vector backbone: pENTR
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2700
• Vector type: Entry Vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Notch1 (C-term)
• Alt name: Notch 1
• Species: M. musculus (mouse)
• Insert Size (bp): 2385
• Mutation: Cytoplasmic domain initiating at amino acid 1753
• GenBank ID: NM_008714.3
• Entrez Gene: Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
• Tag / Fusion Protein:
  • Myc tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: pENTR-Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Notch 1 from Addgene plasmid 15131: pLIA mNIC myc.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry