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Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
(Plasmid #46168)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46168 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2641
  • Total vector size (bp) 7799
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    codon optimized Cas9_SV40 NLS with intron
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    5158
  • Mutation
    intron inserted at position 3113-3163, and SV40 NLS inserted from position 5192-5227
  • Promoter eef-1A.1 (eft-3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13 (-40) forward
  • 3′ sequencing primer M13 (-48) reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For more information on Calarco Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/calarco/

Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Peft-3::cas9-SV40_NLS::tbb-2 3'UTR was a gift from John Calarco (Addgene plasmid # 46168 ; http://n2t.net/addgene:46168 ; RRID:Addgene_46168)
  • For your References section:

    Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco JA. Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532. 10.1038/nmeth.2532 PubMed 23817069