pET15b/GgVcl 1-1066 (full length)
(Plasmid #46171)

• Depositing Lab: Susan Craig
• Publication: Bakolitsa et al Nature. 2004 Jul 29;430(6999):583-6. Epub 2004 Jun 13.

Backbone

• Vector backbone: pET-15b
• Backbone manufacturer: EMD
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 8909
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vcl
• Alt name: Vinculin
• Species: G. gallus (chicken)
• Insert Size (bp): 3200
• GenBank ID: NM_205441.1
• Entrez Gene: VCL (a.k.a. VINC1)
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry