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pET15b/GgVcl 1-1066 (full length)
(Plasmid #46171)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46171 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    EMD
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 8909
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vcl
  • Alt name
    Vinculin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    3200
  • GenBank ID
    NM_205441.1
  • Entrez Gene
    VCL
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b/GgVcl 1-1066 (full length) was a gift from Susan Craig (Addgene plasmid # 46171 ; http://n2t.net/addgene:46171 ; RRID:Addgene_46171)
  • For your References section:

    Structural basis for vinculin activation at sites of cell adhesion. Bakolitsa C, Cohen DM, Bankston LA, Bobkov AA, Cadwell GW, Jennings L, Critchley DR, Craig SW, Liddington RC. Nature. 2004 Jul 29;430(6999):583-6. Epub 2004 Jun 13. 10.1038/nature02610 PubMed 15195105