mcherry(C1)_RepoMan
(Plasmid #46274)

• Depositing Labs: Angus Lamond, Laura Trinkle-Mulcahy
• Publication: Trinkle-Mulcahy et al J Cell Biol. 2006 Feb 27;172(5):679-92. Epub 2006 Feb 21.

Backbone

• Vector backbone: mcherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7772
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RepoMan
• Alt name: CDCA2
• Species: H. sapiens (human)
• Insert Size (bp): 3072
• GenBank ID: NM_152562
• Entrez Gene: CDCA2 (a.k.a. PPP1R81, Repo-Man)
• Promoter: CMV
• Tag / Fusion Protein:
  • mcherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Asp718 (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: CAACGTCAACATCAAGTTGG
• 3′ sequencing primer: GTTTGGACAAACCACAACTAGAATGCAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry