GgVcl 1-1066 FRET tail probe 2
(Plasmid #46282)

• Depositing Lab: Susan Craig
• Publication: Chen et al J Biol Chem. 2006 Dec 29;281(52):40389-98. Epub 2006 Oct 29.

Backbone

• Vector backbone: pECFP-N3
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 8659
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Vcl 1-1066 + mCitrine
• Alt name: Vinculin
• Alt name: Vcl
• Species: G. gallus (chicken)
• Mutation: mCitrine (and linker) inserted after aa883
• Entrez Gene: VCL (a.k.a. VINC1)
• Promoter: CMV
• Tag / Fusion Protein:
  • ECFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: ?

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Similar to FRET probe 1 (Addgene plasmid 46276) except ECFP changed to monomeric Citrine.