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GgVcl 1-1066 FRET tail probe 2
(Plasmid #46282)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46282 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pECFP-N3
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 8659
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vcl 1-1066 + mCitrine
  • Alt name
    Vinculin
  • Alt name
    Vcl
  • Species
    G. gallus (chicken)
  • Mutation
    mCitrine (and linker) inserted after aa883
  • Entrez Gene
    VCL
  • Promoter CMV
  • Tag / Fusion Protein
    • ECFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer ?
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Similar to FRET probe 1 (Addgene plasmid 46276) except ECFP changed to monomeric Citrine.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GgVcl 1-1066 FRET tail probe 2 was a gift from Susan Craig (Addgene plasmid # 46282 ; http://n2t.net/addgene:46282 ; RRID:Addgene_46282)
  • For your References section:

    Coincidence of actin filaments and talin is required to activate vinculin. Chen H, Choudhury DM, Craig SW. J Biol Chem. 2006 Dec 29;281(52):40389-98. Epub 2006 Oct 29. 10.1074/jbc.M607324200 PubMed 17074767