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pT3TS-nCas9n Citations (57)

Plasmid Article: Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system.
Jao LE, Wente SR, Chen W Proc Natl Acad Sci U S A. 2013 Aug 5.
PubMed Journal

Articles Citing pT3TS-nCas9n

Articles
Targeted mutagenesis in Atlantic salmon (Salmo salar L.) using the CRISPR/Cas9 system induces complete knockout individuals in the F0 generation. Edvardsen RB, Leininger S, Kleppe L, Skaftnesmo KO, Wargelius A. PLoS One. 2014 Sep 25;9(9):e108622. doi: 10.1371/journal.pone.0108622. eCollection 2014. PubMed
High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis. Thomas HR, Percival SM, Yoder BK, Parant JM. PLoS One. 2014 Dec 11;9(12):e114632. doi: 10.1371/journal.pone.0114632. eCollection 2014. PubMed
Rapid reverse genetic screening using CRISPR in zebrafish. Shah AN, Davey CF, Whitebirch AC, Miller AC, Moens CB. Nat Methods. 2015 Jun;12(6):535-40. doi: 10.1038/nmeth.3360. Epub 2015 Apr 13. PubMed
CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity. Carrington B, Varshney GK, Burgess SM, Sood R. Nucleic Acids Res. 2015 Dec 15;43(22):e157. doi: 10.1093/nar/gkv802. Epub 2015 Aug 7. PubMed
The Autotaxin-Lysophosphatidic Acid Axis Modulates Histone Acetylation and Gene Expression during Oligodendrocyte Differentiation. Wheeler NA, Lister JA, Fuss B. J Neurosci. 2015 Aug 12;35(32):11399-414. doi: 10.1523/JNEUROSCI.0345-15.2015. PubMed
Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System. Yin L, Jao LE, Chen W. Methods Mol Biol. 2015;1332:205-17. doi: 10.1007/978-1-4939-2917-7_16. PubMed
Accumulation of the Vitamin D Precursor Cholecalciferol Antagonizes Hedgehog Signaling to Impair Hemogenic Endothelium Formation. Cortes M, Liu SY, Kwan W, Alexa K, Goessling W, North TE. Stem Cell Reports. 2015 Oct 13;5(4):471-9. doi: 10.1016/j.stemcr.2015.08.010. Epub 2015 Sep 10. PubMed
Targeted candidate gene screens using CRISPR/Cas9 technology. Shah AN, Moens CB, Miller AC. Methods Cell Biol. 2016;135:89-106. doi: 10.1016/bs.mcb.2016.01.008. Epub 2016 Feb 26. PubMed
Optimized CRISPR-Cas9 System for Genome Editing in Zebrafish. Vejnar CE, Moreno-Mateos MA, Cifuentes D, Bazzini AA, Giraldez AJ. Cold Spring Harb Protoc. 2016 Oct 3;2016(10):pdb.prot086850. doi: 10.1101/pdb.prot086850. PubMed
Deep Brain Photoreceptor (val-opsin) Gene Knockout Using CRISPR/Cas Affects Chorion Formation and Embryonic Hatching in the Zebrafish. Hang CY, Moriya S, Ogawa S, Parhar IS. PLoS One. 2016 Oct 28;11(10):e0165535. doi: 10.1371/journal.pone.0165535. eCollection 2016. PubMed
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish. Varshney GK, Carrington B, Pei W, Bishop K, Chen Z, Fan C, Xu L, Jones M, LaFave MC, Ledin J, Sood R, Burgess SM. Nat Protoc. 2016 Dec;11(12):2357-2375. doi: 10.1038/nprot.2016.141. Epub 2016 Oct 27. PubMed
Autolysosome biogenesis and developmental senescence are regulated by both Spns1 and v-ATPase. Sasaki T, Lian S, Khan A, Llop JR, Samuelson AV, Chen W, Klionsky DJ, Kishi S. Autophagy. 2017 Feb;13(2):386-403. doi: 10.1080/15548627.2016.1256934. Epub 2016 Nov 22. PubMed
Mechanical stretch triggers rapid epithelial cell division through Piezo1. Gudipaty SA, Lindblom J, Loftus PD, Redd MJ, Edes K, Davey CF, Krishnegowda V, Rosenblatt J. Nature. 2017 Mar 2;543(7643):118-121. doi: 10.1038/nature21407. Epub 2017 Feb 15. PubMed
Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation. Akerberg AA, Henner A, Stewart S, Stankunas K. Dev Biol. 2017 Jun 1;426(1):84-96. doi: 10.1016/j.ydbio.2017.03.030. Epub 2017 Apr 1. PubMed
Loss of the Habenula Intrinsic Neuromodulator Kisspeptin1 Affects Learning in Larval Zebrafish. Lupton C, Sengupta M, Cheng RK, Chia J, Thirumalai V, Jesuthasan S. eNeuro. 2017 May 19;4(3). pii: ENEURO.0326-16.2017. doi: 10.1523/ENEURO.0326-16.2017. eCollection 2017 May-Jun. PubMed
Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish. Wehner D, Tsarouchas TM, Michael A, Haase C, Weidinger G, Reimer MM, Becker T, Becker CG. Nat Commun. 2017 Jul 25;8(1):126. doi: 10.1038/s41467-017-00143-0. PubMed
Interhost dispersal alters microbiome assembly and can overwhelm host innate immunity in an experimental zebrafish model. Burns AR, Miller E, Agarwal M, Rolig AS, Milligan-Myhre K, Seredick S, Guillemin K, Bohannan BJM. Proc Natl Acad Sci U S A. 2017 Oct 17;114(42):11181-11186. doi: 10.1073/pnas.1702511114. Epub 2017 Oct 2. PubMed
A shared Runx1-bound Zbtb16 enhancer directs innate and innate-like lymphoid lineage development. Mao AP, Ishizuka IE, Kasal DN, Mandal M, Bendelac A. Nat Commun. 2017 Oct 16;8(1):863. doi: 10.1038/s41467-017-00882-0. PubMed
G protein-coupled estrogen receptor regulates embryonic heart rate in zebrafish. Romano SN, Edwards HE, Souder JP, Ryan KJ, Cui X, Gorelick DA. PLoS Genet. 2017 Oct 24;13(10):e1007069. doi: 10.1371/journal.pgen.1007069. eCollection 2017 Oct. PubMed
Alk and Ltk ligands are essential for iridophore development in zebrafish mediated by the receptor tyrosine kinase Ltk. Mo ES, Cheng Q, Reshetnyak AV, Schlessinger J, Nicoli S. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12027-12032. doi: 10.1073/pnas.1710254114. Epub 2017 Oct 23. PubMed
Nuclear Androgen Receptor Regulates Testes Organization and Oocyte Maturation in Zebrafish. Crowder CM, Lassiter CS, Gorelick DA. Endocrinology. 2018 Feb 1;159(2):980-993. doi: 10.1210/en.2017-00617. PubMed
apterous A specifies dorsal wing patterns and sexual traits in butterflies. Prakash A, Monteiro A. Proc Biol Sci. 2018 Feb 28;285(1873). pii: rspb.2017.2685. doi: 10.1098/rspb.2017.2685. PubMed
Cystathionine beta-Synthase Is Necessary for Axis Development in Vivo. Prabhudesai S, Koceja C, Dey A, Eisa-Beygi S, Leigh NR, Bhattacharya R, Mukherjee P, Ramchandran R. Front Cell Dev Biol. 2018 Feb 16;6:14. doi: 10.3389/fcell.2018.00014. eCollection 2018. PubMed
Genetic analysis of zebrafish homologs of human FOXQ1, foxq1a and foxq1b, in innate immune cell development and bacterial host response. Earley AM, Dixon CT, Shiau CE. PLoS One. 2018 Mar 13;13(3):e0194207. doi: 10.1371/journal.pone.0194207. eCollection 2018. PubMed
The Hippo pathway effector Wwtr1 regulates cardiac wall maturation in zebrafish. Lai JKH, Collins MM, Uribe V, Jimenez-Amilburu V, Gunther S, Maischein HM, Stainier DYR. Development. 2018 May 17;145(10). pii: 145/10/dev159210. doi: 10.1242/dev.159210. PubMed
Usherin defects lead to early-onset retinal dysfunction in zebrafish. Dona M, Slijkerman R, Lerner K, Broekman S, Wegner J, Howat T, Peters T, Hetterschijt L, Boon N, de Vrieze E, Sorusch N, Wolfrum U, Kremer H, Neuhauss S, Zang J, Kamermans M, Westerfield M, Phillips J, van Wijk E. Exp Eye Res. 2018 Aug;173:148-159. doi: 10.1016/j.exer.2018.05.015. Epub 2018 May 16. PubMed
Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9. Prykhozhij SV, Fuller C, Steele SL, Veinotte CJ, Razaghi B, Robitaille JM, McMaster CR, Shlien A, Malkin D, Berman JN. Nucleic Acids Res. 2018 Sep 28;46(17):e102. doi: 10.1093/nar/gky512. PubMed
Zebrafish mutants and TEAD reporters reveal essential functions for Yap and Taz in posterior cardinal vein development. Astone M, Lai JKH, Dupont S, Stainier DYR, Argenton F, Vettori A. Sci Rep. 2018 Jul 5;8(1):10189. doi: 10.1038/s41598-018-27657-x. PubMed
A molecular mechanism for Wnt ligand-specific signaling. Eubelen M, Bostaille N, Cabochette P, Gauquier A, Tebabi P, Dumitru AC, Koehler M, Gut P, Alsteens D, Stainier DYR, Garcia-Pino A, Vanhollebeke B. Science. 2018 Aug 17;361(6403). pii: science.aat1178. doi: 10.1126/science.aat1178. Epub 2018 Jul 19. PubMed
Multiplexed CRISPR/Cas9 Targeting of Genes Implicated in Retinal Regeneration and Degeneration. Unal Eroglu A, Mulligan TS, Zhang L, White DT, Sengupta S, Nie C, Lu NY, Qian J, Xu L, Pei W, Burgess SM, Saxena MT, Mumm JS. Front Cell Dev Biol. 2018 Aug 21;6:88. doi: 10.3389/fcell.2018.00088. eCollection 2018. PubMed
Robust activation of microhomology-mediated end joining for precision gene editing applications. Ata H, Ekstrom TL, Martinez-Galvez G, Mann CM, Dvornikov AV, Schaefbauer KJ, Ma AC, Dobbs D, Clark KJ, Ekker SC. PLoS Genet. 2018 Sep 12;14(9):e1007652. doi: 10.1371/journal.pgen.1007652. eCollection 2018 Sep. PubMed
Targeted disruption of the endogenous zebrafish rhodopsin locus as models of rapid rod photoreceptor degeneration. Zelinka CP, Sotolongo-Lopez M, Fadool JM. Mol Vis. 2018 Aug 27;24:587-602. eCollection 2018. PubMed
Poor Splice-Site Recognition in a Humanized Zebrafish Knockin Model for the Recurrent Deep-Intronic c.7595-2144A>G Mutation in USH2A. Slijkerman R, Goloborodko A, Broekman S, de Vrieze E, Hetterschijt L, Peters T, Gerits M, Kremer H, van Wijk E. Zebrafish. 2018 Dec;15(6):597-609. doi: 10.1089/zeb.2018.1613. Epub 2018 Oct 3. PubMed
Color opponency with a single kind of bistable opsin in the zebrafish pineal organ. Wada S, Shen B, Kawano-Yamashita E, Nagata T, Hibi M, Tamotsu S, Koyanagi M, Terakita A. Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):11310-11315. doi: 10.1073/pnas.1802592115. Epub 2018 Oct 15. PubMed
Precise A*T to G*C base editing in the zebrafish genome. Qin W, Lu X, Liu Y, Bai H, Li S, Lin S. BMC Biol. 2018 Nov 20;16(1):139. doi: 10.1186/s12915-018-0609-1. PubMed
The mineralocorticoid receptor is essential for stress axis regulation in zebrafish larvae. Faught E, Vijayan MM. Sci Rep. 2018 Dec 27;8(1):18081. doi: 10.1038/s41598-018-36681-w. PubMed
Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport. Insinna C, Lu Q, Teixeira I, Harned A, Semler EM, Stauffer J, Magidson V, Tiwari A, Kenworthy AK, Narayan K, Westlake CJ. Nat Commun. 2019 Jan 25;10(1):428. doi: 10.1038/s41467-018-08192-9. PubMed
Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization. Murdoch CC, Espenschied ST, Matty MA, Mueller O, Tobin DM, Rawls JF. PLoS Pathog. 2019 Mar 7;15(3):e1007381. doi: 10.1371/journal.ppat.1007381. eCollection 2019 Mar. PubMed

Associated Plasmids

GPCR-independent activation of G proteins promotes apical cell constriction in vivo. Marivin A, Morozova V, Walawalkar I, Leyme A, Kretov DA, Cifuentes D, Dominguez I, Garcia-Marcos M. J Cell Biol. 2019 May 6;218(5):1743-1763. doi: 10.1083/jcb.201811174. Epub 2019 Apr 4. PubMed
Guidelines for optimized gene knockout using CRISPR/Cas9. Campenhout CV, Cabochette P, Veillard AC, Laczik M, Zelisko-Schmidt A, Sabatel C, Dhainaut M, Vanhollebeke B, Gueydan C, Kruys V. Biotechniques. 2019 Jun;66(6):295-302. doi: 10.2144/btn-2018-0187. Epub 2019 May 1. PubMed
Manipulation of Gene Function in Mexican Cavefish. Stahl BA, Jaggard JB, Chin JSR, Kowalko JE, Keene AC, Duboue ER. J Vis Exp. 2019 Apr 22;(146). doi: 10.3791/59093. PubMed
Genome-wide strategies reveal target genes of Npas4l associated with vascular development in zebrafish. Marass M, Beisaw A, Gerri C, Luzzani F, Fukuda N, Gunther S, Kuenne C, Reischauer S, Stainier DYR. Development. 2019 Jun 3;146(11). pii: dev.173427. doi: 10.1242/dev.173427. PubMed
CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon (Salmo salar L.) inhibits elongation of polyunsaturated fatty acids and induces Srebp-1 and target genes. Datsomor AK, Zic N, Li K, Olsen RE, Jin Y, Vik JO, Edvardsen RB, Grammes F, Wargelius A, Winge P. Sci Rep. 2019 May 17;9(1):7533. doi: 10.1038/s41598-019-43862-8. PubMed
CRISPR-Cas9 Mediated Genome Editing in Bicyclus anynana Butterflies. Banerjee TD, Monteiro A. Methods Protoc. 2018 May 15;1(2). pii: mps1020016. doi: 10.3390/mps1020016. PubMed
Agouti-Related Protein 2 Is a New Player in the Teleost Stress Response System. Shainer I, Michel M, Marquart GD, Bhandiwad AA, Zmora N, Ben-Moshe Livne Z, Zohar Y, Hazak A, Mazon Y, Forster D, Hollander-Cohen L, Cone RD, Burgess HA, Gothilf Y. Curr Biol. 2019 Jun 17;29(12):2009-2019.e7. doi: 10.1016/j.cub.2019.05.021. Epub 2019 Jun 6. PubMed
Brd4 and P300 Confer Transcriptional Competency during Zygotic Genome Activation. Chan SH, Tang Y, Miao L, Darwich-Codore H, Vejnar CE, Beaudoin JD, Musaev D, Fernandez JP, Benitez MDJ, Bazzini AA, Moreno-Mateos MA, Giraldez AJ. Dev Cell. 2019 Jun 17;49(6):867-881.e8. doi: 10.1016/j.devcel.2019.05.037. PubMed
TAF1, associated with intellectual disability in humans, is essential for embryogenesis and regulates neurodevelopmental processes in zebrafish. Gudmundsson S, Wilbe M, Filipek-Gorniok B, Molin AM, Ekvall S, Johansson J, Allalou A, Gylje H, Kalscheuer VM, Ledin J, Anneren G, Bondeson ML. Sci Rep. 2019 Jul 24;9(1):10730. doi: 10.1038/s41598-019-46632-8. PubMed
Creation of zebrafish knock-in reporter lines in the nefma gene by Cas9-mediated homologous recombination. Eschstruth A, Schneider-Maunoury S, Giudicelli F. Genesis. 2020 Jan;58(1):e23340. doi: 10.1002/dvg.23340. Epub 2019 Sep 30. PubMed
Loss of rps9 in Zebrafish Leads to p53-Dependent Anemia. Chen C, Huang H, Yan R, Lin S, Qin W. G3 (Bethesda). 2019 Dec 3;9(12):4149-4157. doi: 10.1534/g3.119.400585. PubMed
CRISPR/Cas9-mediated editing of Delta5 and Delta6 desaturases impairs Delta8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.). Datsomor AK, Olsen RE, Zic N, Madaro A, Bones AM, Edvardsen RB, Wargelius A, Winge P. Sci Rep. 2019 Nov 15;9(1):16888. doi: 10.1038/s41598-019-53316-w. PubMed
Expanding the CRISPR Toolbox with ErCas12a in Zebrafish and Human Cells. Wierson WA, Simone BW, WareJoncas Z, Mann C, Welker JM, Kar B, Emch MJ, Friedberg I, Gendron WAC, Barry MA, Clark KJ, Dobbs DL, McGrail MA, Ekker SC, Essner JJ. CRISPR J. 2019 Dec;2(6):417-433. doi: 10.1089/crispr.2019.0026. Epub 2019 Nov 19. PubMed
Clinical and preclinical therapeutic outcome metrics for USH2A-related disease. Toms M, Dubis AM, de Vrieze E, Tracey-White D, Mitsios A, Hayes M, Broekman S, Baxendale S, Utoomprurkporn N, Bamiou D, Bitner-Glindzicz M, Webster AR, Van Wijk E, Moosajee M. Hum Mol Genet. 2020 Jul 21;29(11):1882-1899. doi: 10.1093/hmg/ddaa004. PubMed
Temporal and spatial expression of zebrafish mctp genes and evaluation of frameshift alleles of mctp2b. Espino-Saldana AE, Duran-Rios K, Olivares-Hernandez E, Rodriguez-Ortiz R, Arellano-Carbajal F, Martinez-Torres A. Gene. 2020 May 15;738:144371. doi: 10.1016/j.gene.2020.144371. Epub 2020 Jan 27. PubMed
Genome Editing in Zebrafish Using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task. Prykhozhij SV, Cordeiro-Santanach A, Caceres L, Berman JN. Methods Mol Biol. 2020;2115:385-405. doi: 10.1007/978-1-0716-0290-4_21. PubMed
Impaired mitophagy links mitochondrial disease to epithelial stress in methylmalonyl-CoA mutase deficiency. Luciani A, Schumann A, Berquez M, Chen Z, Nieri D, Failli M, Debaix H, Festa BP, Tokonami N, Raimondi A, Cremonesi A, Carrella D, Forny P, Kolker S, Diomedi Camassei F, Diaz F, Moraes CT, Di Bernardo D, Baumgartner MR, Devuyst O. Nat Commun. 2020 Feb 20;11(1):970. doi: 10.1038/s41467-020-14729-8. PubMed
miR-103/107 regulates left-right asymmetry in zebrafish by modulating Kupffer's vesicle development and ciliogenesis. Heigwer J, Kutzner J, Haeussler M, Burkhalter MD, Draebing T, Juergensen L, Katus HA, Philipp M, Westhoff JH, Hassel D. Biochem Biophys Res Commun. 2020 Jun 25;527(2):432-439. doi: 10.1016/j.bbrc.2020.04.066. Epub 2020 Apr 22. PubMed
Efficient targeted integration directed by short homology in zebrafish and mammalian cells. Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, Carlson DF, Dobbs DL, McGrail M, Essner J. Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968. PubMed

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