pCEV-G2-Ph
(Plasmid
#46816)
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Purpose(Empty Backbone) TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46816 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSP-G2
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Modifications to backboneURA3 gene replaced with ble gene for phleomycin/bleomycin resistance
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Vector typeYeast Expression
- Promoter TEF1 and PGK1
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Selectable markersPhleomycin
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Tag
/ Fusion Protein
- FLAG and c-myc
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSelection in E. coli can be performed on Amp or phleomycin. Selection in yeast can be performed on phleomycin. See publication for details
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer see publication for details
- 3′ sequencing primer see publication for details (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCEV-G2-Ph was a gift from Lars Nielsen & Claudia Vickers (Addgene plasmid # 46816 ; http://n2t.net/addgene:46816 ; RRID:Addgene_46816) -
For your References section:
Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae. Vickers CE, Bydder SF, Zhou Y, Nielsen LK. Microb Cell Fact. 2013 Oct 25;12(1):96. 10.1186/1475-2859-12-96 PubMed 24161108
Map uploaded by the depositor.
