pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense
PurposeExpress cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46839||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 6955
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCoral Green Fluorescent Protein (cGFP)
Insert Size (bp)720
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CGC AAA TGG GCG GTA GGC GTG (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense was a gift from Phil Sharp (Addgene plasmid # 46839)
For your References section:A triple helix stabilizes the 3' ends of long noncoding RNAs that lack poly(A) tails. Wilusz JE, JnBaptiste CK, Lu LY, Kuhn CD, Joshua-Tor L, Sharp PA. Genes Dev. 2012 Nov 1;26(21):2392-407. doi: 10.1101/gad.204438.112. Epub 2012 Oct 16. 10.1101/gad.204438.112 PubMed 23073843
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.