pDL278
(Plasmid #46882)

• Purpose: (Empty Backbone) E. Coli/Gram-positive Bacterial Shuttle Vector
• Depositing Lab: Gary Dunny
• Publication: LeBlanc et al Plasmid. 1992 Sep;28(2):130-45.

Backbone

• Vector backbone: pDL276
• Backbone manufacturer: Addgene plasmid 46881
• Backbone size (bp): 6733
• Modifications to backbone: Kanamycin resistance-encoding aphA3 gene and pVA380-1 ORF-1 of pDL276 replaced with spectinomycin resistance-encoding aad9 gene and pVA380-1 ORF-1 of pDL602 (see associated article for detailed construction information).
• Vector type: Bacterial Expression ; E. coli/Gram-positive Bacterial Shuttle Vector

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: For E. coli, grow in Luria broth (LB) agar containing 50μg/mL Spectinomycin. For E. faecalis, select at 500μg/mL in culture and 1000μg/mL on plates.
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13_pUC_rev
• 3′ sequencing primer: M13_pUC_fwd; pBAD_rev

Resource Information

• Supplemental Documents:
  • pDL278 genbank file
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid may contain unstable elements. Prolonged growth in E. coli selects for deletions, but not in E. faecalis. Test multiple clones upon to ensure the full plasmid is selected.