pET-28b-Cas9-His Citations (25)

Originally described in: Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, Montague TG, Zimmerman S, Richter C, Schier AF PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. PubMed Journal

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Articles Citing pET-28b-Cas9-His

Articles
Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing. Mout R, Ray M, Yesilbag Tonga G, Lee YW, Tay T, Sasaki K, Rotello VM. ACS Nano. 2017 Mar 28;11(3):2452-2458. doi: 10.1021/acsnano.6b07600. Epub 2017 Jan 31. PubMed
A right-handed signalling pathway drives heart looping in vertebrates. Ocana OH, Coskun H, Minguillon C, Murawala P, Tanaka EM, Galceran J, Munoz-Chapuli R, Nieto MA. Nature. 2017 Sep 6;549(7670):86-90. doi: 10.1038/nature23454. PubMed
Comparative analysis of lipid-mediated CRISPR-Cas9 genome editing techniques. Ringer KP, Roth MG, Garey MS, Piorczynski TB, Suli A, Hansen JM, Alder JK. Cell Biol Int. 2018 Jul;42(7):849-858. doi: 10.1002/cbin.10952. Epub 2018 Mar 14. PubMed
A conserved Shh cis-regulatory module highlights a common developmental origin of unpaired and paired fins. Letelier J, de la Calle-Mustienes E, Pieretti J, Naranjo S, Maeso I, Nakamura T, Pascual-Anaya J, Shubin NH, Schneider I, Martinez-Morales JR, Gomez-Skarmeta JL. Nat Genet. 2018 Apr;50(4):504-509. doi: 10.1038/s41588-018-0080-5. Epub 2018 Mar 19. PubMed
Lamellar projections in the endolymphatic sac act as a relief valve to regulate inner ear pressure. Swinburne IA, Mosaliganti KR, Upadhyayula S, Liu TL, Hildebrand DGC, Tsai TY, Chen A, Al-Obeidi E, Fass AK, Malhotra S, Engert F, Lichtman JW, Kirchhausen T, Betzig E, Megason SG. Elife. 2018 Jun 19;7. pii: 37131. doi: 10.7554/eLife.37131. PubMed
DNA-Free Genome Editing of Brassica oleracea and B. rapa Protoplasts Using CRISPR-Cas9 Ribonucleoprotein Complexes. Murovec J, Gucek K, Bohanec B, Avbelj M, Jerala R. Front Plant Sci. 2018 Nov 5;9:1594. doi: 10.3389/fpls.2018.01594. eCollection 2018. PubMed
Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, Aryee MJ, Joung JK. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. PubMed Associated Plasmids pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848) pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815) pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776) pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491) pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495) More ...
Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology. Warner BK, Alder JK, Suli A. Methods Mol Biol. 2019;1965:235-250. doi: 10.1007/978-1-4939-9182-2_16. PubMed
On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains. Carmignotto GP, Azzoni AR. J Biotechnol. 2019 Dec 20;306:62-70. doi: 10.1016/j.jbiotec.2019.09.012. Epub 2019 Sep 20. PubMed
Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Reuven N, Adler J, Broennimann K, Myers N, Shaul Y. Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. PubMed Associated Plasmids pU6-(BsaI)_CBh-Cas9 pU6-(BsaI)_CBh-UN-Cas9 pU6-(BsaI)_CBh-Cas9-T2A-mCherry pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry
An efficient gene knock-in strategy using 5'-modified double-stranded DNA donors with short homology arms. Yu Y, Guo Y, Tian Q, Lan Y, Yeh H, Zhang M, Tasan I, Jain S, Zhao H. Nat Chem Biol. 2020 Apr;16(4):387-390. doi: 10.1038/s41589-019-0432-1. Epub 2019 Dec 23. PubMed
Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA. Saha C, Mohanraju P, Stubbs A, Dugar G, Hoogstrate Y, Kremers GJ, van Cappellen WA, Horst-Kreft D, Laffeber C, Lebbink JHG, Bruens S, Gaskin D, Beerens D, Klunder M, Joosten R, Demmers JAA, van Gent D, Mouton JW, van der Spek PJ, van der Oost J, van Baarlen P, Louwen R. Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi: 10.1126/sciadv.aaz4849. eCollection 2020 Jun. PubMed Associated Plasmids pCjeCas9 pCjedCas9
Chimeric DNA-RNA Guide RNA Designs. Lu S, Zhang Y, Yin H. Methods Mol Biol. 2021;2162:79-85. doi: 10.1007/978-1-0716-0687-2_6. PubMed
Development of aptamer-based inhibitors for CRISPR/Cas system. Zhao J, Inomata R, Kato Y, Miyagishi M. Nucleic Acids Res. 2021 Feb 22;49(3):1330-1344. doi: 10.1093/nar/gkaa865. PubMed
Vasohibin 1 selectively regulates secondary sprouting and lymphangiogenesis in the zebrafish trunk. de Oliveira MB, Meier K, Jung S, Bartels-Klein E, Coxam B, Geudens I, Szymborska A, Skoczylas R, Fechner I, Koltowska K, Gerhardt H. Development. 2021 Feb 19;148(4). pii: dev.194993. doi: 10.1242/dev.194993. PubMed
Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic orco mutation in honey bees. Chen Z, Traniello IM, Rana S, Cash-Ahmed AC, Sankey AL, Yang C, Robinson GE. J Neurogenet. 2021 Sep;35(3):320-332. doi: 10.1080/01677063.2021.1887173. Epub 2021 Mar 5. PubMed
Chlamydomonas POLQ is necessary for CRISPR/Cas9-mediated gene targeting. Sizova I, Kelterborn S, Verbenko V, Kateriya S, Hegemann P. G3 (Bethesda). 2021 Apr 9. pii: 6219301. doi: 10.1093/g3journal/jkab114. PubMed
CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells. Ghetti S, Burigotto M, Mattivi A, Magnani G, Casini A, Bianchi A, Cereseto A, Fava LL. STAR Protoc. 2021 Mar 24;2(2):100407. doi: 10.1016/j.xpro.2021.100407. eCollection 2021 Jun 18. PubMed
CRISPR ribonucleoprotein-mediated genetic engineering in plants. Zhang Y, Iaffaldano B, Qi Y. Plant Commun. 2021 Feb 10;2(2):100168. doi: 10.1016/j.xplc.2021.100168. eCollection 2021 Mar 8. PubMed
The Shh/Gli3 gene regulatory network precedes the origin of paired fins and reveals the deep homology between distal fins and digits. Letelier J, Naranjo S, Sospedra-Arrufat I, Martinez-Morales JR, Lopez-Rios J, Shubin N, Gomez-Skarmeta JL. Proc Natl Acad Sci U S A. 2021 Nov 16;118(46). pii: 2100575118. doi: 10.1073/pnas.2100575118. PubMed
Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing. Lainscek D, Forstneric V, Mikolic V, Malensek S, Pecan P, Bencina M, Sever M, Podgornik H, Jerala R. Nat Commun. 2022 Jun 23;13(1):3604. doi: 10.1038/s41467-022-31386-1. PubMed
DNA-functionalized colloidal crystals for macromolecular encapsulation. Yokomori M, Suzuki H, Nakamura A, Sugano SS, Tagawa M. Soft Matter. 2022 Sep 21;18(36):6954-6964. doi: 10.1039/d2sm00949h. PubMed
Gating and ion selectivity of Channelrhodopsins are critical for photo-activated orientation of Chlamydomonas as shown by in vivo point mutation. Baidukova O, Oppermann J, Kelterborn S, Fernandez Lahore RG, Schumacher D, Evers H, Kamrani YY, Hegemann P. Nat Commun. 2022 Nov 25;13(1):7253. doi: 10.1038/s41467-022-35018-6. PubMed
Site-specific gene knock-in and bacterial phytase gene expression in Chlamydomonas reinhardtii via Cas9 RNP-mediated HDR. Zadabbas Shahabadi H, Akbarzadeh A, Ofoghi H, Kadkhodaei S. Front Plant Sci. 2023 May 19;14:1150436. doi: 10.3389/fpls.2023.1150436. eCollection 2023. PubMed
Enhancing CRISPR prime editing by reducing misfolded pegRNA interactions. Zhang W, Petri K, Ma J, Lee H, Tsai CL, Joung JK, Yeh JJ. bioRxiv [Preprint]. 2023 Aug 15:2023.08.14.553324. doi: 10.1101/2023.08.14.553324. PubMed

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