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pcDNA3.1-GoldenGate
(Plasmid #47388)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47388 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 6546
  • Vector type
    Mammalian Expression, TALEN
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • SV40 NLS (N terminal on insert)
    • HA (C terminal on insert)
    • SV40 NLS (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-GoldenGate was a gift from Charles Gersbach (Addgene plasmid # 47388)
  • For your References section:

    Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak F, Crawford GE, Hartemink AJ, Gersbach CA. Nat Methods. 2013 Mar;10(3):239-42. doi: 10.1038/nmeth.2361. Epub 2013 Feb 3. 10.1038/nmeth.2361 PubMed 23377379