pEN_TT 3xHA-MKK6-EE
(Plasmid #47544)

• Purpose: Gateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutant
• Depositing Lab: Kevin Janes
• Publication: Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-76. doi: 10.1016/j.chom.2012.11.009. Epub 2013 Jan 16.

Backbone

• Vector backbone: pEN_TTmiRc2
• Backbone manufacturer: Addgene #2752
• Backbone size w/o insert (bp): 4422
• Vector type: Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 3xHA-MKK6-EE
• Alt name: MKK6
• Alt name: PRMK6
• Alt name: MEK6
• Species: H. sapiens (human)
• Insert Size (bp): 1095
• Mutation: S207E, T211E
• GenBank ID: NM_002758
• Entrez Gene: MAP2K6 (a.k.a. MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3)
• Tag / Fusion Protein:
  • 3XHA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: MfeI (not destroyed)
• 5′ sequencing primer: pCEP-fwd
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene #13518

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry