Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pGEX-4T-1 3xFlag-ERK2
(Plasmid #47573)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47573 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5053
  • Total vector size (bp) 6130
  • Modifications to backbone
    3xFlag tag inserted at BamHI site of pGEX-4T-1, regenerating the BamHI site downstream of 3xFlag, with a unique HindIII site added between the BamHI and EcoRI sites of pGEX-4T-1.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ERK2
  • Alt name
    MAPK1
  • Alt name
    p42-MAPK
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1077
  • GenBank ID
    NM_053842
  • Entrez Gene
    Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • 3xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #8974.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1 3xFlag-ERK2 was a gift from Kevin Janes (Addgene plasmid # 47573 ; http://n2t.net/addgene:47573 ; RRID:Addgene_47573)
  • For your References section:

    A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. Bose AK, Janes KA. Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 10.1074/mcp.O112.024059. Epub 2012 Dec 11. 10.1074/mcp.O112.024059 PubMed 23233447