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EGFPC1-hMyoX
(Plasmid #47608)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47608 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    EGFPC1
  • Backbone manufacturer
    Clontech; modified as described in Rogers et al JBC 2001
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MyoX
  • Alt name
    MYO10
  • Alt name
    myosin X
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6167
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP-HA-His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFPC1-hMyoX was a gift from Emanuel Strehler (Addgene plasmid # 47608)
  • For your References section:

    Calmodulin-like protein increases filopodia-dependent cell motility via up-regulation of myosin-10. Bennett RD, Mauer AS, Strehler EE. J Biol Chem. 2007 Feb 2;282(5):3205-12. Epub 2006 Nov 26. 10.1074/jbc.M607174200 PubMed 17130134