Danio rerio cryabb
(Plasmid #47665)

• Purpose: Recombinant protein production
• Depositing Lab: Mason Posner
• Publication: Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 2012 Mar 29.

Backbone

• Vector backbone: pET-21b(+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BL21(DE3)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Alpha B-crystallin
• Alt name: cryabb
• Species: D. rerio (zebrafish)
• Insert Size (bp): 558
• GenBank ID: BC076518.1 DQ113417.1
• Entrez Gene: cryabb (a.k.a. cryab2, wu:fe37f08, zgc:91937)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's full plasmid sequencing results found that the plasmid backbone differs from what is reported in the associated publication, however, the change in backbone is not expected to affect the use of the plasmids to produce recombinant protein.