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pGEXndoE
(Plasmid #47714)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47714 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-5X-3
  • Backbone manufacturer
    GE Helthcare
  • Backbone size w/o insert (bp) 4974
  • Total vector size (bp) 7306
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    use BL21 derivative for protein expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ndoE
  • Alt name
    ndoE
  • Species
    Entercoccus faecalis
  • Insert Size (bp)
    2350
  • GenBank ID
    AY376354
  • Promoter tac
  • Tag / Fusion Protein
    • Glutathione S-transferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GGGCTGGCAAGCCACGTTTGGTG
  • 3′ sequencing primer CCGGGAGCTGCATGTGTCAGAGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEXndoE was a gift from Mattias Collin & Vincent Fischetti (Addgene plasmid # 47714 ; http://n2t.net/addgene:47714 ; RRID:Addgene_47714)
  • For your References section:

    A novel secreted endoglycosidase from Enterococcus faecalis with activity on human immunoglobulin G and ribonuclease B. Collin M, Fischetti VA. J Biol Chem. 2004 May 21;279(21):22558-70. Epub 2004 Mar 17. 10.1074/jbc.M402156200 PubMed 15028731