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mCherry-hPMCA2xb
(Plasmid #47751)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47751 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    EGFP replaced with mCherry as Age/KpnI fragment
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PMCA2x/b
  • Alt name
    ATPase, Ca++ transporting, plasma membrane 2
  • Alt name
    ATP2B2
  • Species
    H. sapiens (human)
  • Mutation
    xb splice variant
  • Entrez Gene
    ATP2B2 (a.k.a. DFNA82, PMCA2, PMCA2a, PMCA2i)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer mCherry F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Similar to Addgene plasmid 47585 except EGFP replaced with mCherry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-hPMCA2xb was a gift from Emanuel Strehler (Addgene plasmid # 47751 ; http://n2t.net/addgene:47751 ; RRID:Addgene_47751)
  • For your References section:

    Plasma membrane Ca(2)(+)-ATPases can shape the pattern of Ca(2)(+) transients induced by store-operated Ca(2)(+) entry. Paszty K, Caride AJ, Bajzer Z, Offord CP, Padanyi R, Hegedus L, Varga K, Strehler EE, Enyedi A. Sci Signal. 2015 Feb 17;8(364):ra19. doi: 10.1126/scisignal.2005672. 10.1126/scisignal.2005672 PubMed 25690014