pMM2-hPMCA4a
(Plasmid #47761)

• Purpose: mammalian expression of human PMCA4a
• Depositing Labs: John Penniston, Emanuel Strehler
• Publication: Enyedi et al J Biol Chem. 1994 Jan 7;269(1):41-3.

Backbone

• Vector backbone: pMM2
• Backbone manufacturer: Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PMCA4a
• Alt name: ATP2B4
• Alt name: ATPase, Ca++ transporting, plasma membrane 4
• Species: H. sapiens (human)
• Entrez Gene: ATP2B4 (a.k.a. ATP2B2, MXRA1, PMCA4, PMCA4b, PMCA4x)
• Promoter: SV40

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: pMT2-F
• 3′ sequencing primer: pMT2-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that PMCA4 residue 2 is Ala (instead of Thr) due to an optimized initiation sequence (ATG GCG). The alternatively spliced 178 nt exon sequence is at positions 4377-4554.