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pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)
(Plasmid #47870)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47870 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSimpleII
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 8501
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    NmCas9
  • Alt name
    CSN1
  • Species
    N meningitidis
  • Insert Size (bp)
    3243
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    • HA (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    U6pr-tracrRNA
  • Species
    N meningitidis
  • Insert Size (bp)
    364

Gene/Insert 3

  • Gene/Insert name
    OCT4 crRNA
  • Species
    Synthetic

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s) was a gift from Erik Sontheimer & James Thomson (Addgene plasmid # 47870 ; http://n2t.net/addgene:47870 ; RRID:Addgene_47870)
  • For your References section:

    Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA. Proc Natl Acad Sci U S A. 2013 Aug 12. 10.1073/pnas.1313587110 PubMed 23940360