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pIK86
(Plasmid #47933)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47933 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2730
  • Total vector size (bp) 7347
  • Vector type
    CRISPR, Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4617
  • Promoter T7

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The Cas9 insert was commercially synthesized by Genscript, on our request.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIK86 was a gift from Iskra Katic (Addgene plasmid # 47933 ; http://n2t.net/addgene:47933 ; RRID:Addgene_47933)
  • For your References section:

    Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans. Katic I, Grosshans H. Genetics. 2013 Aug 26. 10.1534/genetics.113.155754 PubMed 23979578