PurposeC. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47933||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2730
- Total vector size (bp) 7347
Vector typeCRISPR, Unspecified
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)4617
- Promoter T7
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIK86 was a gift from Iskra Katic (Addgene plasmid # 47933 ; http://n2t.net/addgene:47933 ; RRID:Addgene_47933)
For your References section:Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans. Katic I, Grosshans H. Genetics. 2013 Aug 26. 10.1534/genetics.113.155754 PubMed 23979578