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pSpCas9(BB)-2A-GFP (PX458) Citations (577)

Plasmid Article: Genome engineering using the CRISPR-Cas9 system.
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
PubMed Journal

Articles Citing pSpCas9(BB)-2A-GFP (PX458)

Articles
Antiviral immunity via RIG-I-mediated recognition of RNA bearing 5'-diphosphates. Goubau D, Schlee M, Deddouche S, Pruijssers AJ, Zillinger T, Goldeck M, Schuberth C, Van der Veen AG, Fujimura T, Rehwinkel J, Iskarpatyoti JA, Barchet W, Ludwig J, Dermody TS, Hartmann G, Reis e Sousa C. Nature. 2014 Oct 16;514(7522):372-5. doi: 10.1038/nature13590. Epub 2014 Aug 10. PubMed
Saturation editing of genomic regions by multiplex homology-directed repair. Findlay GM, Boyle EA, Hause RJ, Klein JC, Shendure J. Nature. 2014 Sep 4;513(7516):120-3. doi: 10.1038/nature13695. PubMed
TALEN/CRISPR-mediated eGFP knock-in add-on at the OCT4 locus does not impact differentiation of human embryonic stem cells towards endoderm. Krentz NA, Nian C, Lynn FC. PLoS One. 2014 Dec 4;9(12):e114275. doi: 10.1371/journal.pone.0114275. eCollection 2014. PubMed
Generation of genomic deletions in mammalian cell lines via CRISPR/Cas9. Bauer DE, Canver MC, Orkin SH. J Vis Exp. 2014 Jan 3;(95). doi: 10.3791/52118. PubMed
FoxO3a suppresses the senescence of cardiac microvascular endothelial cells by regulating the ROS-mediated cell cycle. Qi XF, Chen ZY, Xia JB, Zheng L, Zhao H, Pi LQ, Park KS, Kim SK, Lee KJ, Cai DQ. J Mol Cell Cardiol. 2015 Apr;81:114-26. doi: 10.1016/j.yjmcc.2015.01.022. Epub 2015 Feb 2. PubMed
Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool. Wettstein R, Bodak M, Ciaudo C. Methods Mol Biol. 2015 Mar 12. PubMed
Hepatitis C virus RNA functionally sequesters miR-122. Luna JM, Scheel TK, Danino T, Shaw KS, Mele A, Fak JJ, Nishiuchi E, Takacs CN, Catanese MT, de Jong YP, Jacobson IM, Rice CM, Darnell RB. Cell. 2015 Mar 12;160(6):1099-110. doi: 10.1016/j.cell.2015.02.025. PubMed
Selective Distal Enhancer Control of the Mmp13 Gene Identified through Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Genomic Deletions. Meyer MB, Benkusky NA, Pike JW. J Biol Chem. 2015 Apr 24;290(17):11093-107. doi: 10.1074/jbc.M115.648394. Epub 2015 Mar 15. PubMed
Context-specific function of the LIM homeobox 1 transcription factor in head formation of the mouse embryo. Fossat N, Ip CK, Jones VJ, Studdert JB, Khoo PL, Lewis SL, Power M, Tourle K, Loebel DA, Kwan KM, Behringer RR, Tam PP. Development. 2015 Jun 1;142(11):2069-79. doi: 10.1242/dev.120907. Epub 2015 May 14. PubMed
Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems. Bialk P, Rivera-Torres N, Strouse B, Kmiec EB. PLoS One. 2015 Jun 8;10(6):e0129308. doi: 10.1371/journal.pone.0129308. eCollection 2015. PubMed
Protein Kinase A Opposes the Phosphorylation-Dependent Recruitment of Glycogen Synthase Kinase 3beta to A-Kinase Anchoring Protein 220. Whiting JL, Nygren PJ, Tunquist BJ, Langeberg LK, Seternes OM, Scott JD. J Biol Chem. 2015 Jun 18. pii: jbc.M115.654822. PubMed
Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes. Hobbs RP, DePianto DJ, Jacob JT, Han MC, Chung BM, Batazzi AS, Poll BG, Guo Y, Han J, Ong S, Zheng W, Taube JM, Cihakova D, Wan F, Coulombe PA. Nat Genet. 2015 Aug;47(8):933-8. doi: 10.1038/ng.3355. Epub 2015 Jul 13. PubMed
Generation of a conditional analog-sensitive kinase in human cells using CRISPR/Cas9-mediated genome engineering. Moyer TC, Holland AJ. Methods Cell Biol. 2015;129:19-36. doi: 10.1016/bs.mcb.2015.03.017. Epub 2015 May 27. PubMed
CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM. Genome Res. 2015 Sep 9. PubMed

Associated Plasmids

Impaired PIEZO1 function in patients with a novel autosomal recessive congenital lymphatic dysplasia. Lukacs V, Mathur J, Mao R, Bayrak-Toydemir P, Procter M, Cahalan SM, Kim HJ, Bandell M, Longo N, Day RW, Stevenson DA, Patapoutian A, Krock BL. Nat Commun. 2015 Sep 21;6:8329. doi: 10.1038/ncomms9329. PubMed
Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin. Vinckevicius A, Parker JB, Chakravarti D. Mol Cell Biol. 2015 Dec 15;35(24):4135-46. doi: 10.1128/MCB.00477-15. Epub 2015 Sep 28. PubMed
The adherens junctions control susceptibility to Staphylococcus aureus alpha-toxin. Popov LM, Marceau CD, Starkl PM, Lumb JH, Shah J, Guerrera D, Cooper RL, Merakou C, Bouley DM, Meng W, Kiyonari H, Takeichi M, Galli SJ, Bagnoli F, Citi S, Carette JE, Amieva MR. Proc Natl Acad Sci U S A. 2015 Nov 17;112(46):14337-42. doi: 10.1073/pnas.1510265112. Epub 2015 Oct 21. PubMed
Mechanisms of Enhancer-mediated Hormonal Control of Vitamin D Receptor Gene Expression in Target Cells. Lee SM, Meyer MB, Benkusky NA, O'Brien CA, Pike JW. J Biol Chem. 2015 Dec 18;290(51):30573-86. doi: 10.1074/jbc.M115.693614. Epub 2015 Oct 25. PubMed
Integrin alpha4beta1 controls G9a activity that regulates epigenetic changes and nuclear properties required for lymphocyte migration. Zhang X, Cook PC, Zindy E, Williams CJ, Jowitt TA, Streuli CH, MacDonald AS, Redondo-Munoz J. Nucleic Acids Res. 2015 Dec 10. pii: gkv1348. PubMed
A facile one-step strategy for the generation of conditional knockout mice to explore the role of Notch1 in oroesophageal tumorigenesis. Mandasari M, Sawangarun W, Katsube K, Kayamori K, Yamaguchi A, Sakamoto K. Biochem Biophys Res Commun. 2016 Jan 15;469(3):761-7. doi: 10.1016/j.bbrc.2015.12.006. Epub 2015 Dec 9. PubMed
Spell Checking Nature: Versatility of CRISPR/Cas9 for Developing Treatments for Inherited Disorders. Wojtal D, Kemaladewi DU, Malam Z, Abdullah S, Wong TW, Hyatt E, Baghestani Z, Pereira S, Stavropoulos J, Mouly V, Mamchaoui K, Muntoni F, Voit T, Gonorazky HD, Dowling JJ, Wilson MD, Mendoza-Londono R, Ivakine EA, Cohn RD. Am J Hum Genet. 2016 Jan 7;98(1):90-101. doi: 10.1016/j.ajhg.2015.11.012. Epub 2015 Dec 10. PubMed
Yap and Taz play a crucial role in neural crest-derived craniofacial development. Wang J, Xiao Y, Hsu CW, Martinez-Traverso IM, Zhang M, Bai Y, Ishii M, Maxson RE, Olson EN, Dickinson ME, Wythe JD, Martin JF. Development. 2016 Feb 1;143(3):504-15. doi: 10.1242/dev.126920. Epub 2015 Dec 30. PubMed
A mouse model for adult cardiac-specific gene deletion with CRISPR/Cas9. Carroll KJ, Makarewich CA, McAnally J, Anderson DM, Zentilin L, Liu N, Giacca M, Bassel-Duby R, Olson EN. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):338-43. doi: 10.1073/pnas.1523918113. Epub 2015 Dec 30. PubMed
Distinct effects of beta1 integrin on cell proliferation and cellular signaling in MDA-MB-231 breast cancer cells. Hou S, Isaji T, Hang Q, Im S, Fukuda T, Gu J. Sci Rep. 2016 Jan 5;6:18430. doi: 10.1038/srep18430. PubMed
Comparison of surrogate reporter systems for enrichment of cells with mutations induced by genome editors. He Z, Shi X, Liu M, Sun G, Proudfoot C, Whitelaw CB, Lillico SG, Chen Y. J Biotechnol. 2016 Jan 14;221:49-54. doi: 10.1016/j.jbiotec.2016.01.009. PubMed
Efficient Restoration of the Dystrophin Gene Reading Frame and Protein Structure in DMD Myoblasts Using the CinDel Method. Iyombe-Engembe JP, Ouellet DL, Barbeau X, Rousseau J, Chapdelaine P, Lague P, Tremblay JP. Mol Ther Nucleic Acids. 2016 Jan 26;5:e283. doi: 10.1038/mtna.2015.58. PubMed
An essential receptor for adeno-associated virus infection. Pillay S, Meyer NL, Puschnik AS, Davulcu O, Diep J, Ishikawa Y, Jae LT, Wosen JE, Nagamine CM, Chapman MS, Carette JE. Nature. 2016 Feb 4;530(7588):108-12. doi: 10.1038/nature16465. Epub 2016 Jan 27. PubMed
Mitotic Diversity in Homeostatic Human Interfollicular Epidermis. Noske K, Stark HJ, Nevaril L, Berning M, Langbein L, Goyal A, Diederichs S, Boukamp P. Int J Mol Sci. 2016 Jan 28;17(2). pii: E167. doi: 10.3390/ijms17020167. PubMed
Phosphorylation-Dependent Regulation of the DNA Damage Response of Adaptor Protein KIBRA in Cancer Cells. Mavuluri J, Beesetti S, Surabhi R, Kremerskothen J, Venkatraman G, Rayala SK. Mol Cell Biol. 2016 Apr 15;36(9):1354-65. doi: 10.1128/MCB.01004-15. Print 2016 May 1. PubMed
AMPK governs lineage specification through Tfeb-dependent regulation of lysosomes. Young NP, Kamireddy A, Van Nostrand JL, Eichner LJ, Shokhirev MN, Dayn Y, Shaw RJ. Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115. PubMed

Associated Plasmids

Psychiatric Risk Gene Transcription Factor 4 Regulates Intrinsic Excitability of Prefrontal Neurons via Repression of SCN10a and KCNQ1. Rannals MD, Hamersky GR, Page SC, Campbell MN, Briley A, Gallo RA, Phan BN, Hyde TM, Kleinman JE, Shin JH, Jaffe AE, Weinberger DR, Maher BJ. Neuron. 2016 Apr 6;90(1):43-55. doi: 10.1016/j.neuron.2016.02.021. Epub 2016 Mar 10. PubMed
Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, Yeo GW. Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 10.1016/j.cell.2016.02.054. PubMed

Associated Plasmids

PLEKHA7 Recruits PDZD11 to Adherens Junctions to Stabilize Nectins. Guerrera D, Shah J, Vasileva E, Sluysmans S, Mean I, Jond L, Poser I, Mann M, Hyman AA, Citi S. J Biol Chem. 2016 May 20;291(21):11016-29. doi: 10.1074/jbc.M115.712935. Epub 2016 Apr 4. PubMed
Inhibition of HSV-1 Replication by Gene Editing Strategy. Roehm PC, Shekarabi M, Wollebo HS, Bellizzi A, He L, Salkind J, Khalili K. Sci Rep. 2016 Apr 11;6:23146. doi: 10.1038/srep23146. PubMed
Histone H3 globular domain acetylation identifies a new class of enhancers. Pradeepa MM, Grimes GR, Kumar Y, Olley G, Taylor GC, Schneider R, Bickmore WA. Nat Genet. 2016 Jun;48(6):681-6. doi: 10.1038/ng.3550. Epub 2016 Apr 18. PubMed
CRISPR/Cas9 knockout of HAS2 in rat chondrosarcoma chondrocytes demonstrates the requirement of hyaluronan for aggrecan retention. Huang Y, Askew EB, Knudson CB, Knudson W. Matrix Biol. 2016 Dec;56:74-94. doi: 10.1016/j.matbio.2016.04.002. Epub 2016 Apr 14. PubMed
Sphingosine-1-Phosphate Lyase Deficient Cells as a Tool to Study Protein Lipid Interactions. Gerl MJ, Bittl V, Kirchner S, Sachsenheimer T, Brunner HL, Luchtenborg C, Ozbalci C, Wiedemann H, Wegehingel S, Nickel W, Haberkant P, Schultz C, Kruger M, Brugger B. PLoS One. 2016 Apr 21;11(4):e0153009. doi: 10.1371/journal.pone.0153009. eCollection 2016. PubMed
ISL1 and JMJD3 synergistically control cardiac differentiation of embryonic stem cells. Wang Y, Li Y, Guo C, Lu Q, Wang W, Jia Z, Chen P, Ma K, Reinberg D, Zhou C. Nucleic Acids Res. 2016 Apr 21. pii: gkw301. PubMed
Expression of MLL-AF4 or AF4-MLL fusions does not impact the efficiency of DNA damage repair. Castano J, Herrero AB, Bursen A, Gonzalez F, Marschalek R, Gutierrez NC, Menendez P. Oncotarget. 2016 May 24;7(21):30440-52. doi: 10.18632/oncotarget.8938. PubMed
Plet1 is an epigenetically regulated cell surface protein that provides essential cues to direct trophoblast stem cell differentiation. Murray A, Sienerth AR, Hemberger M. Sci Rep. 2016 Apr 28;6:25112. doi: 10.1038/srep25112. PubMed
AMPK Causes Cell Cycle Arrest in LKB1-deficient Cells via Activation of CAMKK2. Fogarty S, Ross FA, Vara Ciruelos D, Gray A, Gowans GJ, Hardie DG. Mol Cancer Res. 2016 May 2. pii: molcanres.0479.2015. PubMed
The recombined cccDNA produced using minicircle technology mimicked HBV genome in structure and function closely. Guo X, Chen P, Hou X, Xu W, Wang D, Wang TY, Zhang L, Zheng G, Gao ZL, He CY, Zhou B, Chen ZY. Sci Rep. 2016 May 13;6:25552. doi: 10.1038/srep25552. PubMed
The DNA Structure-Specific Endonuclease MUS81 Mediates DNA Sensor STING-Dependent Host Rejection of Prostate Cancer Cells. Ho SS, Zhang WY, Tan NY, Khatoo M, Suter MA, Tripathi S, Cheung FS, Lim WK, Tan PH, Ngeow J, Gasser S. Immunity. 2016 May 17;44(5):1177-89. doi: 10.1016/j.immuni.2016.04.010. Epub 2016 May 10. PubMed
The Evx1/Evx1as gene locus regulates anterior-posterior patterning during gastrulation. Bell CC, Amaral PP, Kalsbeek A, Magor GW, Gillinder KR, Tangermann P, di Lisio L, Cheetham SW, Gruhl F, Frith J, Tallack MR, Ru KL, Crawford J, Mattick JS, Dinger ME, Perkins AC. Sci Rep. 2016 May 26;6:26657. doi: 10.1038/srep26657. PubMed
Pitx2 promotes heart repair by activating the antioxidant response after cardiac injury. Tao G, Kahr PC, Morikawa Y, Zhang M, Rahmani M, Heallen TR, Li L, Sun Z, Olson EN, Amendt BA, Martin JF. Nature. 2016 Jun 2;534(7605):119-23. doi: 10.1038/nature17959. PubMed
Aberrant PD-L1 expression through 3'-UTR disruption in multiple cancers. Kataoka K, Shiraishi Y, Takeda Y, Sakata S, Matsumoto M, Nagano S, Maeda T, Nagata Y, Kitanaka A, Mizuno S, Tanaka H, Chiba K, Ito S, Watatani Y, Kakiuchi N, Suzuki H, Yoshizato T, Yoshida K, Sanada M, Itonaga H, Imaizumi Y, Totoki Y, Munakata W, Nakamura H, Hama N, Shide K, Kubuki Y, Hidaka T, Kameda T, Masuda K, Minato N, Kashiwase K, Izutsu K, Takaori-Kondo A, Miyazaki Y, Takahashi S, Shibata T, Kawamoto H, Akatsuka Y, Shimoda K, Takeuchi K, Seya T, Miyano S, Ogawa S. Nature. 2016 Jun 16;534(7607):402-6. doi: 10.1038/nature18294. PubMed
Combination of the clustered regularly interspaced short palindromic repeats (CRISPR)-associated 9 technique with the piggybac transposon system for mouse in utero electroporation to study cortical development. Cheng M, Jin X, Mu L, Wang F, Li W, Zhong X, Liu X, Shen W, Liu Y, Zhou Y. J Neurosci Res. 2016 Jun 18. doi: 10.1002/jnr.23776. PubMed
TCF7L1 Modulates Colorectal Cancer Growth by Inhibiting Expression of the Tumor-Suppressor Gene EPHB3. Murphy M, Chatterjee SS, Jain S, Katari M, DasGupta R. Sci Rep. 2016 Jun 23;6:28299. doi: 10.1038/srep28299. PubMed
Opposing Roles of Double-Stranded RNA Effector Pathways and Viral Defense Proteins Revealed with CRISPR/Cas9 Knock-Out Cell Lines and Vaccinia Virus Mutants. Liu R, Moss B. J Virol. 2016 Jun 22. pii: JVI.00869-16. PubMed
AP1S3 Mutations Cause Skin Autoinflammation by Disrupting Keratinocyte Autophagy and Up-Regulating IL-36 Production. Mahil SK, Twelves S, Farkas K, Setta-Kaffetzi N, Burden AD, Gach JE, Irvine AD, Kepiro L, Mockenhaupt M, Oon HH, Pinner J, Ranki A, Seyger MM, Soler-Palacin P, Storan ER, Tan ES, Valeyrie-Allanore L, Young HS, Trembath RC, Choon SE, Szell M, Bata-Csorgo Z, Smith CH, Di Meglio P, Barker JN, Capon F. J Invest Dermatol. 2016 Nov;136(11):2251-2259. doi: 10.1016/j.jid.2016.06.618. Epub 2016 Jul 5. PubMed
Identification and validation of regulatory SNPs that modulate transcription factor chromatin binding and gene expression in prostate cancer. Jin HJ, Jung S, DebRoy AR, Davuluri RV. Oncotarget. 2016 Aug 23;7(34):54616-54626. doi: 10.18632/oncotarget.10520. PubMed
A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. Lambrus BG, Daggubati V, Uetake Y, Scott PM, Clutario KM, Sluder G, Holland AJ. J Cell Biol. 2016 Jul 18;214(2):143-53. doi: 10.1083/jcb.201604054. PubMed
Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells. Shy BR, MacDougall MS, Clarke R, Merrill BJ. Nucleic Acids Res. 2016 Aug 2. pii: gkw685. PubMed
Biallelic inactivation of REV7 is associated with Fanconi anemia. Bluteau D, Masliah-Planchon J, Clairmont C, Rousseau A, Ceccaldi R, Dubois d'Enghien C, Bluteau O, Cuccuini W, Gachet S, Peffault de Latour R, Leblanc T, Socie G, Baruchel A, Stoppa-Lyonnet D, D'Andrea AD, Soulier J. J Clin Invest. 2016 Sep 1;126(9):3580-4. doi: 10.1172/JCI88010. Epub 2016 Aug 8. PubMed
Chromatin-Remodeling Complex SWI/SNF Controls Multidrug Resistance by Transcriptionally Regulating the Drug Efflux Pump ABCB1. Dubey R, Lebensohn AM, Bahrami-Nejad Z, Marceau C, Champion M, Gevaert O, Sikic BI, Carette JE, Rohatgi R. Cancer Res. 2016 Oct 1;76(19):5810-5821. Epub 2016 Aug 8. PubMed
Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth. Khanom R, Nguyen CT, Kayamori K, Zhao X, Morita K, Miki Y, Katsube K, Yamaguchi A, Sakamoto K. PLoS One. 2016 Aug 11;11(8):e0161163. doi: 10.1371/journal.pone.0161163. eCollection 2016. PubMed
A genome-editing strategy to treat beta-hemoglobinopathies that recapitulates a mutation associated with a benign genetic condition. Traxler EA, Yao Y, Wang YD, Woodard KJ, Kurita R, Nakamura Y, Hughes JR, Hardison RC, Blobel GA, Li C, Weiss MJ. Nat Med. 2016 Sep;22(9):987-90. doi: 10.1038/nm.4170. Epub 2016 Aug 15. PubMed
The long non-coding RNA Morrbid regulates Bim and short-lived myeloid cell lifespan. Kotzin JJ, Spencer SP, McCright SJ, Kumar DB, Collet MA, Mowel WK, Elliott EN, Uyar A, Makiya MA, Dunagin MC, Harman CC, Virtue AT, Zhu S, Bailis W, Stein J, Hughes C, Raj A, Wherry EJ, Goff LA, Klion AD, Rinn JL, Williams A, Flavell RA, Henao-Mejia J. Nature. 2016 Sep 8;537(7619):239-243. doi: 10.1038/nature19346. Epub 2016 Aug 15. PubMed
Telomeric RNAs are essential to maintain telomeres. Montero JJ, Lopez de Silanes I, Grana O, Blasco MA. Nat Commun. 2016 Aug 17;7:12534. doi: 10.1038/ncomms12534. PubMed
Immunogenomic engineering of a plug-and-(dis)play hybridoma platform. Pogson M, Parola C, Kelton WJ, Heuberger P, Reddy ST. Nat Commun. 2016 Aug 17;7:12535. doi: 10.1038/ncomms12535. PubMed
Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Santos DP, Kiskinis E, Eggan K, Merkle FT. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15. PubMed
PLEKHG3 enhances polarized cell migration by activating actin filaments at the cell front. Nguyen TT, Park WS, Park BO, Kim CY, Oh Y, Kim JM, Choi H, Kyung T, Kim CH, Lee G, Hahn KM, Meyer T, Heo WD. Proc Natl Acad Sci U S A. 2016 Sep 6;113(36):10091-6. doi: 10.1073/pnas.1604720113. Epub 2016 Aug 23. PubMed
An endosomal tether undergoes an entropic collapse to bring vesicles together. Murray DH, Jahnel M, Lauer J, Avellaneda MJ, Brouilly N, Cezanne A, Morales-Navarrete H, Perini ED, Ferguson C, Lupas AN, Kalaidzidis Y, Parton RG, Grill SW, Zerial M. Nature. 2016 Sep 1;537(7618):107-111. doi: 10.1038/nature19326. Epub 2016 Aug 24. PubMed
Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides. Bialk P, Sansbury B, Rivera-Torres N, Bloh K, Man D, Kmiec EB. Sci Rep. 2016 Sep 9;6:32681. doi: 10.1038/srep32681. PubMed
Accessory subunits are integral for assembly and function of human mitochondrial complex I. Stroud DA, Surgenor EE, Formosa LE, Reljic B, Frazier AE, Dibley MG, Osellame LD, Stait T, Beilharz TH, Thorburn DR, Salim A, Ryan MT. Nature. 2016 Oct 6;538(7623):123-126. doi: 10.1038/nature19754. Epub 2016 Sep 14. PubMed
Tumor-specific CD4+ T cells eradicate myeloma cells genetically deficient in MHC class II display. Tveita A, Fauskanger M, Bogen B, Haabeth OA. Oncotarget. 2016 Oct 11;7(41):67175-67182. doi: 10.18632/oncotarget.11946. PubMed
Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel H, Radbruch A, Krause E, Baumgrass R. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326. PubMed

Associated Plasmids

Direct reprogramming of urine-derived cells with inducible MyoD for modeling human muscle disease. Kim EY, Page P, Dellefave-Castillo LM, McNally EM, Wyatt EJ. Skelet Muscle. 2016 Sep 15;6:32. doi: 10.1186/s13395-016-0103-9. eCollection 2016. PubMed
A Recurrent ERCC3 Truncating Mutation Confers Moderate Risk for Breast Cancer. Vijai J, Topka S, Villano D, Ravichandran V, Maxwell KN, Maria A, Thomas T, Gaddam P, Lincoln A, Kazzaz S, Wenz B, Carmi S, Schrader KA, Hart SN, Lipkin SM, Neuhausen SL, Walsh MF, Zhang L, Lejbkowicz F, Rennert H, Stadler ZK, Robson M, Weitzel JN, Domchek S, Daly MJ, Couch FJ, Nathanson KL, Norton L, Rennert G, Offit K. Cancer Discov. 2016 Nov;6(11):1267-1275. Epub 2016 Sep 21. PubMed
TRIMs and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-direct Autophagy in Endomembrane Damage Homeostasis. Chauhan S, Kumar S, Jain A, Ponpuak M, Mudd MH, Kimura T, Choi SW, Peters R, Mandell M, Bruun JA, Johansen T, Deretic V. Dev Cell. 2016 Oct 10;39(1):13-27. doi: 10.1016/j.devcel.2016.08.003. Epub 2016 Sep 29. PubMed
Activation of Notch1 synergizes with multiple pathways in promoting castration-resistant prostate cancer. Stoyanova T, Riedinger M, Lin S, Faltermeier CM, Smith BA, Zhang KX, Going CC, Goldstein AS, Lee JK, Drake JM, Rice MA, Hsu EC, Nowroozizadeh B, Castor B, Orellana SY, Blum SM, Cheng D, Pienta KJ, Reiter RE, Pitteri SJ, Huang J, Witte ON. Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):E6457-E6466. Epub 2016 Sep 30. PubMed
Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Goyal A, Myacheva K, Gross M, Klingenberg M, Duran Arque B, Diederichs S. Nucleic Acids Res. 2016 Sep 30. pii: gkw883. PubMed
PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF. Tadi SK, Tellier-Lebegue C, Nemoz C, Drevet P, Audebert S, Roy S, Meek K, Charbonnier JB, Modesti M. Cell Rep. 2016 Oct 4;17(2):541-555. doi: 10.1016/j.celrep.2016.09.026. PubMed
Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naive pluripotency by complementary mechanisms. Hore TA, von Meyenn F, Ravichandran M, Bachman M, Ficz G, Oxley D, Santos F, Balasubramanian S, Jurkowski TP, Reik W. Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12202-12207. Epub 2016 Oct 11. PubMed
The poly(ADP-ribose)-dependent chromatin remodeler Alc1 induces local chromatin relaxation upon DNA damage. Sellou H, Lebeaupin T, Chapuis C, Smith R, Hegele A, Singh HR, Kozlowski M, Bultmann S, Ladurner AG, Timinszky G, Huet S. Mol Biol Cell. 2016 Dec 1;27(24):3791-3799. Epub 2016 Oct 12. PubMed
Essential role of the Dishevelled DEP domain in a Wnt-dependent human-cell-based complementation assay. Gammons MV, Rutherford TJ, Steinhart Z, Angers S, Bienz M. J Cell Sci. 2016 Oct 15;129(20):3892-3902. PubMed
SIKs control osteocyte responses to parathyroid hormone. Wein MN, Liang Y, Goransson O, Sundberg TB, Wang J, Williams EA, O'Meara MJ, Govea N, Beqo B, Nishimori S, Nagano K, Brooks DJ, Martins JS, Corbin B, Anselmo A, Sadreyev R, Wu JY, Sakamoto K, Foretz M, Xavier RJ, Baron R, Bouxsein ML, Gardella TJ, Divieti-Pajevic P, Gray NS, Kronenberg HM. Nat Commun. 2016 Oct 19;7:13176. doi: 10.1038/ncomms13176. PubMed
Non-SMC Element 2 (NSMCE2) of the SMC5/6 Complex Helps to Resolve Topological Stress. Verver DE, Zheng Y, Speijer D, Hoebe R, Dekker HL, Repping S, Stap J, Hamer G. Int J Mol Sci. 2016 Oct 26;17(11). pii: ijms17111782. doi: 10.3390/ijms17111782. PubMed
S2 from equine infectious anemia virus is an infectivity factor which counteracts the retroviral inhibitors SERINC5 and SERINC3. Chande A, Cuccurullo EC, Rosa A, Ziglio S, Carpenter S, Pizzato M. Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):13197-13202. Epub 2016 Nov 1. PubMed
Paradoxical Sensitivity to an Integrated Stress Response Blocking Mutation in Vanishing White Matter Cells. Sekine Y, Zyryanova A, Crespillo-Casado A, Amin-Wetzel N, Harding HP, Ron D. PLoS One. 2016 Nov 3;11(11):e0166278. doi: 10.1371/journal.pone.0166278. eCollection 2016. PubMed
CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening. Koferle A, Worf K, Breunig C, Baumann V, Herrero J, Wiesbeck M, Hutter LH, Gotz M, Fuchs C, Beck S, Stricker SH. BMC Genomics. 2016 Nov 14;17(1):917. PubMed
A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a coactivator of NQO1 in sulforaphane-treated colon cancer cells. Johnson GS, Li J, Beaver LM, Dashwood WM, Sun D, Rajendran P, Williams DE, Ho E, Dashwood RH. Mol Nutr Food Res. 2016 Nov 18. doi: 10.1002/mnfr.201600769. PubMed
Atg8 family LC3/GABARAP proteins are crucial for autophagosome-lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation. Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M. J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18. PubMed

Associated Plasmids

A Functional Variant Associated with Atrial Fibrillation Regulates PITX2c Expression through TFAP2a. Ye J, Tucker NR, Weng LC, Clauss S, Lubitz SA, Ellinor PT. Am J Hum Genet. 2016 Dec 1;99(6):1281-1291. doi: 10.1016/j.ajhg.2016.10.001. Epub 2016 Nov 17. PubMed
Par3L enhances colorectal cancer cell survival by inhibiting Lkb1/AMPK signaling pathway. Li T, Liu D, Lei X, Jiang Q. Biochem Biophys Res Commun. 2017 Jan 22;482(4):1037-1041. doi: 10.1016/j.bbrc.2016.11.154. Epub 2016 Nov 28. PubMed
Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro. Termglinchan V, Seeger T, Chen C, Wu JC, Karakikes I. Methods Mol Biol. 2017;1521:55-68. PubMed
Roles for APRIN (PDS5B) in homologous recombination and in ovarian cancer prediction. Couturier AM, Fleury H, Patenaude AM, Bentley VL, Rodrigue A, Coulombe Y, Niraj J, Pauty J, Berman JN, Dellaire G, Di Noia JM, Mes-Masson AM, Masson JY. Nucleic Acids Res. 2016 Dec 15;44(22):10879-10897. Epub 2016 Oct 24. PubMed
Integrated Genome and Protein Editing Swaps alpha-2,6 Sialylation for alpha-2,3 Sialic Acid on Recombinant Antibodies from CHO. Chung CY, Wang Q, Yang S, Yin B, Zhang H, Betenbaugh M. Biotechnol J. 2016 Dec 12. doi: 10.1002/biot.201600502. PubMed
Efficient and versatile CRISPR engineering of human neurons in culture to model neurological disorders. Shah RR, Cholewa-Waclaw J, Davies FC, Paton KM, Chaligne R, Heard E, Abbott CM, Bird AP. Wellcome Open Res. 2016 Nov 15;1:13. PubMed
A novel isoform of the human mitochondrial complex I subunit NDUFV3. Dibley MG, Ryan MT, Stroud DA. FEBS Lett. 2017 Jan;591(1):109-117. doi: 10.1002/1873-3468.12527. Epub 2016 Dec 30. PubMed
Macrophages Enhance Migration in Inflammatory Breast Cancer Cells via RhoC GTPase Signaling. Allen SG, Chen YC, Madden JM, Fournier CL, Altemus MA, Hiziroglu AB, Cheng YH, Wu ZF, Bao L, Yates JA, Yoon E, Merajver SD. Sci Rep. 2016 Dec 19;6:39190. doi: 10.1038/srep39190. PubMed
IRBIT controls apoptosis by interacting with the Bcl-2 homolog, Bcl2l10, and by promoting ER-mitochondria contact. Bonneau B, Ando H, Kawaai K, Hirose M, Takahashi-Iwanaga H, Mikoshiba K. Elife. 2016 Dec 20;5. pii: e19896. doi: 10.7554/eLife.19896. PubMed
CRISPR/Cas9-mediated integration enables TAG-eCLIP of endogenously tagged RNA binding proteins. Van Nostrand EL, Gelboin-Burkhart C, Wang R, Pratt GA, Blue SM, Yeo GW. Methods. 2016 Dec 18. pii: S1046-2023(16)30481-9. doi: 10.1016/j.ymeth.2016.12.007. PubMed
BNC2 is a putative tumor suppressor gene in high-grade serous ovarian carcinoma and impacts cell survival after oxidative stress. Cesaratto L, Grisard E, Coan M, Zandona L, De Mattia E, Poletto E, Cecchin E, Puglisi F, Canzonieri V, Mucignat MT, Zucchetto A, Stocco G, Colombatti A, Nicoloso MS, Spizzo R. Cell Death Dis. 2016 Dec 22;7(12):e2526. doi: 10.1038/cddis.2016.448. PubMed
Effects of a human recombinant alkaline phosphatase during impaired mitochondrial function in human renal proximal tubule epithelial cells. Peters E, Schirris T, van Asbeck AH, Gerretsen J, Eymael J, Ashikov A, Adjobo-Hermans MJ, Russel F, Pickkers P, Masereeuw R. Eur J Pharmacol. 2016 Dec 22;796:149-157. doi: 10.1016/j.ejphar.2016.12.034. PubMed
Deletion of the GAA repeats from the human frataxin gene using the CRISPR-Cas9 system in YG8R-derived cells and mouse models of Friedreich ataxia. Ouellet DL, Cherif K, Rousseau J, Tremblay JP. Gene Ther. 2017 May;24(5):265-274. doi: 10.1038/gt.2016.89. Epub 2016 Dec 26. PubMed
Ibrutinib inhibits pre-BCR+ B-cell acute lymphoblastic leukemia progression by targeting BTK and BLK. Kim E, Hurtz C, Koehrer S, Wang Z, Balasubramanian S, Chang BY, Muschen M, Davis RE, Burger JA. Blood. 2016 Dec 28. pii: blood-2016-06-722900. doi: 10.1182/blood-2016-06-722900. PubMed
Allergen Valency, Dose, and FcepsilonRI Occupancy Set Thresholds for Secretory Responses to Pen a 1 and Motivate Design of Hypoallergens. Mahajan A, Youssef LA, Cleyrat C, Grattan R, Lucero SR, Mattison CP, Erasmus MF, Jacobson B, Tapia L, Hlavacek WS, Schuyler M, Wilson BS. J Immunol. 2016 Dec 30. pii: 1601334. doi: 10.4049/jimmunol.1601334. PubMed
A Model to Investigate Single-Strand DNA Responses in G1 Human Cells via a Telomere-Targeted, Nuclease-Deficient CRISPR-Cas9 System. Crefcoeur RP, Zgheib O, Halazonetis TD. PLoS One. 2017 Jan 3;12(1):e0169126. doi: 10.1371/journal.pone.0169126. eCollection 2017. PubMed
Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides. Rivera-Torres N, Banas K, Bialk P, Bloh KM, Kmiec EB. PLoS One. 2017 Jan 4;12(1):e0169350. doi: 10.1371/journal.pone.0169350. eCollection 2017. PubMed
Genetic disruption of the pHi-regulating proteins Na+/H+ exchanger 1 (SLC9A1) and carbonic anhydrase 9 severely reduces growth of colon cancer cells. Parks SK, Cormerais Y, Durivault J, Pouyssegur J. Oncotarget. 2017 Feb 7;8(6):10225-10237. doi: 10.18632/oncotarget.14379. PubMed
Alternative lengthening of telomeres can be maintained by preferential elongation of lagging strands. Min J, Wright WE, Shay JW. Nucleic Acids Res. 2017 Mar 17;45(5):2615-2628. doi: 10.1093/nar/gkw1295. PubMed
Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion. Phillips AF, Millet AR, Tigano M, Dubois SM, Crimmins H, Babin L, Charpentier M, Piganeau M, Brunet E, Sfeir A. Mol Cell. 2017 Feb 2;65(3):527-538.e6. doi: 10.1016/j.molcel.2016.12.014. Epub 2017 Jan 19. PubMed
Rhesus iPSC Safe Harbor Gene-Editing Platform for Stable Expression of Transgenes in Differentiated Cells of All Germ Layers. Hong SG, Yada RC, Choi K, Carpentier A, Liang TJ, Merling RK, Sweeney CL, Malech HL, Jung M, Corat MAF, AlJanahi AA, Lin Y, Liu H, Tunc I, Wang X, Palisoc M, Pittaluga S, Boehm M, Winkler T, Zou J, Dunbar CE. Mol Ther. 2017 Jan 4;25(1):44-53. doi: 10.1016/j.ymthe.2016.10.007. Epub 2017 Jan 4. PubMed
Histone methyltransferase Setd2 is critical for the proliferation and differentiation of myoblasts. Yi X, Tao Y, Lin X, Dai Y, Yang T, Yue X, Jiang X, Li X, Jiang DS, Andrade KC, Chang J. Biochim Biophys Acta. 2017 Apr;1864(4):697-707. doi: 10.1016/j.bbamcr.2017.01.012. Epub 2017 Jan 24. PubMed
The evolutionary capacitor HSP90 buffers the regulatory effects of mammalian endogenous retroviruses. Hummel B, Hansen EC, Yoveva A, Aprile-Garcia F, Hussong R, Sawarkar R. Nat Struct Mol Biol. 2017 Mar;24(3):234-242. doi: 10.1038/nsmb.3368. Epub 2017 Jan 30. PubMed
Efficient in vivo gene editing using ribonucleoproteins in skin stem cells of recessive dystrophic epidermolysis bullosa mouse model. Wu W, Lu Z, Li F, Wang W, Qian N, Duan J, Zhang Y, Wang F, Chen T. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1660-1665. doi: 10.1073/pnas.1614775114. Epub 2017 Jan 30. PubMed
Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects. Gao Y, Wu H, Wang Y, Liu X, Chen L, Li Q, Cui C, Liu X, Zhang J, Zhang Y. Genome Biol. 2017 Feb 1;18(1):13. doi: 10.1186/s13059-016-1144-4. PubMed
An interaction domain in human SAMD9 is essential for myxoma virus host-range determinant M062 antagonism of host anti-viral function. Nounamo B, Li Y, O'Byrne P, Kearney AM, Khan A, Liu J. Virology. 2017 Mar;503:94-102. doi: 10.1016/j.virol.2017.01.004. Epub 2017 Jan 31. PubMed
Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer. Andretta E, Carton-Garcia F, Martinez-Barriocanal A, de Marcondes PG, Jimenez-Flores LM, Macaya I, Bazzocco S, Bilic J, Rodrigues P, Nieto R, Landolfi S, Ramon Y Cajal S, Schwartz S, Brown A, Dopeso H, Arango D. Sci Rep. 2017 Feb 7;7:41576. doi: 10.1038/srep41576. PubMed
Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Goyal A, Myacheva K, Gross M, Klingenberg M, Duran Arque B, Diederichs S. Nucleic Acids Res. 2017 Feb 17;45(3):e12. doi: 10.1093/nar/gkw883. PubMed
Generation of conditional oncogenic chromosomal translocations using CRISPR-Cas9 genomic editing and homology-directed repair. Spraggon L, Martelotto LG, Hmeljak J, Hitchman TD, Wang J, Wang L, Slotkin EK, Fan PD, Reis-Filho JS, Ladanyi M. J Pathol. 2017 May;242(1):102-112. doi: 10.1002/path.4883. Epub 2017 Mar 30. PubMed
Isoprenylcysteine carboxylmethyltransferase is critical for malignant transformation and tumor maintenance by all RAS isoforms. Lau HY, Tang J, Casey PJ, Wang M. Oncogene. 2017 Jul 6;36(27):3934-3942. doi: 10.1038/onc.2016.508. Epub 2017 Feb 13. PubMed
A fast method for analyzing essential protein mutants in human cells. Dietsch F, Donzeau M, Cordonnier AM, Weiss E, Chatton B, Vigneron M. Biotechniques. 2017 Feb 1;62(2):80-82. doi: 10.2144/000114518. PubMed
Zinc finger protein ZPR9 functions as an activator of AMPK-related serine/threonine kinase MPK38/MELK involved in ASK1/TGF-beta/p53 signaling pathways. Seong HA, Manoharan R, Ha H. Sci Rep. 2017 Feb 14;7:42502. doi: 10.1038/srep42502. PubMed
UV Irradiation Induces a Non-coding RNA that Functionally Opposes the Protein Encoded by the Same Gene. Williamson L, Saponaro M, Boeing S, East P, Mitter R, Kantidakis T, Kelly GP, Lobley A, Walker J, Spencer-Dene B, Howell M, Stewart A, Svejstrup JQ. Cell. 2017 Feb 23;168(5):843-855.e13. doi: 10.1016/j.cell.2017.01.019. Epub 2017 Feb 16. PubMed
Distinct conformations of GPCR-beta-arrestin complexes mediate desensitization, signaling, and endocytosis. Cahill TJ 3rd, Thomsen AR, Tarrasch JT, Plouffe B, Nguyen AH, Yang F, Huang LY, Kahsai AW, Bassoni DL, Gavino BJ, Lamerdin JE, Triest S, Shukla AK, Berger B, Little J 4th, Antar A, Blanc A, Qu CX, Chen X, Kawakami K, Inoue A, Aoki J, Steyaert J, Sun JP, Bouvier M, Skiniotis G, Lefkowitz RJ. Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2562-2567. doi: 10.1073/pnas.1701529114. Epub 2017 Feb 21. PubMed
A PALB2-interacting domain in RNF168 couples homologous recombination to DNA break-induced chromatin ubiquitylation. Luijsterburg MS, Typas D, Caron MC, Wiegant WW, van den Heuvel D, Boonen RA, Couturier AM, Mullenders LH, Masson JY, van Attikum H. Elife. 2017 Feb 27;6. pii: e20922. doi: 10.7554/eLife.20922. PubMed
TIRR regulates 53BP1 by masking its histone methyl-lysine binding function. Drane P, Brault ME, Cui G, Meghani K, Chaubey S, Detappe A, Parnandi N, He Y, Zheng XF, Botuyan MV, Kalousi A, Yewdell WT, Munch C, Harper JW, Chaudhuri J, Soutoglou E, Mer G, Chowdhury D. Nature. 2017 Mar 9;543(7644):211-216. doi: 10.1038/nature21358. Epub 2017 Feb 27. PubMed
YOD1/TRAF6 association balances p62-dependent IL-1 signaling to NF-kappaB. Schimmack G, Schorpp K, Kutzner K, Gehring T, Brenke JK, Hadian K, Krappmann D. Elife. 2017 Feb 28;6. pii: e22416. doi: 10.7554/eLife.22416. PubMed
The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. Lafont E, Kantari-Mimoun C, Draber P, De Miguel D, Hartwig T, Reichert M, Kupka S, Shimizu Y, Taraborrelli L, Spit M, Sprick MR, Walczak H. EMBO J. 2017 May 2;36(9):1147-1166. doi: 10.15252/embj.201695699. Epub 2017 Mar 3. PubMed
Amplified centrosomes and mitotic index display poor concordance between patient tumors and cultured cancer cells. Mittal K, Choi DH, Ogden A, Donthamsetty S, Melton BD, Gupta MV, Pannu V, Cantuaria G, Varambally S, Reid MD, Jonsdottir K, Janssen EA, Aleskandarany MA, Ellis IO, Rakha EA, Rida PC, Aneja R. Sci Rep. 2017 Mar 8;7:43984. doi: 10.1038/srep43984. PubMed
GARLH Family Proteins Stabilize GABAA Receptors at Synapses. Yamasaki T, Hoyos-Ramirez E, Martenson JS, Morimoto-Tomita M, Tomita S. Neuron. 2017 Mar 8;93(5):1138-1152.e6. doi: 10.1016/j.neuron.2017.02.023. PubMed
Purinergic Receptor Transactivation by the beta2-Adrenergic Receptor Increases Intracellular Ca(2+) in Nonexcitable Cells. Stallaert W, van der Westhuizen ET, Schonegge AM, Plouffe B, Hogue M, Lukashova V, Inoue A, Ishida S, Aoki J, Le Gouill C, Bouvier M. Mol Pharmacol. 2017 May;91(5):533-544. doi: 10.1124/mol.116.106419. Epub 2017 Mar 9. PubMed
CRISPR/Cas9-generated p47phox-deficient cell line for Chronic Granulomatous Disease gene therapy vector development. Wrona D, Siler U, Reichenbach J. Sci Rep. 2017 Mar 13;7:44187. doi: 10.1038/srep44187. PubMed
CRISPR system for genome engineering: the application for autophagy study. Cui J, Chew SJL, Shi Y, Gong Z, Shen HM. BMB Rep. 2017 May;50(5):247-256. PubMed
The schizophrenia- and autism-associated gene, transcription factor 4 regulates the columnar distribution of layer 2/3 prefrontal pyramidal neurons in an activity-dependent manner. Page SC, Hamersky GR, Gallo RA, Rannals MD, Calcaterra NE, Campbell MN, Mayfield B, Briley A, Phan BN, Jaffe AE, Maher BJ. Mol Psychiatry. 2018 Feb;23(2):304-315. doi: 10.1038/mp.2017.37. Epub 2017 Mar 14. PubMed
Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation. Jacquin E, Leclerc-Mercier S, Judon C, Blanchard E, Fraitag S, Florey O. Autophagy. 2017 May 4;13(5):854-867. doi: 10.1080/15548627.2017.1287653. Epub 2017 Feb 15. PubMed
Generation of murine tumor cell lines deficient in MHC molecule surface expression using the CRISPR/Cas9 system. Das K, Eisel D, Lenkl C, Goyal A, Diederichs S, Dickes E, Osen W, Eichmuller SB. PLoS One. 2017 Mar 16;12(3):e0174077. doi: 10.1371/journal.pone.0174077. eCollection 2017. PubMed
VEPH1 expression decreases vascularisation in ovarian cancer xenografts and inhibits VEGFA and IL8 expression through inhibition of AKT activation. Shathasivam P, Kollara A, Spybey T, Park S, Clarke B, Ringuette MJ, Brown TJ. Br J Cancer. 2017 Apr 11;116(8):1065-1076. doi: 10.1038/bjc.2017.51. Epub 2017 Mar 16. PubMed
Identification of Significant Pathways Induced by PAX5 Haploinsufficiency Based on Protein-Protein Interaction Networks and Cluster Analysis in Raji Cell Line. Gu J, Li T, Zhao L, Liang X, Fu X, Wang J, Shang Z, Huang W, Zhou J. Biomed Res Int. 2017;2017:5326370. doi: 10.1155/2017/5326370. Epub 2017 Feb 21. PubMed
The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells. Donato V, Bonora M, Simoneschi D, Sartini D, Kudo Y, Saraf A, Florens L, Washburn MP, Stadtfeld M, Pinton P, Pagano M. Nat Cell Biol. 2017 Apr;19(4):341-351. doi: 10.1038/ncb3491. Epub 2017 Mar 20. PubMed
Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9. Xu J, Songyang Z, Liu D, Kim H. Methods Mol Biol. 2017;1587:15-28. doi: 10.1007/978-1-4939-6892-3_2. PubMed
CRISPR-Cas9-guided oncogenic chromosomal translocations with conditional fusion protein expression in human mesenchymal cells. Vanoli F, Tomishima M, Feng W, Lamribet K, Babin L, Brunet E, Jasin M. Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):3696-3701. doi: 10.1073/pnas.1700622114. Epub 2017 Mar 21. PubMed
FMNL formins boost lamellipodial force generation. Kage F, Winterhoff M, Dimchev V, Mueller J, Thalheim T, Freise A, Bruhmann S, Kollasser J, Block J, Dimchev G, Geyer M, Schnittler HJ, Brakebusch C, Stradal TE, Carlier MF, Sixt M, Kas J, Faix J, Rottner K. Nat Commun. 2017 Mar 22;8:14832. doi: 10.1038/ncomms14832. PubMed
A platform for functional assessment of large variant libraries in mammalian cells. Matreyek KA, Stephany JJ, Fowler DM. Nucleic Acids Res. 2017 Mar 15. doi: 10.1093/nar/gkx183. PubMed
Src family kinases Fyn and Lyn are constitutively activated and mediate plasmacytoid dendritic cell responses. Dallari S, Macal M, Loureiro ME, Jo Y, Swanson L, Hesser C, Ghosh P, Zuniga EI. Nat Commun. 2017 Apr 3;8:14830. doi: 10.1038/ncomms14830. PubMed
Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette exchange. Kelton W, Waindok AC, Pesch T, Pogson M, Ford K, Parola C, Reddy ST. Sci Rep. 2017 Apr 4;7:45775. doi: 10.1038/srep45775. PubMed

Associated Plasmids

A Ca2+ channel differentially regulates Clathrin-mediated and activity-dependent bulk endocytosis. Yao CK, Liu YT, Lee IC, Wang YT, Wu PY. PLoS Biol. 2017 Apr 17;15(4):e2000931. doi: 10.1371/journal.pbio.2000931. eCollection 2017 Apr. PubMed
PGE2/EP3/SRC signaling induces EGFR nuclear translocation and growth through EGFR ligands release in lung adenocarcinoma cells. Bazzani L, Donnini S, Finetti F, Christofori G, Ziche M. Oncotarget. 2017 May 9;8(19):31270-31287. doi: 10.18632/oncotarget.16116. PubMed
TIAM1 Antagonizes TAZ/YAP Both in the Destruction Complex in the Cytoplasm and in the Nucleus to Inhibit Invasion of Intestinal Epithelial Cells. Diamantopoulou Z, White G, Fadlullah MZH, Dreger M, Pickering K, Maltas J, Ashton G, MacLeod R, Baillie GS, Kouskoff V, Lacaud G, Murray GI, Sansom OJ, Hurlstone AFL, Malliri A. Cancer Cell. 2017 May 8;31(5):621-634.e6. doi: 10.1016/j.ccell.2017.03.007. Epub 2017 Apr 13. PubMed
CRISPR-Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice. Zhang Y, Long C, Li H, McAnally JR, Baskin KK, Shelton JM, Bassel-Duby R, Olson EN. Sci Adv. 2017 Apr 12;3(4):e1602814. doi: 10.1126/sciadv.1602814. eCollection 2017 Apr. PubMed
beta2-Adrenergic receptor activation mobilizes intracellular calcium via a non-canonical cAMP-independent signaling pathway. Galaz-Montoya M, Wright SJ, Rodriguez GJ, Lichtarge O, Wensel TG. J Biol Chem. 2017 Jun 16;292(24):9967-9974. doi: 10.1074/jbc.M117.787119. Epub 2017 Apr 25. PubMed
Human phosphatase CDC14A regulates actin organization through dephosphorylation of epithelial protein lost in neoplasm. Chen NP, Uddin B, Hardt R, Ding W, Panic M, Lucibello I, Kammerer P, Ruppert T, Schiebel E. Proc Natl Acad Sci U S A. 2017 May 16;114(20):5201-5206. doi: 10.1073/pnas.1619356114. Epub 2017 May 2. PubMed
Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9. Higashioka K, Koizumi N, Sakurai H, Sotozono C, Sato T. Stem Cells Int. 2017;2017:9210494. doi: 10.1155/2017/9210494. Epub 2017 Apr 4. PubMed
Complement factor P is a ligand for the natural killer cell-activating receptor NKp46. Narni-Mancinelli E, Gauthier L, Baratin M, Guia S, Fenis A, Deghmane AE, Rossi B, Fourquet P, Escaliere B, Kerdiles YM, Ugolini S, Taha MK, Vivier E. Sci Immunol. 2017 Apr 28;2(10). pii: eaam9628. doi: 10.1126/sciimmunol.aam9628. PubMed
Genetic and pharmacological antagonism of NK1 receptor prevents opiate abuse potential. Sandweiss AJ, McIntosh MI, Moutal A, Davidson-Knapp R, Hu J, Giri AK, Yamamoto T, Hruby VJ, Khanna R, Largent-Milnes TM, Vanderah TW. Mol Psychiatry. 2018 Aug;23(8):1745-1755. doi: 10.1038/mp.2017.102. Epub 2017 May 9. PubMed
A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma. Tammela T, Sanchez-Rivera FJ, Cetinbas NM, Wu K, Joshi NS, Helenius K, Park Y, Azimi R, Kerper NR, Wesselhoeft RA, Gu X, Schmidt L, Cornwall-Brady M, Yilmaz OH, Xue W, Katajisto P, Bhutkar A, Jacks T. Nature. 2017 May 18;545(7654):355-359. doi: 10.1038/nature22334. Epub 2017 May 10. PubMed
Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity. Bednash JS, Weathington N, Londino J, Rojas M, Gulick DL, Fort R, Han S, McKelvey AC, Chen BB, Mallampalli RK. Nat Commun. 2017 May 11;8:15203. doi: 10.1038/ncomms15203. PubMed
Lack of MTTP Activity in Pluripotent Stem Cell-Derived Hepatocytes and Cardiomyocytes Abolishes apoB Secretion and Increases Cell Stress. Liu Y, Conlon DM, Bi X, Slovik KJ, Shi J, Edelstein HI, Millar JS, Javaheri A, Cuchel M, Pashos EE, Iqbal J, Hussain MM, Hegele RA, Yang W, Duncan SA, Rader DJ, Morrisey EE. Cell Rep. 2017 May 16;19(7):1456-1466. doi: 10.1016/j.celrep.2017.04.064. PubMed
LDB1-mediated enhancer looping can be established independent of mediator and cohesin. Krivega I, Dean A. Nucleic Acids Res. 2017 May 18. doi: 10.1093/nar/gkx433. PubMed
Generation of chromosomal translocations that lead to conditional fusion protein expression using CRISPR-Cas9 and homology-directed repair. Vanoli F, Jasin M. Methods. 2017 May 15;121-122:138-145. doi: 10.1016/j.ymeth.2017.05.006. Epub 2017 May 15. PubMed
Dynamic regulation of VEGF-inducible genes by an ERK/ERG/p300 transcriptional network. Fish JE, Cantu Gutierrez M, Dang LT, Khyzha N, Chen Z, Veitch S, Cheng HS, Khor M, Antounians L, Njock MS, Boudreau E, Herman AM, Rhyner AM, Ruiz OE, Eisenhoffer GT, Medina-Rivera A, Wilson MD, Wythe JD. Development. 2017 Jul 1;144(13):2428-2444. doi: 10.1242/dev.146050. Epub 2017 May 23. PubMed
CD7-edited T cells expressing a CD7-specific CAR for the therapy of T-cell malignancies. Gomes-Silva D, Srinivasan M, Sharma S, Lee CM, Wagner DL, Davis TH, Rouce RH, Bao G, Brenner MK, Mamonkin M. Blood. 2017 Jul 20;130(3):285-296. doi: 10.1182/blood-2017-01-761320. Epub 2017 May 24. PubMed
Reduced Expression of Foxp1 as a Contributing Factor in Huntington's Disease. Louis Sam Titus ASC, Yusuff T, Cassar M, Thomas E, Kretzschmar D, D'Mello SR. J Neurosci. 2017 Jul 5;37(27):6575-6587. doi: 10.1523/JNEUROSCI.3612-16.2017. Epub 2017 May 26. PubMed
Efficient generation of targeted and controlled mutational events in porcine cells using nuclease-directed homologous recombination. Butler JR, Santos RMN, Martens GR, Ladowski JM, Wang ZY, Li P, Tector M, Tector AJ. J Surg Res. 2017 May 15;212:238-245. doi: 10.1016/j.jss.2017.01.025. Epub 2017 Jan 29. PubMed
Biallelic Mutations in CFAP43 and CFAP44 Cause Male Infertility with Multiple Morphological Abnormalities of the Sperm Flagella. Tang S, Wang X, Li W, Yang X, Li Z, Liu W, Li C, Zhu Z, Wang L, Wang J, Zhang L, Sun X, Zhi E, Wang H, Li H, Jin L, Luo Y, Wang J, Yang S, Zhang F. Am J Hum Genet. 2017 Jun 1;100(6):854-864. doi: 10.1016/j.ajhg.2017.04.012. Epub 2017 May 25. PubMed
Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy. Li X, Yu W, Qian X, Xia Y, Zheng Y, Lee JH, Li W, Lyu J, Rao G, Zhang X, Qian CN, Rozen SG, Jiang T, Lu Z. Mol Cell. 2017 Jun 1;66(5):684-697.e9. doi: 10.1016/j.molcel.2017.04.026. Epub 2017 May 25. PubMed
Biallelic TRIP13 mutations predispose to Wilms tumor and chromosome missegregation. Yost S, de Wolf B, Hanks S, Zachariou A, Marcozzi C, Clarke M, de Voer R, Etemad B, Uijttewaal E, Ramsay E, Wylie H, Elliott A, Picton S, Smith A, Smithson S, Seal S, Ruark E, Houge G, Pines J, Kops GJPL, Rahman N. Nat Genet. 2017 Jul;49(7):1148-1151. doi: 10.1038/ng.3883. Epub 2017 May 29. PubMed
Myomerger induces fusion of non-fusogenic cells and is required for skeletal muscle development. Quinn ME, Goh Q, Kurosaka M, Gamage DG, Petrany MJ, Prasad V, Millay DP. Nat Commun. 2017 Jun 1;8:15665. doi: 10.1038/ncomms15665. PubMed
SCYL1 does not regulate REST expression and turnover. Gingras S, Kuliyev E, Pelletier S. PLoS One. 2017 Jun 1;12(6):e0178680. doi: 10.1371/journal.pone.0178680. eCollection 2017. PubMed
Low expression of PinX1 is associated with malignant behavior in basal-like breast cancer. Feng YZ, Zhang QY, Fu MT, Zhang ZF, Wei M, Zhou JY, Shi R. Oncol Rep. 2017 Jul;38(1):109-119. doi: 10.3892/or.2017.5696. Epub 2017 Jun 2. PubMed
Intercellular transmission of the unfolded protein response promotes survival and drug resistance in cancer cells. Rodvold JJ, Chiu KT, Hiramatsu N, Nussbacher JK, Galimberti V, Mahadevan NR, Willert K, Lin JH, Zanetti M. Sci Signal. 2017 Jun 6;10(482). pii: 10/482/eaah7177. doi: 10.1126/scisignal.aah7177. PubMed
The beneficial metabolic effects of insulin sensitizers are not attenuated by mitochondrial pyruvate carrier 2 hypomorphism. Vigueira PA, McCommis KS, Hodges WT, Schweitzer GG, Cole SL, Oonthonpan L, Taylor EB, McDonald WG, Kletzien RF, Colca JR, Finck BN. Exp Physiol. 2017 Jun 9. doi: 10.1113/EP086380. PubMed
Mutation of nucleotides around the +1 position of type 3 polymerase III promoters: The effect on transcriptional activity and start site usage. Gao Z, Harwig A, Berkhout B, Herrera-Carrillo E. Transcription. 2017 Jun 9:e1322170. doi: 10.1080/21541264.2017.1322170. PubMed
Reduction of infection by inhibiting mTOR pathway is associated with reversed repression of type I interferon by porcine reproductive and respiratory syndrome virus. Liu Q, Miller LC, Blecha F, Sang Y. J Gen Virol. 2017 Jun;98(6):1316-1328. doi: 10.1099/jgv.0.000802. Epub 2017 Jun 14. PubMed
PTEN counteracts FBXL2 to promote IP3R3- and Ca(2+)-mediated apoptosis limiting tumour growth. Kuchay S, Giorgi C, Simoneschi D, Pagan J, Missiroli S, Saraf A, Florens L, Washburn MP, Collazo-Lorduy A, Castillo-Martin M, Cordon-Cardo C, Sebti SM, Pinton P, Pagano M. Nature. 2017 Jun 22;546(7659):554-558. doi: 10.1038/nature22965. Epub 2017 Jun 14. PubMed
CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome. Bloh KM, Bialk PA, Gopalakrishnapillai A, Kolb EA, Kmiec EB. Mol Ther Nucleic Acids. 2017 Jun 16;7:288-298. doi: 10.1016/j.omtn.2017.04.009. Epub 2017 Apr 13. PubMed
Extensive Proliferation of Human Cancer Cells with Ever-Shorter Telomeres. Dagg RA, Pickett HA, Neumann AA, Napier CE, Henson JD, Teber ET, Arthur JW, Reynolds CP, Murray J, Haber M, Sobinoff AP, Lau LMS, Reddel RR. Cell Rep. 2017 Jun 20;19(12):2544-2556. doi: 10.1016/j.celrep.2017.05.087. PubMed
Non-canonical Glucocorticoid Receptor Transactivation of gilz by Alcohol Suppresses Cell Inflammatory Response. Ng HP, Jennings S, Wang J, Molina PE, Nelson S, Wang G. Front Immunol. 2017 Jun 7;8:661. doi: 10.3389/fimmu.2017.00661. eCollection 2017. PubMed
Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells. Hindriksen S, Bramer AJ, Truong MA, Vromans MJM, Post JB, Verlaan-Klink I, Snippert HJ, Lens SMA, Hadders MA. PLoS One. 2017 Jun 22;12(6):e0179514. doi: 10.1371/journal.pone.0179514. eCollection 2017. PubMed
KLF1 drives the expression of fetal hemoglobin in British HPFH. Wienert B, Martyn GE, Kurita R, Nakamura Y, Quinlan KGR, Crossley M. Blood. 2017 Jun 28. pii: blood-2017-02-767400. doi: 10.1182/blood-2017-02-767400. PubMed
METTL21B is a novel human lysine methyltransferase of translation elongation factor 1A: discovery by CRISPR/Cas9 knock out. Hamey JJ, Wienert B, Quinlan KGR, Wilkins MR. Mol Cell Proteomics. 2017 Jun 29. pii: mcp.M116.066308. doi: 10.1074/mcp.M116.066308. PubMed
Viral Replication Complexes Are Targeted by LC3-Guided Interferon-Inducible GTPases. Biering SB, Choi J, Halstrom RA, Brown HM, Beatty WL, Lee S, McCune BT, Dominici E, Williams LE, Orchard RC, Wilen CB, Yamamoto M, Coers J, Taylor GA, Hwang S. Cell Host Microbe. 2017 Jul 12;22(1):74-85.e7. doi: 10.1016/j.chom.2017.06.005. Epub 2017 Jun 29. PubMed
Generation and characterization of a human iPSC cell line expressing inducible Cas9 in the "safe harbor" AAVS1 locus. Castano J, Bueno C, Jimenez-Delgado S, Roca-Ho H, Fraga MF, Fernandez AF, Nakanishi M, Torres-Ruiz R, Rodriguez-Perales S, Menendez P. Stem Cell Res. 2017 May;21:137-140. doi: 10.1016/j.scr.2017.04.011. Epub 2017 Apr 22. PubMed
Generation of a Bag1 homozygous knockout mouse embryonic stem cell line using CRISPR/Cas9. Tang CC, Shan LP, Wang WM, Lu G, Tare RS, Lee KKH. Stem Cell Res. 2017 May;21:29-31. doi: 10.1016/j.scr.2017.03.016. Epub 2017 Mar 23. PubMed
The host E3-Ubiquitin ligase TRIM6 ubiquitinates the Ebola virus VP35 protein and promotes virus replication. Bharaj P, Atkins C, Luthra P, Giraldo MI, Dawes BE, Miorin L, Johnson JR, Krogan NJ, Basler CF, Freiberg AN, Rajsbaum R. J Virol. 2017 Jul 5. pii: JVI.00833-17. doi: 10.1128/JVI.00833-17. PubMed
A Type 2 Diabetes-Associated Functional Regulatory Variant in a Pancreatic Islet Enhancer at the Adcy5 Locus. Roman TS, Cannon ME, Vadlamudi S, Buchkovich ML, Wolford BN, Welch RP, Morken MA, Kwon GJ, Varshney A, Kursawe R, Wu Y, Jackson AU, Erdos MR, Kuusisto J, Laakso M, Scott LJ, Boehnke M, Collins FS, Parker SCJ, Stitzel ML, Mohlke KL. Diabetes. 2017 Jul 6. pii: db170464. doi: 10.2337/db17-0464. PubMed
Inactivation of Pol theta and C-NHEJ eliminates off-target integration of exogenous DNA. Zelensky AN, Schimmel J, Kool H, Kanaar R, Tijsterman M. Nat Commun. 2017 Jul 7;8(1):66. doi: 10.1038/s41467-017-00124-3. PubMed
Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Flack JE, Mieszczanek J, Novcic N, Bienz M. Mol Cell. 2017 Jul 20;67(2):181-193.e5. doi: 10.1016/j.molcel.2017.06.009. Epub 2017 Jul 6. PubMed
The Guanine-Nucleotide Exchange Factor Caldag Gefi Fine-Tunes Functional Properties of Regulatory T Cells. Niemz J, Kliche S, Pils MC, Morrison E, Manns A, Freund C, Crittenden JR, Graybiel AM, Galla M, Jansch L, Huehn J. Eur J Microbiol Immunol (Bp). 2017 May 22;7(2):112-126. doi: 10.1556/1886.2017.00007. eCollection 2017 Jun. PubMed
Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein. Lei Y, Zhang X, Su J, Jeong M, Gundry MC, Huang YH, Zhou Y, Li W, Goodell MA. Nat Commun. 2017 Jul 11;8:16026. doi: 10.1038/ncomms16026. PubMed

Associated Plasmids

tsRNA signatures in cancer. Balatti V, Nigita G, Veneziano D, Drusco A, Stein GS, Messier TL, Farina NH, Lian JB, Tomasello L, Liu CG, Palamarchuk A, Hart JR, Bell C, Carosi M, Pescarmona E, Perracchio L, Diodoro M, Russo A, Antenucci A, Visca P, Ciardi A, Harris CC, Vogt PK, Pekarsky Y, Croce CM. Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):8071-8076. doi: 10.1073/pnas.1706908114. Epub 2017 Jul 10. PubMed
Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer. Nabet BY, Qiu Y, Shabason JE, Wu TJ, Yoon T, Kim BC, Benci JL, DeMichele AM, Tchou J, Marcotrigiano J, Minn AJ. Cell. 2017 Jul 13;170(2):352-366.e13. doi: 10.1016/j.cell.2017.06.031. PubMed
The ciliary membrane-associated proteome reveals actin-binding proteins as key components of cilia. Kohli P, Hohne M, Jungst C, Bertsch S, Ebert LK, Schauss AC, Benzing T, Rinschen MM, Schermer B. EMBO Rep. 2017 Sep;18(9):1521-1535. doi: 10.15252/embr.201643846. Epub 2017 Jul 14. PubMed
Cytosolic interaction of type III human CD38 with CIB1 modulates cellular cyclic ADP-ribose levels. Liu J, Zhao YJ, Li WH, Hou YN, Li T, Zhao ZY, Fang C, Li SL, Lee HC. Proc Natl Acad Sci U S A. 2017 Jul 18. pii: 1703718114. doi: 10.1073/pnas.1703718114. PubMed
Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK. Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, Lin SC. Nature. 2017 Aug 3;548(7665):112-116. doi: 10.1038/nature23275. Epub 2017 Jul 19. PubMed
Histone demethylase PHF8 regulates hypoxia signaling through HIF1alpha and H3K4me3. Maina PK, Shao P, Jia X, Liu Q, Umesalma S, Marin M, Long D Jr, Concepcion-Roman S, Qi HH. Biochim Biophys Acta Gene Regul Mech. 2017 Sep;1860(9):1002-1012. doi: 10.1016/j.bbagrm.2017.07.005. Epub 2017 Jul 20. PubMed
Argonaute CLIP Defines a Deregulated miR-122-Bound Transcriptome that Correlates with Patient Survival in Human Liver Cancer. Luna JM, Barajas JM, Teng KY, Sun HL, Moore MJ, Rice CM, Darnell RB, Ghoshal K. Mol Cell. 2017 Aug 3;67(3):400-410.e7. doi: 10.1016/j.molcel.2017.06.025. Epub 2017 Jul 20. PubMed
DDX6 Represses Aberrant Activation of Interferon-Stimulated Genes. Lumb JH, Li Q, Popov LM, Ding S, Keith MT, Merrill BD, Greenberg HB, Li JB, Carette JE. Cell Rep. 2017 Jul 25;20(4):819-831. doi: 10.1016/j.celrep.2017.06.085. PubMed
A biallelic mutation in IL6ST encoding the GP130 co-receptor causes immunodeficiency and craniosynostosis. Schwerd T, Twigg SRF, Aschenbrenner D, Manrique S, Miller KA, Taylor IB, Capitani M, McGowan SJ, Sweeney E, Weber A, Chen L, Bowness P, Riordan A, Cant A, Freeman AF, Milner JD, Holland SM, Frede N, Muller M, Schmidt-Arras D, Grimbacher B, Wall SA, Jones EY, Wilkie AOM, Uhlig HH. J Exp Med. 2017 Sep 4;214(9):2547-2562. doi: 10.1084/jem.20161810. Epub 2017 Jul 26. PubMed
A genome-wide CRISPR screen reconciles the role of N-linked glycosylation in galectin-3 transport to the cell surface. Stewart SE, Menzies SA, Popa SJ, Savinykh N, Petrunkina Harrison A, Lehner PJ, Moreau K. J Cell Sci. 2017 Oct 1;130(19):3234-3247. doi: 10.1242/jcs.206425. Epub 2017 Aug 3. PubMed
The Sharpin interactome reveals a role for Sharpin in lamellipodium formation via the Arp2/3 complex. Khan MH, Salomaa SI, Jacquemet G, Butt U, Miihkinen M, Deguchi T, Kremneva E, Lappalainen P, Humphries MJ, Pouwels J. J Cell Sci. 2017 Sep 15;130(18):3094-3107. doi: 10.1242/jcs.200329. Epub 2017 Aug 3. PubMed
Fibroblast growth factor receptor is a mechanistic link between visceral adiposity and cancer. Chakraborty D, Benham V, Bullard B, Kearney T, Hsia HC, Gibbon D, Demireva EY, Lunt SY, Bernard JJ. Oncogene. 2017 Nov 30;36(48):6668-6679. doi: 10.1038/onc.2017.278. Epub 2017 Aug 7. PubMed
Complete Acid Ceramidase ablation prevents cancer-initiating cell formation in melanoma cells. Lai M, Realini N, La Ferla M, Passalacqua I, Matteoli G, Ganesan A, Pistello M, Mazzanti CM, Piomelli D. Sci Rep. 2017 Aug 7;7(1):7411. doi: 10.1038/s41598-017-07606-w. PubMed
A pluripotent stem cell-based model for post-implantation human amniotic sac development. Shao Y, Taniguchi K, Townshend RF, Miki T, Gumucio DL, Fu J. Nat Commun. 2017 Aug 8;8(1):208. doi: 10.1038/s41467-017-00236-w. PubMed
Antibody-mediated blockade of JMJD6 interaction with collagen I exerts antifibrotic and antimetastatic activities. Miotti S, Gulino A, Ferri R, Parenza M, Chronowska A, Lecis D, Sangaletti S, Tagliabue E, Tripodo C, Colombo MP. FASEB J. 2017 Dec;31(12):5356-5370. doi: 10.1096/fj.201700377R. Epub 2017 Aug 8. PubMed
Identification and characterization of a long non-coding RNA up-regulated during HIV-1 infection. Postler TS, Pantry SN, Desrosiers RC, Ghosh S. Virology. 2017 Nov;511:30-39. doi: 10.1016/j.virol.2017.08.006. Epub 2017 Aug 10. PubMed
Improved androgen specificity of AR-EcoScreen by CRISPR based glucocorticoid receptor knockout. Zwart N, Andringa D, de Leeuw WJ, Kojima H, Iida M, Houtman CJ, de Boer J, Kool J, Lamoree MH, Hamers T. Toxicol In Vitro. 2017 Dec;45(Pt 1):1-9. doi: 10.1016/j.tiv.2017.08.004. Epub 2017 Aug 11. PubMed
CRISPR/Cas9 editing of Nf1 gene identifies CRMP2 as a therapeutic target in neurofibromatosis type 1-related pain that is reversed by (S)-Lacosamide. Moutal A, Yang X, Li W, Gilbraith KB, Luo S, Cai S, Francois-Moutal L, Chew LA, Yeon SK, Bellampalli SS, Qu C, Xie JY, Ibrahim MM, Khanna M, Park KD, Porreca F, Khanna R. Pain. 2017 Dec;158(12):2301-2319. doi: 10.1097/j.pain.0000000000001002. PubMed
BORC coordinates encounter and fusion of lysosomes with autophagosomes. Jia R, Guardia CM, Pu J, Chen Y, Bonifacino JS. Autophagy. 2017 Oct 3;13(10):1648-1663. doi: 10.1080/15548627.2017.1343768. Epub 2017 Aug 21. PubMed
Inactivation of CREBBP expands the germinal center B cell compartment, down-regulates MHCII expression and promotes DLBCL growth. Hashwah H, Schmid CA, Kasser S, Bertram K, Stelling A, Manz MG, Muller A. Proc Natl Acad Sci U S A. 2017 Sep 5;114(36):9701-9706. doi: 10.1073/pnas.1619555114. Epub 2017 Aug 22. PubMed
The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress alpha-synuclein aggregation. Aprile FA, Kallstig E, Limorenko G, Vendruscolo M, Ron D, Hansen C. Sci Rep. 2017 Aug 22;7(1):9039. doi: 10.1038/s41598-017-08324-z. PubMed
Pseudomonas aeruginosa ExlA and Serratia marcescens ShlA trigger cadherin cleavage by promoting calcium influx and ADAM10 activation. Reboud E, Bouillot S, Patot S, Beganton B, Attree I, Huber P. PLoS Pathog. 2017 Aug 23;13(8):e1006579. doi: 10.1371/journal.ppat.1006579. eCollection 2017 Aug. PubMed
Palmitoylation of the ciliary GTPase ARL13b is necessary for its stability and its role in cilia formation. Roy K, Jerman S, Jozsef L, McNamara T, Onyekaba G, Sun Z, Marin EP. J Biol Chem. 2017 Oct 27;292(43):17703-17717. doi: 10.1074/jbc.M117.792937. Epub 2017 Aug 28. PubMed
Differential mast cell outcomes are sensitive to FcepsilonRI-Syk binding kinetics. Schwartz SL, Cleyrat C, Olah MJ, Relich PK, Phillips GK, Hlavacek WS, Lidke KA, Wilson BS, Lidke DS. Mol Biol Cell. 2017 Nov 7;28(23):3397-3414. doi: 10.1091/mbc.E17-06-0350. Epub 2017 Aug 30. PubMed
Editing an alpha-globin enhancer in primary human hematopoietic stem cells as a treatment for beta-thalassemia. Mettananda S, Fisher CA, Hay D, Badat M, Quek L, Clark K, Hublitz P, Downes D, Kerry J, Gosden M, Telenius J, Sloane-Stanley JA, Faustino P, Coelho A, Doondeea J, Usukhbayar B, Sopp P, Sharpe JA, Hughes JR, Vyas P, Gibbons RJ, Higgs DR. Nat Commun. 2017 Sep 4;8(1):424. doi: 10.1038/s41467-017-00479-7. PubMed
miR-33a inhibits cell proliferation and invasion by targeting CAND1 in lung cancer. Kang M, Li Y, Zhao Y, He S, Shi J. Clin Transl Oncol. 2018 Apr;20(4):457-466. doi: 10.1007/s12094-017-1730-2. Epub 2017 Sep 4. PubMed
Mitochondrial fission facilitates the selective mitophagy of protein aggregates. Burman JL, Pickles S, Wang C, Sekine S, Vargas JNS, Zhang Z, Youle AM, Nezich CL, Wu X, Hammer JA, Youle RJ. J Cell Biol. 2017 Oct 2;216(10):3231-3247. doi: 10.1083/jcb.201612106. Epub 2017 Sep 11. PubMed
SOCS1 is an inducible negative regulator of interferon lambda (IFN-lambda)-induced gene expression in vivo. Blumer T, Coto-Llerena M, Duong FHT, Heim MH. J Biol Chem. 2017 Oct 27;292(43):17928-17938. doi: 10.1074/jbc.M117.788877. Epub 2017 Sep 12. PubMed
Autophagy-Dependent Generation of Free Fatty Acids Is Critical for Normal Neutrophil Differentiation. Riffelmacher T, Clarke A, Richter FC, Stranks A, Pandey S, Danielli S, Hublitz P, Yu Z, Johnson E, Schwerd T, McCullagh J, Uhlig H, Jacobsen SEW, Simon AK. Immunity. 2017 Sep 19;47(3):466-480.e5. doi: 10.1016/j.immuni.2017.08.005. Epub 2017 Sep 12. PubMed
Apoptotic Bodies Elicit Gas6-Mediated Migration of AXL-Expressing Tumor Cells. Zweemer AJM, French CB, Mesfin J, Gordonov S, Meyer AS, Lauffenburger DA. Mol Cancer Res. 2017 Dec;15(12):1656-1666. doi: 10.1158/1541-7786.MCR-17-0012. Epub 2017 Sep 18. PubMed
MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Moreno-Fortuny A, Bragg L, Cossu G, Roostalu U. Biol Open. 2017 Nov 15;6(11):1592-1601. doi: 10.1242/bio.027771. PubMed
Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells. Chung CY, Wang Q, Yang S, Ponce SA, Kirsch BJ, Zhang H, Betenbaugh MJ. Biotechnol Bioeng. 2017 Dec;114(12):2848-2856. doi: 10.1002/bit.26375. Epub 2017 Sep 19. PubMed
Functional correction of dystrophin actin binding domain mutations by genome editing. Kyrychenko V, Kyrychenko S, Tiburcy M, Shelton JM, Long C, Schneider JW, Zimmermann WH, Bassel-Duby R, Olson EN. JCI Insight. 2017 Sep 21;2(18). pii: 95918. doi: 10.1172/jci.insight.95918. eCollection 2017 Sep 21. PubMed
The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma. Mroczek S, Chlebowska J, Kulinski TM, Gewartowska O, Gruchota J, Cysewski D, Liudkovska V, Borsuk E, Nowis D, Dziembowski A. Nat Commun. 2017 Sep 20;8(1):619. doi: 10.1038/s41467-017-00578-5. PubMed
HGFL-mediated RON signaling supports breast cancer stem cell phenotypes via activation of non-canonical beta-catenin signaling. Ruiz-Torres SJ, Benight NM, Karns RA, Lower EE, Guan JL, Waltz SE. Oncotarget. 2017 Jul 22;8(35):58918-58933. doi: 10.18632/oncotarget.19441. eCollection 2017 Aug 29. PubMed
Cic Loss Promotes Gliomagenesis via Aberrant Neural Stem Cell Proliferation and Differentiation. Yang R, Chen LH, Hansen LJ, Carpenter AB, Moure CJ, Liu H, Pirozzi CJ, Diplas BH, Waitkus MS, Greer PK, Zhu H, McLendon RE, Bigner DD, He Y, Yan H. Cancer Res. 2017 Nov 15;77(22):6097-6108. doi: 10.1158/0008-5472.CAN-17-1018. Epub 2017 Sep 22. PubMed
Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers. Yau EH, Kummetha IR, Lichinchi G, Tang R, Zhang Y, Rana TM. Cancer Res. 2017 Nov 15;77(22):6330-6339. doi: 10.1158/0008-5472.CAN-17-2043. Epub 2017 Sep 27. PubMed
Diminished PRRX1 Expression Is Associated With Increased Risk of Atrial Fibrillation and Shortening of the Cardiac Action Potential. Tucker NR, Dolmatova EV, Lin H, Cooper RR, Ye J, Hucker WJ, Jameson HS, Parsons VA, Weng LC, Mills RW, Sinner MF, Imakaev M, Leyton-Mange J, Vlahakes G, Benjamin EJ, Lunetta KL, Lubitz SA, Mirny L, Milan DJ, Ellinor PT. Circ Cardiovasc Genet. 2017 Oct;10(5). pii: CIRCGENETICS.117.001902. doi: 10.1161/CIRCGENETICS.117.001902. PubMed
Enhanced Genome Editing Tools For Multi-Gene Deletion Knock-Out Approaches Using Paired CRISPR sgRNAs in CHO Cells. Schmieder V, Bydlinski N, Strasser R, Baumann M, Kildegaard HF, Jadhav V, Borth N. Biotechnol J. 2018 Mar;13(3):e1700211. doi: 10.1002/biot.201700211. Epub 2017 Nov 13. PubMed
TRPC5 is required for the NO-dependent increase in dendritic Ca(2+) and GABA release from chick retinal amacrine cells. Maddox JW, Khorsandi N, Gleason E. J Neurophysiol. 2018 Jan 1;119(1):262-273. doi: 10.1152/jn.00500.2017. Epub 2017 Oct 4. PubMed
Distinct Cell-Cycle Control in Two Different States of Mouse Pluripotency. Ter Huurne M, Chappell J, Dalton S, Stunnenberg HG. Cell Stem Cell. 2017 Oct 5;21(4):449-455.e4. doi: 10.1016/j.stem.2017.09.004. PubMed
Phosphorylation of Threonine(343) Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency. Abulaiti X, Zhang H, Wang A, Li N, Li Y, Wang C, Du X, Li L. Stem Cell Reports. 2017 Nov 14;9(5):1630-1641. doi: 10.1016/j.stemcr.2017.09.001. Epub 2017 Oct 5. PubMed
A Ragulator-BORC interaction controls lysosome positioning in response to amino acid availability. Pu J, Keren-Kaplan T, Bonifacino JS. J Cell Biol. 2017 Dec 4;216(12):4183-4197. doi: 10.1083/jcb.201703094. Epub 2017 Oct 9. PubMed
CDK9-dependent RNA polymerase II pausing controls transcription initiation. Gressel S, Schwalb B, Decker TM, Qin W, Leonhardt H, Eick D, Cramer P. Elife. 2017 Oct 10;6. pii: 29736. doi: 10.7554/eLife.29736. PubMed
Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters. Chen Y, Spitzer S, Agathou S, Karadottir RT, Smith A. Stem Cell Reports. 2017 Oct 10;9(4):1262-1274. doi: 10.1016/j.stemcr.2017.09.005. PubMed
A point mutation in the extracellular domain of CD4 completely abolishes CD4 T cell development in C57BL/6 mouse. Wang H, Li S, Chao T, Wang X, Shi L, Zhang L, Liang Y, Zheng Q, Lu L. Mol Immunol. 2017 Dec;92:12-20. doi: 10.1016/j.molimm.2017.09.015. Epub 2017 Oct 10. PubMed
Characterization and use of the novel human multiple myeloma cell line MC-B11/14 to study biological consequences of CRISPR-mediated loss of immunoglobulin A heavy chain. Walters DK, Arendt BK, Tschumper RC, Wu X, Jelinek DF. Exp Hematol. 2018 Jan;57:42-49.e1. doi: 10.1016/j.exphem.2017.09.010. Epub 2017 Oct 13. PubMed
Viperin Targets Flavivirus Virulence by Inducing Assembly of Noninfectious Capsid Particles. Vonderstein K, Nilsson E, Hubel P, Nygard Skalman L, Upadhyay A, Pasto J, Pichlmair A, Lundmark R, Overby AK. J Virol. 2017 Dec 14;92(1). pii: JVI.01751-17. doi: 10.1128/JVI.01751-17. Print 2018 Jan 1. PubMed
Characterization of gamma-tubulin filaments in mammalian cells. Lindstrom L, Alvarado-Kristensson M. Biochim Biophys Acta. 2017 Oct 16. pii: S0167-4889(17)30283-5. doi: 10.1016/j.bbamcr.2017.10.008. PubMed

Associated Plasmids

UNC-45a promotes myosin folding and stress fiber assembly. Lehtimaki JI, Fenix AM, Kotila TM, Balistreri G, Paavolainen L, Varjosalo M, Burnette DT, Lappalainen P. J Cell Biol. 2017 Dec 4;216(12):4053-4072. doi: 10.1083/jcb.201703107. Epub 2017 Oct 20. PubMed
A cautionary tale of sense-antisense gene pairs: independent regulation despite inverse correlation of expression. Goyal A, Fiskin E, Gutschner T, Polycarpou-Schwarz M, Gross M, Neugebauer J, Gandhi M, Caudron-Herger M, Benes V, Diederichs S. Nucleic Acids Res. 2017 Dec 1;45(21):12496-12508. doi: 10.1093/nar/gkx952. PubMed
FANCI and FANCD2 have common as well as independent functions during the cellular replication stress response. Thompson EL, Yeo JE, Lee EA, Kan Y, Raghunandan M, Wiek C, Hanenberg H, Scharer OD, Hendrickson EA, Sobeck A. Nucleic Acids Res. 2017 Nov 16;45(20):11837-11857. doi: 10.1093/nar/gkx847. PubMed
From gRNA Identification to the Restoration of Dystrophin Expression: A Dystrophin Gene Correction Strategy for Duchenne Muscular Dystrophy Mutations Using the CRISPR-Induced Deletion Method. Duchene B, Iyombe-Engembe JP, Rousseau J, Tremblay JP, Ouellet DL. Methods Mol Biol. 2018;1687:267-283. doi: 10.1007/978-1-4939-7374-3_19. PubMed
Genome Editing of Erythroid Cell Culture Model Systems. Yik JJ, Crossley M, Quinlan KGR. Methods Mol Biol. 2018;1698:245-257. doi: 10.1007/978-1-4939-7428-3_15. PubMed
Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells. Schimmel J, Kool H, van Schendel R, Tijsterman M. EMBO J. 2017 Dec 15;36(24):3634-3649. doi: 10.15252/embj.201796948. Epub 2017 Oct 27. PubMed
miRNA independent hepacivirus variants suggest a strong evolutionary pressure to maintain miR-122 dependence. Yu Y, Scheel TKH, Luna JM, Chung H, Nishiuchi E, Scull MA, Echeverria N, Ricardo-Lax I, Kapoor A, Lipkin WI, Divers TJ, Antczak DF, Tennant BC, Rice CM. PLoS Pathog. 2017 Oct 30;13(10):e1006694. doi: 10.1371/journal.ppat.1006694. eCollection 2017 Oct. PubMed
IRAK4 is essential for TLR9-induced suppression of Epstein-Barr virus BZLF1 transcription in Akata Burkitt's lymphoma cells. Jordi M, Marty J, Mordasini V, Lunemann A, McComb S, Bernasconi M, Nadal D. PLoS One. 2017 Oct 31;12(10):e0186614. doi: 10.1371/journal.pone.0186614. eCollection 2017. PubMed
Widespread activation of antisense transcription of the host genome during herpes simplex virus 1 infection. Wyler E, Menegatti J, Franke V, Kocks C, Boltengagen A, Hennig T, Theil K, Rutkowski A, Ferrai C, Baer L, Kermas L, Friedel C, Rajewsky N, Akalin A, Dolken L, Grasser F, Landthaler M. Genome Biol. 2017 Oct 31;18(1):209. doi: 10.1186/s13059-017-1329-5. PubMed
Mutations in GREB1L Cause Bilateral Kidney Agenesis in Humans and Mice. De Tomasi L, David P, Humbert C, Silbermann F, Arrondel C, Tores F, Fouquet S, Desgrange A, Niel O, Bole-Feysot C, Nitschke P, Roume J, Cordier MP, Pietrement C, Isidor B, Khau Van Kien P, Gonzales M, Saint-Frison MH, Martinovic J, Novo R, Piard J, Cabrol C, Verma IC, Puri R, Journel H, Aziza J, Gavard L, Said-Menthon MH, Heidet L, Saunier S, Jeanpierre C. Am J Hum Genet. 2017 Nov 2;101(5):803-814. doi: 10.1016/j.ajhg.2017.09.026. PubMed
Pharmacoproteomic characterisation of human colon and rectal cancer. Frejno M, Zenezini Chiozzi R, Wilhelm M, Koch H, Zheng R, Klaeger S, Ruprecht B, Meng C, Kramer K, Jarzab A, Heinzlmeir S, Johnstone E, Domingo E, Kerr D, Jesinghaus M, Slotta-Huspenina J, Weichert W, Knapp S, Feller SM, Kuster B. Mol Syst Biol. 2017 Nov 3;13(11):951. doi: 10.15252/msb.20177701. PubMed
Angiopoietin-1 is required for Schlemm's canal development in mice and humans. Thomson BR, Souma T, Tompson SW, Onay T, Kizhatil K, Siggs OM, Feng L, Whisenhunt KN, Yanovitch TL, Kalaydjieva L, Azmanov DN, Finzi S, Tanna CE, Hewitt AW, Mackey DA, Bradfield YS, Souzeau E, Javadiyan S, Wiggs JL, Pasutto F, Liu X, John SW, Craig JE, Jin J, Young TL, Quaggin SE. J Clin Invest. 2017 Dec 1;127(12):4421-4436. doi: 10.1172/JCI95545. Epub 2017 Nov 6. PubMed
Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. Tamura N, Nishimura T, Sakamaki Y, Koyama-Honda I, Yamamoto H, Mizushima N. FEBS Lett. 2017 Dec;591(23):3819-3830. doi: 10.1002/1873-3468.12901. Epub 2017 Nov 20. PubMed

Associated Plasmids

Monitoring of Dual CRISPR/Cas9-Mediated Steroidogenic Acute Regulatory Protein Gene Deletion and Cholesterol Accumulation Using High-Resolution Fluorescence In Situ Hybridization in a Single Cell. Lee J, Jefcoate C. Front Endocrinol (Lausanne). 2017 Oct 25;8:289. doi: 10.3389/fendo.2017.00289. eCollection 2017. PubMed
A Transgenic Core Facility's Experience in Genome Editing Revolution. Yuan CL, Hu YC. Adv Exp Med Biol. 2017;1016:75-90. doi: 10.1007/978-3-319-63904-8_4. PubMed
Development of a genetic sensor that eliminates p53 deficient cells. Mircetic J, Dietrich A, Paszkowski-Rogacz M, Krause M, Buchholz F. Nat Commun. 2017 Nov 13;8(1):1463. doi: 10.1038/s41467-017-01688-w. PubMed
Fluorescently-tagged human eIF3 for single-molecule spectroscopy. Johnson AG, Petrov AN, Fuchs G, Majzoub K, Grosely R, Choi J, Puglisi JD. Nucleic Acids Res. 2018 Jan 25;46(2):e8. doi: 10.1093/nar/gkx1050. PubMed
Disrupting glucose-6-phosphate isomerase fully suppresses the "Warburg effect" and activates OXPHOS with minimal impact on tumor growth except in hypoxia. de Padua MC, Delodi G, Vucetic M, Durivault J, Vial V, Bayer P, Noleto GR, Mazure NM, Zdralevic M, Pouyssegur J. Oncotarget. 2017 Sep 18;8(50):87623-87637. doi: 10.18632/oncotarget.21007. eCollection 2017 Oct 20. PubMed
Galphai is required for carvedilol-induced beta1 adrenergic receptor beta-arrestin biased signaling. Wang J, Hanada K, Staus DP, Makara MA, Dahal GR, Chen Q, Ahles A, Engelhardt S, Rockman HA. Nat Commun. 2017 Nov 22;8(1):1706. doi: 10.1038/s41467-017-01855-z. PubMed
Novel inactivating mutations of the DCAF17 gene in American and Turkish families cause male infertility and female subfertility in the mouse model. Gurbuz F, Desai S, Diao F, Turkkahraman D, Wranitz F, Wood-Trageser M, Shin YH, Kotan LD, Jiang H, Witchel S, Gurtunca N, Yatsenko S, Mysliwec D, Topaloglu K, Rajkovic A. Clin Genet. 2018 Apr;93(4):853-859. doi: 10.1111/cge.13183. Epub 2018 Jan 24. PubMed
GSDME mediates caspase-3-dependent pyroptosis in gastric cancer. Wang Y, Yin B, Li D, Wang G, Han X, Sun X. Biochem Biophys Res Commun. 2018 Jan 1;495(1):1418-1425. doi: 10.1016/j.bbrc.2017.11.156. Epub 2017 Nov 26. PubMed
Repair of the TGFBI gene in human corneal keratocytes derived from a granular corneal dystrophy patient via CRISPR/Cas9-induced homology-directed repair. Taketani Y, Kitamoto K, Sakisaka T, Kimakura M, Toyono T, Yamagami S, Amano S, Kuroda M, Moore T, Usui T, Ouchi Y. Sci Rep. 2017 Dec 1;7(1):16713. doi: 10.1038/s41598-017-16308-2. PubMed
Polycomb subunit BMI1 determines uterine progesterone responsiveness essential for normal embryo implantation. Xin Q, Kong S, Yan J, Qiu J, He B, Zhou C, Ni Z, Bao H, Huang L, Lu J, Xia G, Liu X, Chen ZJ, Wang C, Wang H. J Clin Invest. 2018 Jan 2;128(1):175-189. doi: 10.1172/JCI92862. Epub 2017 Nov 20. PubMed
Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. Adikusuma F, Pfitzner C, Thomas PQ. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. PubMed

Associated Plasmids

Novel Mutation in FLNC (Filamin C) Causes Familial Restrictive Cardiomyopathy. Tucker NR, McLellan MA, Hu D, Ye J, Parsons VA, Mills RW, Clauss S, Dolmatova E, Shea MA, Milan DJ, Scott NS, Lindsay M, Lubitz SA, Domian IJ, Stone JR, Lin H, Ellinor PT. Circ Cardiovasc Genet. 2017 Dec;10(6). pii: CIRCGENETICS.117.001780. doi: 10.1161/CIRCGENETICS.117.001780. PubMed
A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity. Sachs N, de Ligt J, Kopper O, Gogola E, Bounova G, Weeber F, Balgobind AV, Wind K, Gracanin A, Begthel H, Korving J, van Boxtel R, Duarte AA, Lelieveld D, van Hoeck A, Ernst RF, Blokzijl F, Nijman IJ, Hoogstraat M, van de Ven M, Egan DA, Zinzalla V, Moll J, Boj SF, Voest EE, Wessels L, van Diest PJ, Rottenberg S, Vries RGJ, Cuppen E, Clevers H. Cell. 2018 Jan 11;172(1-2):373-386.e10. doi: 10.1016/j.cell.2017.11.010. Epub 2017 Dec 7. PubMed
Mechanoactivation of the angiotensin II type 1 receptor induces beta-arrestin-biased signaling through Galphai coupling. Wang J, Hanada K, Gareri C, Rockman HA. J Cell Biochem. 2018 Apr;119(4):3586-3597. doi: 10.1002/jcb.26552. Epub 2018 Jan 4. PubMed
Ubiquitination Regulates the Proteasomal Degradation and Nuclear Translocation of the Fat Mass and Obesity-Associated (FTO) Protein. Zhu T, Yong XLH, Xia D, Widagdo J, Anggono V. J Mol Biol. 2018 Feb 2;430(3):363-371. doi: 10.1016/j.jmb.2017.12.003. Epub 2017 Dec 10. PubMed
Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Hosono Y, Niknafs YS, Prensner JR, Iyer MK, Dhanasekaran SM, Mehra R, Pitchiaya S, Tien J, Escara-Wilke J, Poliakov A, Chu SC, Saleh S, Sankar K, Su F, Guo S, Qiao Y, Freier SM, Bui HH, Cao X, Malik R, Johnson TM, Beer DG, Feng FY, Zhou W, Chinnaiyan AM. Cell. 2017 Dec 14;171(7):1559-1572.e20. doi: 10.1016/j.cell.2017.11.040. PubMed
Digenic mutations on SCAP and AGXT2 predispose to premature myocardial infarction. Gao Y, Lee C, Song J, Li S, Cui Y, Liu Y, Wang J, Lu F, Chen H. Oncotarget. 2017 Oct 24;8(59):100141-100149. doi: 10.18632/oncotarget.22045. eCollection 2017 Nov 21. PubMed
Replication Stress Shapes a Protective Chromatin Environment across Fragile Genomic Regions. Kim J, Sturgill D, Sebastian R, Khurana S, Tran AD, Edwards GB, Kruswick A, Burkett S, Hosogane EK, Hannon WW, Weyemi U, Bonner WM, Luger K, Oberdoerffer P. Mol Cell. 2018 Jan 4;69(1):36-47.e7. doi: 10.1016/j.molcel.2017.11.021. Epub 2017 Dec 14. PubMed
Distinct SoxB1 networks are required for naive and primed pluripotency. Corsinotti A, Wong FC, Tatar T, Szczerbinska I, Halbritter F, Colby D, Gogolok S, Pantier R, Liggat K, Mirfazeli ES, Hall-Ponsele E, Mullin NP, Wilson V, Chambers I. Elife. 2017 Dec 19;6. pii: 27746. doi: 10.7554/eLife.27746. PubMed
Apoptosis induction and inhibition of HeLa cell proliferation by alpha-naphthoflavone and resveratrol are aryl hydrocarbon receptor-independent. Flores-Perez A, Elizondo G. Chem Biol Interact. 2018 Feb 1;281:98-105. doi: 10.1016/j.cbi.2017.12.029. Epub 2017 Dec 20. PubMed
CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog. Pusapati GV, Kong JH, Patel BB, Krishnan A, Sagner A, Kinnebrew M, Briscoe J, Aravind L, Rohatgi R. Dev Cell. 2018 Jan 8;44(1):113-129.e8. doi: 10.1016/j.devcel.2017.12.003. Epub 2017 Dec 28. PubMed
The deubiquitinating enzyme cylindromatosis mitigates nonalcoholic steatohepatitis. Ji YX, Huang Z, Yang X, Wang X, Zhao LP, Wang PX, Zhang XJ, Alves-Bezerra M, Cai L, Zhang P, Lu YX, Bai L, Gao MM, Zhao H, Tian S, Wang Y, Huang ZX, Zhu XY, Zhang Y, Gong J, She ZG, Li F, Cohen DE, Li H. Nat Med. 2018 Feb;24(2):213-223. doi: 10.1038/nm.4461. Epub 2018 Jan 1. PubMed
Gene editing rescue of a novel MPL mutant associated with congenital amegakaryocytic thrombocytopenia. Cleyrat C, Girard R, Choi EH, Jeziorski E, Lavabre-Bertrand T, Hermouet S, Carillo S, Wilson BS. Blood Adv. 2017 Sep 22;1(21):1815-1826. doi: 10.1182/bloodadvances.2016002915. eCollection 2017 Sep 26. PubMed
Aldehyde dehydrogenase 1A3 (ALDH1A3) is regulated by autophagy in human glioblastoma cells. Wu W, Schecker J, Wurstle S, Schneider F, Schonfelder M, Schlegel J. Cancer Lett. 2018 Mar 28;417:112-123. doi: 10.1016/j.canlet.2017.12.036. Epub 2018 Jan 3. PubMed
Vps13D Encodes a Ubiquitin-Binding Protein that Is Required for the Regulation of Mitochondrial Size and Clearance. Anding AL, Wang C, Chang TK, Sliter DA, Powers CM, Hofmann K, Youle RJ, Baehrecke EH. Curr Biol. 2018 Jan 22;28(2):287-295.e6. doi: 10.1016/j.cub.2017.11.064. Epub 2018 Jan 4. PubMed
Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection. Steyer B, Bu Q, Cory E, Jiang K, Duong S, Sinha D, Steltzer S, Gamm D, Chang Q, Saha K. Stem Cell Reports. 2018 Feb 13;10(2):642-654. doi: 10.1016/j.stemcr.2017.12.004. Epub 2018 Jan 4. PubMed
The WD40 domain of ATG16L1 is required for its non-canonical role in lipidation of LC3 at single membranes. Fletcher K, Ulferts R, Jacquin E, Veith T, Gammoh N, Arasteh JM, Mayer U, Carding SR, Wileman T, Beale R, Florey O. EMBO J. 2018 Feb 15;37(4). pii: embj.201797840. doi: 10.15252/embj.201797840. Epub 2018 Jan 9. PubMed
The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via alpha-TAT1 gene expression. Fernandez-Barrera J, Bernabe-Rubio M, Casares-Arias J, Rangel L, Fernandez-Martin L, Correas I, Alonso MA. J Cell Biol. 2018 Mar 5;217(3):929-944. doi: 10.1083/jcb.201702157. Epub 2018 Jan 10. PubMed
The glutamine transporter ASCT2 (SLC1A5) promotes tumor growth independently of the amino acid transporter LAT1 (SLC7A5). Cormerais Y, Massard PA, Vucetic M, Giuliano S, Tambutte E, Durivault J, Vial V, Endou H, Wempe MF, Parks SK, Pouyssegur J. J Biol Chem. 2018 Feb 23;293(8):2877-2887. doi: 10.1074/jbc.RA117.001342. Epub 2018 Jan 11. PubMed
Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11. Lagrange B, Benaoudia S, Wallet P, Magnotti F, Provost A, Michal F, Martin A, Di Lorenzo F, Py BF, Molinaro A, Henry T. Nat Commun. 2018 Jan 16;9(1):242. doi: 10.1038/s41467-017-02682-y. PubMed
Constitutive centromere-associated network contacts confer differential stability on CENP-A nucleosomes in vitro and in the cell. Cao S, Zhou K, Zhang Z, Luger K, Straight AF. Mol Biol Cell. 2018 Mar 15;29(6):751-762. doi: 10.1091/mbc.E17-10-0596. Epub 2018 Jan 17. PubMed
Lack of beta-arrestin signaling in the absence of active G proteins. Grundmann M, Merten N, Malfacini D, Inoue A, Preis P, Simon K, Ruttiger N, Ziegler N, Benkel T, Schmitt NK, Ishida S, Muller I, Reher R, Kawakami K, Inoue A, Rick U, Kuhl T, Imhof D, Aoki J, Konig GM, Hoffmann C, Gomeza J, Wess J, Kostenis E. Nat Commun. 2018 Jan 23;9(1):341. doi: 10.1038/s41467-017-02661-3. PubMed
Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens. de Groot R, Luthi J, Lindsay H, Holtackers R, Pelkmans L. Mol Syst Biol. 2018 Jan 23;14(1):e8064. doi: 10.15252/msb.20178064. PubMed
Tissue-selective effects of nucleolar stress and rDNA damage in developmental disorders. Calo E, Gu B, Bowen ME, Aryan F, Zalc A, Liang J, Flynn RA, Swigut T, Chang HY, Attardi LD, Wysocka J. Nature. 2018 Feb 1;554(7690):112-117. doi: 10.1038/nature25449. Epub 2018 Jan 24. PubMed
Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC, Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW. Cell. 2018 Jan 25;172(3):590-604.e13. doi: 10.1016/j.cell.2017.12.032. PubMed

Associated Plasmids

Molecular engineering of antibodies for site-specific covalent conjugation using CRISPR/Cas9. Khoshnejad M, Brenner JS, Motley W, Parhiz H, Greineder CF, Villa CH, Marcos-Contreras OA, Tsourkas A, Muzykantov VR. Sci Rep. 2018 Jan 29;8(1):1760. doi: 10.1038/s41598-018-19784-2. PubMed
Local control of intracellular microtubule dynamics by EB1 photodissociation. van Haren J, Charafeddine RA, Ettinger A, Wang H, Hahn KM, Wittmann T. Nat Cell Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 10.1038/s41556-017-0028-5. PubMed
A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing. Jia S, Zhang R, Lin G, Peng R, Gao P, Han Y, Fu Y, Ding J, Wu Q, Zhang K, Xie J, Li J. J Clin Lab Anal. 2018 Jun;32(5):e22391. doi: 10.1002/jcla.22391. Epub 2018 Jan 30. PubMed
Generation of PTEN knockout bone marrow mesenchymal stem cell lines by CRISPR/Cas9-mediated genome editing. Shen Y, Zhang J, Yu T, Qi C. Cytotechnology. 2018 Apr;70(2):783-791. doi: 10.1007/s10616-017-0183-3. Epub 2018 Jan 31. PubMed
Correction of diverse muscular dystrophy mutations in human engineered heart muscle by single-site genome editing. Long C, Li H, Tiburcy M, Rodriguez-Caycedo C, Kyrychenko V, Zhou H, Zhang Y, Min YL, Shelton JM, Mammen PPA, Liaw NY, Zimmermann WH, Bassel-Duby R, Schneider JW, Olson EN. Sci Adv. 2018 Jan 31;4(1):eaap9004. doi: 10.1126/sciadv.aap9004. eCollection 2018 Jan. PubMed
Translational control of ERK signaling through miRNA/4EHP-directed silencing. Jafarnejad SM, Chapat C, Matta-Camacho E, Gelbart IA, Hesketh GG, Arguello M, Garzia A, Kim SH, Attig J, Shapiro M, Morita M, Khoutorsky A, Alain T, Gkogkas CG, Stern-Ginossar N, Tuschl T, Gingras AC, Duchaine TF, Sonenberg N. Elife. 2018 Feb 7;7. pii: 35034. doi: 10.7554/eLife.35034. PubMed
miR-195 targets cyclin D3 and survivin to modulate the tumorigenesis of non-small cell lung cancer. Yu X, Zhang Y, Cavazos D, Ma X, Zhao Z, Du L, Pertsemlidis A. Cell Death Dis. 2018 Feb 7;9(2):193. doi: 10.1038/s41419-017-0219-9. PubMed
Reactivating TP53 signaling by the novel MDM2 inhibitor DS-3032b as a therapeutic option for high-risk neuroblastoma. Arnhold V, Schmelz K, Proba J, Winkler A, Wunschel J, Toedling J, Deubzer HE, Kunkele A, Eggert A, Schulte JH, Hundsdoerfer P. Oncotarget. 2017 Dec 18;9(2):2304-2319. doi: 10.18632/oncotarget.23409. eCollection 2018 Jan 5. PubMed
The IAP family member BRUCE regulates autophagosome-lysosome fusion. Ebner P, Poetsch I, Deszcz L, Hoffmann T, Zuber J, Ikeda F. Nat Commun. 2018 Feb 9;9(1):599. doi: 10.1038/s41467-018-02823-x. PubMed
RNA-Seq Analysis of IL-1B and IL-36 Responses in Epidermal Keratinocytes Identifies a Shared MyD88-Dependent Gene Signature. Swindell WR, Beamer MA, Sarkar MK, Loftus S, Fullmer J, Xing X, Ward NL, Tsoi LC, Kahlenberg MJ, Liang Y, Gudjonsson JE. Front Immunol. 2018 Jan 29;9:80. doi: 10.3389/fimmu.2018.00080. eCollection 2018. PubMed
Overlapping Role of SCYL1 and SCYL3 in Maintaining Motor Neuron Viability. Kuliyev E, Gingras S, Guy CS, Howell S, Vogel P, Pelletier S. J Neurosci. 2018 Feb 7. pii: JNEUROSCI.2282-17.2018. doi: 10.1523/JNEUROSCI.2282-17.2018. PubMed
Pgam5 released from damaged mitochondria induces mitochondrial biogenesis via Wnt signaling. Bernkopf DB, Jalal K, Bruckner M, Knaup KX, Gentzel M, Schambony A, Behrens J. J Cell Biol. 2018 Apr 2;217(4):1383-1394. doi: 10.1083/jcb.201708191. Epub 2018 Feb 8. PubMed
Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B-cell programming. Li R, Cauchy P, Ramamoorthy S, Boller S, Chavez L, Grosschedl R. Genes Dev. 2018 Jan 15;32(2):96-111. doi: 10.1101/gad.309583.117. Epub 2018 Feb 9. PubMed
Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells. Kornete M, Marone R, Jeker LT. J Immunol. 2018 Feb 14. pii: jimmunol.1701121. doi: 10.4049/jimmunol.1701121. PubMed

Associated Plasmids

Actin retrograde flow controls natural killer cell response by regulating the conformation state of SHP-1. Matalon O, Ben-Shmuel A, Kivelevitz J, Sabag B, Fried S, Joseph N, Noy E, Biber G, Barda-Saad M. EMBO J. 2018 Mar 1;37(5). pii: embj.201696264. doi: 10.15252/embj.201696264. Epub 2018 Feb 15. PubMed
The exoribonuclease Xrn1 is a post-transcriptional negative regulator of autophagy. Delorme-Axford E, Abernathy E, Lennemann NJ, Bernard A, Ariosa A, Coyne CB, Kirkegaard K, Klionsky DJ. Autophagy. 2018;14(5):898-912. doi: 10.1080/15548627.2018.1441648. Epub 2018 Mar 21. PubMed
Intragenic origins due to short G1 phases underlie oncogene-induced DNA replication stress. Macheret M, Halazonetis TD. Nature. 2018 Mar 1;555(7694):112-116. doi: 10.1038/nature25507. Epub 2018 Feb 21. PubMed
BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis. McArthur K, Whitehead LW, Heddleston JM, Li L, Padman BS, Oorschot V, Geoghegan ND, Chappaz S, Davidson S, San Chin H, Lane RM, Dramicanin M, Saunders TL, Sugiana C, Lessene R, Osellame LD, Chew TL, Dewson G, Lazarou M, Ramm G, Lessene G, Ryan MT, Rogers KL, van Delft MF, Kile BT. Science. 2018 Feb 23;359(6378). pii: 359/6378/eaao6047. doi: 10.1126/science.aao6047. PubMed

Associated Plasmids

Recognition of RNA N(6)-methyladenosine by IGF2BP proteins enhances mRNA stability and translation. Huang H, Weng H, Sun W, Qin X, Shi H, Wu H, Zhao BS, Mesquita A, Liu C, Yuan CL, Hu YC, Huttelmaier S, Skibbe JR, Su R, Deng X, Dong L, Sun M, Li C, Nachtergaele S, Wang Y, Hu C, Ferchen K, Greis KD, Jiang X, Wei M, Qu L, Guan JL, He C, Yang J, Chen J. Nat Cell Biol. 2018 Mar;20(3):285-295. doi: 10.1038/s41556-018-0045-z. Epub 2018 Feb 23. PubMed
Delineation of the Exact Transcription Termination Signal for Type 3 Polymerase III. Gao Z, Herrera-Carrillo E, Berkhout B. Mol Ther Nucleic Acids. 2018 Mar 2;10:36-44. doi: 10.1016/j.omtn.2017.11.006. Epub 2017 Nov 21. PubMed
PARP14 Controls the Nuclear Accumulation of a Subset of Type I IFN-Inducible Proteins. Caprara G, Prosperini E, Piccolo V, Sigismondo G, Melacarne A, Cuomo A, Boothby M, Rescigno M, Bonaldi T, Natoli G. J Immunol. 2018 Apr 1;200(7):2439-2454. doi: 10.4049/jimmunol.1701117. Epub 2018 Mar 2. PubMed
Dissecting super-enhancer hierarchy based on chromatin interactions. Huang J, Li K, Cai W, Liu X, Zhang Y, Orkin SH, Xu J, Yuan GC. Nat Commun. 2018 Mar 5;9(1):943. doi: 10.1038/s41467-018-03279-9. PubMed
Unbiased compound-protein interface mapping and prediction of chemoresistance loci through forward genetics in haploid stem cells. Horn M, Kroef V, Allmeroth K, Schuller N, Miethe S, Peifer M, Penninger JM, Elling U, Denzel MS. Oncotarget. 2018 Jan 23;9(11):9838-9851. doi: 10.18632/oncotarget.24305. eCollection 2018 Feb 9. PubMed
Regulation of hepatic gluconeogenesis by nuclear factor Y transcription factor in mice. Zhang Y, Guan Q, Liu Y, Zhang Y, Chen Y, Chen J, Liu Y, Su Z. J Biol Chem. 2018 May 18;293(20):7894-7904. doi: 10.1074/jbc.RA117.000508. Epub 2018 Mar 12. PubMed
TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Pines A, Dijk M, Makowski M, Meulenbroek EM, Vrouwe MG, van der Weegen Y, Baltissen M, French PJ, van Royen ME, Luijsterburg MS, Mullenders LH, Vermeulen M, Vermeulen W, Pannu NS, van Attikum H. Nat Commun. 2018 Mar 12;9(1):1040. doi: 10.1038/s41467-018-03484-6. PubMed
GATA3 zinc finger 2 mutations reprogram the breast cancer transcriptional network. Takaku M, Grimm SA, Roberts JD, Chrysovergis K, Bennett BD, Myers P, Perera L, Tucker CJ, Perou CM, Wade PA. Nat Commun. 2018 Mar 13;9(1):1059. doi: 10.1038/s41467-018-03478-4. PubMed
Placentation defects are highly prevalent in embryonic lethal mouse mutants. Perez-Garcia V, Fineberg E, Wilson R, Murray A, Mazzeo CI, Tudor C, Sienerth A, White JK, Tuck E, Ryder EJ, Gleeson D, Siragher E, Wardle-Jones H, Staudt N, Wali N, Collins J, Geyer S, Busch-Nentwich EM, Galli A, Smith JC, Robertson E, Adams DJ, Weninger WJ, Mohun T, Hemberger M. Nature. 2018 Mar 22;555(7697):463-468. doi: 10.1038/nature26002. Epub 2018 Mar 14. PubMed
MUC16 overexpression induced by gene mutations promotes lung cancer cell growth and invasion. Kanwal M, Ding XJ, Song X, Zhou GB, Cao Y. Oncotarget. 2018 Jan 12;9(15):12226-12239. doi: 10.18632/oncotarget.24203. eCollection 2018 Feb 23. PubMed
4EHP-independent repression of endogenous mRNAs by the RNA-binding protein GIGYF2. Amaya Ramirez CC, Hubbe P, Mandel N, Bethune J. Nucleic Acids Res. 2018 Jun 20;46(11):5792-5808. doi: 10.1093/nar/gky198. PubMed
Site-specific O-glycosylation of members of the low-density lipoprotein receptor superfamily enhances ligand interactions. Wang S, Mao Y, Narimatsu Y, Ye Z, Tian W, Goth CK, Lira-Navarrete E, Pedersen NB, Benito-Vicente A, Martin C, Uribe KB, Hurtado-Guerrero R, Christoffersen C, Seidah NG, Nielsen R, Christensen EI, Hansen L, Bennett EP, Vakhrushev SY, Schjoldager KT, Clausen H. J Biol Chem. 2018 May 11;293(19):7408-7422. doi: 10.1074/jbc.M117.817981. Epub 2018 Mar 20. PubMed
H5N1 Influenza A Virus PB1-F2 Relieves HAX-1-Mediated Restriction of Avian Virus Polymerase PA in Human Lung Cells. Mazel-Sanchez B, Boal-Carvalho I, Silva F, Dijkman R, Schmolke M. J Virol. 2018 May 14;92(11). pii: JVI.00425-18. doi: 10.1128/JVI.00425-18. Print 2018 Jun 1. PubMed
An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression. Barbier J, Chen X, Sanchez G, Cai M, Helsmoortel M, Higuchi T, Giraud P, Contreras X, Yuan G, Feng Z, Nait-Saidi R, Deas O, Bluy L, Judde JG, Rouquier S, Ritchie W, Sakamoto S, Xie D, Kiernan R. Cell Res. 2018 May;28(5):556-571. doi: 10.1038/s41422-018-0016-8. Epub 2018 Mar 21. PubMed
Mycobacterium tuberculosis Mce2E suppresses the macrophage innate immune response and promotes epithelial cell proliferation. Qiang L, Wang J, Zhang Y, Ge P, Chai Q, Li B, Shi Y, Zhang L, Gao GF, Liu CH. Cell Mol Immunol. 2018 Mar 23. pii: 10.1038/s41423-018-0016-0. doi: 10.1038/s41423-018-0016-0. PubMed
Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling. Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J. Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483. PubMed

Associated Plasmids

PI4KIIalpha regulates insulin secretion and glucose homeostasis via a PKD-dependent pathway. Zhang L, Li J, Zhang P, Gao Z, Zhao Y, Qiao X, Chen C. Biophys Rep. 2018;4(1):25-38. doi: 10.1007/s41048-018-0049-z. Epub 2018 Mar 7. PubMed
Comparison of the editing patterns and editing efficiencies of TALEN and CRISPR-Cas9 when targeting the human CCR5 gene. Nerys-Junior A, Braga-Dias LP, Pezzuto P, Cotta-de-Almeida V, Tanuri A. Genet Mol Biol. 2018 Jan-Mar;41(1):167-179. doi: 10.1590/1678-4685-GMB-2017-0065. Epub 2018 Mar 19. PubMed
Loss of PTEN promotes formation of signaling-capable clathrin-coated pits. Rosselli-Murai LK, Yates JA, Yoshida S, Bourg J, Ho KKY, White M, Prisby J, Tan X, Altemus M, Bao L, Wu ZF, Veatch SL, Swanson JA, Merajver SD, Liu AP. J Cell Sci. 2018 Apr 26;131(8). pii: jcs.208926. doi: 10.1242/jcs.208926. PubMed
PGE2 mediates EGFR internalization and nuclear translocation via caveolin endocytosis promoting its transcriptional activity and proliferation in human NSCLC cells. Bazzani L, Donnini S, Giachetti A, Christofori G, Ziche M. Oncotarget. 2018 Feb 15;9(19):14939-14958. doi: 10.18632/oncotarget.24499. eCollection 2018 Mar 13. PubMed
Natural regulatory mutations elevate the fetal globin gene via disruption of BCL11A or ZBTB7A binding. Martyn GE, Wienert B, Yang L, Shah M, Norton LJ, Burdach J, Kurita R, Nakamura Y, Pearson RCM, Funnell APW, Quinlan KGR, Crossley M. Nat Genet. 2018 Apr;50(4):498-503. doi: 10.1038/s41588-018-0085-0. Epub 2018 Apr 2. PubMed
Long non-coding RNA THOR promotes human osteosarcoma cell growth in vitro and in vivo. Chen W, Chen M, Xu Y, Chen X, Zhou P, Zhao X, Pang F, Liang W. Biochem Biophys Res Commun. 2018 May 23;499(4):913-919. doi: 10.1016/j.bbrc.2018.04.019. Epub 2018 Apr 9. PubMed
An ADAM-10 dependent EPCR shedding links meningococcal interaction with endothelial cells to purpura fulminans. Lecuyer H, Virion Z, Barnier JP, Matczak S, Bourdoulous S, Bianchini E, Saller F, Borgel D, Nassif X, Coureuil M. PLoS Pathog. 2018 Apr 9;14(4):e1006981. doi: 10.1371/journal.ppat.1006981. eCollection 2018 Apr. PubMed
Identification of Prognostic Biomarkers by Combined mRNA and miRNA Expression Microarray Analysis in Pancreatic Cancer. Liu B, Yang H, Taher L, Denz A, Grutzmann R, Pilarsky C, Weber GF. Transl Oncol. 2018 Jun;11(3):700-714. doi: 10.1016/j.tranon.2018.03.003. Epub 2018 Apr 6. PubMed
NKX2-5 regulates human cardiomyogenesis via a HEY2 dependent transcriptional network. Anderson DJ, Kaplan DI, Bell KM, Koutsis K, Haynes JM, Mills RJ, Phelan DG, Qian EL, Leitoguinho AR, Arasaratnam D, Labonne T, Ng ES, Davis RP, Casini S, Passier R, Hudson JE, Porrello ER, Costa MW, Rafii A, Curl CL, Delbridge LM, Harvey RP, Oshlack A, Cheung MM, Mummery CL, Petrou S, Elefanty AG, Stanley EG, Elliott DA. Nat Commun. 2018 Apr 10;9(1):1373. doi: 10.1038/s41467-018-03714-x. PubMed
Stable centrosomal roots disentangle to allow interphase centriole independence. Mahen R. PLoS Biol. 2018 Apr 12;16(4):e2003998. doi: 10.1371/journal.pbio.2003998. eCollection 2018 Apr. PubMed
CRMP2-Neurofibromin Interface Drives NF1-related Pain. Moutal A, Sun L, Yang X, Li W, Cai S, Luo S, Khanna R. Neuroscience. 2018 Jun 15;381:79-90. doi: 10.1016/j.neuroscience.2018.04.002. Epub 2018 Apr 12. PubMed
miR-195 potentiates the efficacy of microtubule-targeting agents in non-small cell lung cancer. Yu X, Zhang Y, Ma X, Pertsemlidis A. Cancer Lett. 2018 Jul 28;427:85-93. doi: 10.1016/j.canlet.2018.04.007. Epub 2018 Apr 13. PubMed
STAG2 deficiency induces interferon responses via cGAS-STING pathway and restricts virus infection. Ding S, Diep J, Feng N, Ren L, Li B, Ooi YS, Wang X, Brulois KF, Yasukawa LL, Li X, Kuo CJ, Solomon DA, Carette JE, Greenberg HB. Nat Commun. 2018 Apr 16;9(1):1485. doi: 10.1038/s41467-018-03782-z. PubMed
Pluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity. Finley LWS, Vardhana SA, Carey BW, Alonso-Curbelo D, Koche R, Chen Y, Wen D, King B, Radler MR, Rafii S, Lowe SW, Allis CD, Thompson CB. Nat Cell Biol. 2018 May;20(5):565-574. doi: 10.1038/s41556-018-0086-3. Epub 2018 Apr 16. PubMed
Continuous signaling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells. He X, Klasener K, Iype JM, Becker M, Maity PC, Cavallari M, Nielsen PJ, Yang J, Reth M. EMBO J. 2018 Jun 1;37(11). pii: embj.201797980. doi: 10.15252/embj.201797980. Epub 2018 Apr 18. PubMed
IRS2 mutations linked to invasion in pleomorphic invasive lobular carcinoma. Zhu S, Ward BM, Yu J, Matthew-Onabanjo AN, Janusis J, Hsieh CC, Tomaszewicz K, Hutchinson L, Zhu LJ, Kandil D, Shaw LM. JCI Insight. 2018 Apr 19;3(8). pii: 97398. doi: 10.1172/jci.insight.97398. eCollection 2018 Apr 19. PubMed
Selection on the regulation of sympathetic nervous activity in humans and chimpanzees. Lee KS, Chatterjee P, Choi EY, Sung MK, Oh J, Won H, Park SM, Kim YJ, Yi SV, Choi JK. PLoS Genet. 2018 Apr 19;14(4):e1007311. doi: 10.1371/journal.pgen.1007311. eCollection 2018 Apr. PubMed
The atypical mitogen-activated protein kinase ERK3 is essential for establishment of epithelial architecture. Takahashi C, Miyatake K, Kusakabe M, Nishida E. J Biol Chem. 2018 Jun 1;293(22):8342-8361. doi: 10.1074/jbc.RA117.000992. Epub 2018 Apr 19. PubMed
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-Associated Endonuclease Cas9-Mediated Homology-Independent Integration for Generating Quality Control Materials for Clinical Molecular Genetic Testing. Lin G, Zhang K, Peng R, Han Y, Xie J, Li J. J Mol Diagn. 2018 May;20(3):373-380. doi: 10.1016/j.jmoldx.2018.02.002. PubMed
Enhanced Biosynthesis Performance of Heterologous Proteins in CHO-K1 Cells Using CRISPR-Cas9. Wang W, Zheng W, Hu F, He X, Wu D, Zhang W, Liu H, Ma X. ACS Synth Biol. 2018 May 18;7(5):1259-1268. doi: 10.1021/acssynbio.7b00375. Epub 2018 Apr 26. PubMed
Identification of a Novel Invasion-Promoting Region in Insulin Receptor Substrate 2. Mercado-Matos J, Janusis J, Zhu S, Chen SS, Shaw LM. Mol Cell Biol. 2018 Jun 28;38(14). pii: MCB.00590-17. doi: 10.1128/MCB.00590-17. Print 2018 Jul 15. PubMed
Smad proteins differentially regulate obesity-induced glucose and lipid abnormalities and inflammation via class-specific control of AMPK-related kinase MPK38/MELK activity. Seong HA, Manoharan R, Ha H. Cell Death Dis. 2018 May 1;9(5):471. doi: 10.1038/s41419-018-0489-x. PubMed
The GABAA Receptor beta Subunit Is Required for Inhibitory Transmission. Nguyen QA, Nicoll RA. Neuron. 2018 May 16;98(4):718-725.e3. doi: 10.1016/j.neuron.2018.03.046. Epub 2018 Apr 26. PubMed
High expression of Aurora-B is correlated with poor prognosis and drug resistance in non-small cell lung cancer. Yu J, Zhou J, Xu F, Bai W, Zhang W. Int J Biol Markers. 2018 May;33(2):215-221. doi: 10.1177/1724600817753098. Epub 2018 Apr 30. PubMed
Generation of a PAX6 knockout glioblastoma cell line with changes in cell cycle distribution and sensitivity to oxidative stress. Hegge B, Sjottem E, Mikkola I. BMC Cancer. 2018 May 2;18(1):496. doi: 10.1186/s12885-018-4394-6. PubMed
A mutation-led search for novel functional domains in MeCP2. Guy J, Alexander-Howden B, FitzPatrick L, DeSousa D, Koerner MV, Selfridge J, Bird A. Hum Mol Genet. 2018 Jul 15;27(14):2531-2545. doi: 10.1093/hmg/ddy159. PubMed
Long-term culture and cloning of primary human bronchial basal cells that maintain multipotent differentiation capacity and CFTR channel function. Peters-Hall JR, Coquelin ML, Torres MJ, LaRanger R, Alabi BR, Sho S, Calva-Moreno JF, Thomas PJ, Shay JW. Am J Physiol Lung Cell Mol Physiol. 2018 Aug 1;315(2):L313-L327. doi: 10.1152/ajplung.00355.2017. Epub 2018 May 3. PubMed
An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes. Troder SE, Ebert LK, Butt L, Assenmacher S, Schermer B, Zevnik B. PLoS One. 2018 May 3;13(5):e0196891. doi: 10.1371/journal.pone.0196891. eCollection 2018. PubMed
An integrative model for alternative polyadenylation, IntMAP, delineates mTOR-modulated endoplasmic reticulum stress response. Chang JW, Zhang W, Yeh HS, Park M, Yao C, Shi Y, Kuang R, Yong J. Nucleic Acids Res. 2018 Jul 6;46(12):5996-6008. doi: 10.1093/nar/gky340. PubMed
Inhibition of PKCdelta reduces amyloid-beta levels and reverses Alzheimer disease phenotypes. Du Y, Zhao Y, Li C, Zheng Q, Tian J, Li Z, Huang TY, Zhang W, Xu H. J Exp Med. 2018 Jun 4;215(6):1665-1677. doi: 10.1084/jem.20171193. Epub 2018 May 8. PubMed
NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence. Parry AJ, Hoare M, Bihary D, Hansel-Hertsch R, Smith S, Tomimatsu K, Mannion E, Smith A, D'Santos P, Russell IA, Balasubramanian S, Kimura H, Samarajiwa SA, Narita M. Nat Commun. 2018 May 9;9(1):1840. doi: 10.1038/s41467-018-04283-9. PubMed
Identification of a transporter complex responsible for the cytosolic entry of nitrogen-containing bisphosphonates. Yu Z, Surface LE, Park CY, Horlbeck MA, Wyant GA, Abu-Remaileh M, Peterson TR, Sabatini DM, Weissman JS, O'Shea EK. Elife. 2018 May 10;7. pii: 36620. doi: 10.7554/eLife.36620. PubMed
CD4(+) T-cell-Mediated Rejection of MHC Class II-Positive Tumor Cells Is Dependent on Antigen Secretion and Indirect Presentation on Host APCs. Haabeth OAW, Fauskanger M, Manzke M, Lundin KU, Corthay A, Bogen B, Tveita AA. Cancer Res. 2018 Aug 15;78(16):4573-4585. doi: 10.1158/0008-5472.CAN-17-2426. Epub 2018 May 11. PubMed
Importance of Validating Antibodies and Small Compound Inhibitors Using Genetic Knockout Studies-T Cell Receptor-Induced CYLD Phosphorylation by IKKepsilon/TBK1 as a Case Study. Lork M, Kreike M, Staal J, Beyaert R. Front Cell Dev Biol. 2018 Apr 10;6:40. doi: 10.3389/fcell.2018.00040. eCollection 2018. PubMed
Tumor-associated calreticulin variants functionally compromise the peptide loading complex and impair its recruitment of MHC-I. Arshad N, Cresswell P. J Biol Chem. 2018 Jun 22;293(25):9555-9569. doi: 10.1074/jbc.RA118.002836. Epub 2018 May 16. PubMed
HIV-1 Activation of Innate Immunity Depends Strongly on the Intracellular Level of TREX1 and Sensing of Incomplete Reverse Transcription Products. Kumar S, Morrison JH, Dingli D, Poeschla E. J Virol. 2018 Jul 31;92(16). pii: JVI.00001-18. doi: 10.1128/JVI.00001-18. Print 2018 Aug 15. PubMed
The orphan G-protein-coupled receptor 75 signaling is activated by the chemokine CCL5. Dedoni S, Campbell LA, Harvey BK, Avdoshina V, Mocchetti I. J Neurochem. 2018 Sep;146(5):526-539. doi: 10.1111/jnc.14463. Epub 2018 Aug 2. PubMed
Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17. Matsui T, Jiang P, Nakano S, Sakamaki Y, Yamamoto H, Mizushima N. J Cell Biol. 2018 Aug 6;217(8):2633-2645. doi: 10.1083/jcb.201712058. Epub 2018 May 22. PubMed

Associated Plasmids

Fast-Evolving Human-Specific Neural Enhancers Are Associated with Aging-Related Diseases. Chen H, Li C, Zhou Z, Liang H. Cell Syst. 2018 May 23;6(5):604-611.e4. doi: 10.1016/j.cels.2018.04.002. PubMed
The genomic landscape of TERT promoter wildtype-IDH wildtype glioblastoma. Diplas BH, He X, Brosnan-Cashman JA, Liu H, Chen LH, Wang Z, Moure CJ, Killela PJ, Loriaux DB, Lipp ES, Greer PK, Yang R, Rizzo AJ, Rodriguez FJ, Friedman AH, Friedman HS, Wang S, He Y, McLendon RE, Bigner DD, Jiao Y, Waitkus MS, Meeker AK, Yan H. Nat Commun. 2018 May 25;9(1):2087. doi: 10.1038/s41467-018-04448-6. PubMed
CRISPR-Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta-Galactoside Alpha-2,6-Sialyltransferase 1 in CHO Cells. Marx N, Grunwald-Gruber C, Bydlinski N, Dhiman H, Ngoc Nguyen L, Klanert G, Borth N. Biotechnol J. 2018 Oct;13(10):e1700217. doi: 10.1002/biot.201700217. Epub 2018 Jun 11. PubMed
Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells. Lambrus BG, Moyer TC, Holland AJ. Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. PubMed
The Tumor Suppressor CIC Directly Regulates MAPK Pathway Genes via Histone Deacetylation. Weissmann S, Cloos PA, Sidoli S, Jensen ON, Pollard S, Helin K. Cancer Res. 2018 Aug 1;78(15):4114-4125. doi: 10.1158/0008-5472.CAN-18-0342. Epub 2018 May 29. PubMed
Amino Acid Variation at VP1-145 of Enterovirus 71 Determines Attachment Receptor Usage and Neurovirulence in Human Scavenger Receptor B2 Transgenic Mice. Kobayashi K, Sudaka Y, Takashino A, Imura A, Fujii K, Koike S. J Virol. 2018 Jul 17;92(15). pii: JVI.00681-18. doi: 10.1128/JVI.00681-18. Print 2018 Aug 1. PubMed
Retrograde transport of gamma-secretase from endosomes to the trans-Golgi network regulates Abeta42 production. Kanatsu K, Hori Y, Ebinuma I, Chiu YW, Tomita T. J Neurochem. 2018 Oct;147(1):110-123. doi: 10.1111/jnc.14477. Epub 2018 Aug 16. PubMed
A CRISPR/Cas9 based engineering strategy for overexpression of multiple genes in Chinese hamster ovary cells. Eisenhut P, Klanert G, Weinguny M, Baier L, Jadhav V, Ivansson D, Borth N. Metab Eng. 2018 Jul;48:72-81. doi: 10.1016/j.ymben.2018.05.017. Epub 2018 May 28. PubMed
In vivo RNAi screening identifies Pafah1b3 as a target for combination therapy with TKIs in BCR-ABL1(+) BCP-ALL. Fiedler ERC, Bhutkar A, Lawler E, Besada R, Hemann MT. Blood Adv. 2018 Jun 12;2(11):1229-1242. doi: 10.1182/bloodadvances.2017015610. PubMed
Human-Specific NOTCH2NL Genes Affect Notch Signaling and Cortical Neurogenesis. Fiddes IT, Lodewijk GA, Mooring M, Bosworth CM, Ewing AD, Mantalas GL, Novak AM, van den Bout A, Bishara A, Rosenkrantz JL, Lorig-Roach R, Field AR, Haeussler M, Russo L, Bhaduri A, Nowakowski TJ, Pollen AA, Dougherty ML, Nuttle X, Addor MC, Zwolinski S, Katzman S, Kriegstein A, Eichler EE, Salama SR, Jacobs FMJ, Haussler D. Cell. 2018 May 31;173(6):1356-1369.e22. doi: 10.1016/j.cell.2018.03.051. Epub 2018 May 31. PubMed
CRISPR/Cas9 Mediated Disruption of the Swedish APP Allele as a Therapeutic Approach for Early-Onset Alzheimer's Disease. Gyorgy B, Loov C, Zaborowski MP, Takeda S, Kleinstiver BP, Commins C, Kastanenka K, Mu D, Volak A, Giedraitis V, Lannfelt L, Maguire CA, Joung JK, Hyman BT, Breakefield XO, Ingelsson M. Mol Ther Nucleic Acids. 2018 Jun 1;11:429-440. doi: 10.1016/j.omtn.2018.03.007. Epub 2018 Mar 16. PubMed
Acid Suspends the Circadian Clock in Hypoxia through Inhibition of mTOR. Walton ZE, Patel CH, Brooks RC, Yu Y, Ibrahim-Hashim A, Riddle M, Porcu A, Jiang T, Ecker BL, Tameire F, Koumenis C, Weeraratna AT, Welsh DK, Gillies R, Alwine JC, Zhang L, Powell JD, Dang CV. Cell. 2018 Jun 28;174(1):72-87.e32. doi: 10.1016/j.cell.2018.05.009. Epub 2018 May 31. PubMed

Associated Plasmids

APOE4 Causes Widespread Molecular and Cellular Alterations Associated with Alzheimer's Disease Phenotypes in Human iPSC-Derived Brain Cell Types. Lin YT, Seo J, Gao F, Feldman HM, Wen HL, Penney J, Cam HP, Gjoneska E, Raja WK, Cheng J, Rueda R, Kritskiy O, Abdurrob F, Peng Z, Milo B, Yu CJ, Elmsaouri S, Dey D, Ko T, Yankner BA, Tsai LH. Neuron. 2018 Jun 27;98(6):1141-1154.e7. doi: 10.1016/j.neuron.2018.05.008. Epub 2018 May 31. PubMed
A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity. Hojland Knudsen C, Asgrimsdottir ES, Rahimi K, Gill KP, Frandsen S, Hvolbol Buchholdt S, Chen M, Kjems J, Febbraro F, Denham M. Front Cell Dev Biol. 2018 May 15;6:54. doi: 10.3389/fcell.2018.00054. eCollection 2018. PubMed

Associated Plasmids

Deficient ganglioside synthesis restores responsiveness to leptin and melanocortin signaling in obese KKAy mice. Inamori KI, Ito H, Tamura Y, Nitta T, Yang X, Nihei W, Shishido F, Imazu S, Tsukita S, Yamada T, Katagiri H, Inokuchi JI. J Lipid Res. 2018 Aug;59(8):1472-1481. doi: 10.1194/jlr.M085753. Epub 2018 Jun 7. PubMed
Speed genome editing by transient CRISPR/Cas9 targeting and large DNA fragment deletion. Luo J, Lu L, Gu Y, Huang R, Gui L, Li S, Qi X, Zheng W, Chao T, Zheng Q, Liang Y, Zhang L. J Biotechnol. 2018 Jun 7;281:11-20. doi: 10.1016/j.jbiotec.2018.06.308. PubMed

Associated Plasmids

A Flat BAR Protein Promotes Actin Polymerization at the Base of Clathrin-Coated Pits. Almeida-Souza L, Frank RAW, Garcia-Nafria J, Colussi A, Gunawardana N, Johnson CM, Yu M, Howard G, Andrews B, Vallis Y, McMahon HT. Cell. 2018 Jul 12;174(2):325-337.e14. doi: 10.1016/j.cell.2018.05.020. Epub 2018 Jun 7. PubMed
Using CRISPR/Cas9 to Knock out Amylase in Acinar Cells Decreases Pancreatitis-Induced Autophagy. Yasunaga K, Ito T, Miki M, Ueda K, Fujiyama T, Tachibana Y, Fujimori N, Kawabe K, Ogawa Y. Biomed Res Int. 2018 May 17;2018:8719397. doi: 10.1155/2018/8719397. eCollection 2018. PubMed
Dual role of USP30 in controlling basal pexophagy and mitophagy. Marcassa E, Kallinos A, Jardine J, Rusilowicz-Jones EV, Martinez A, Kuehl S, Islinger M, Clague MJ, Urbe S. EMBO Rep. 2018 Jul;19(7). pii: embr.201745595. doi: 10.15252/embr.201745595. Epub 2018 Jun 12. PubMed
Alleviation of reactive oxygen species enhances PUFA accumulation in Schizochytrium sp. through regulating genes involved in lipid metabolism. Zhang S, He Y, Sen B, Chen X, Xie Y, Keasling JD, Wang G. Metab Eng Commun. 2018 Mar 27;6:39-48. doi: 10.1016/j.meteno.2018.03.002. eCollection 2018 Jun. PubMed
Disruption of a -35 kb Enhancer Impairs CTCF Binding and MLH1 Expression in Colorectal Cells. Liu Q, Thoms JAI, Nunez AC, Huang Y, Knezevic K, Packham D, Poulos RC, Williams R, Beck D, Hawkins NJ, Ward RL, Wong JWH, Hesson LB, Sloane MA, Pimanda JE. Clin Cancer Res. 2018 Sep 15;24(18):4602-4611. doi: 10.1158/1078-0432.CCR-17-3678. Epub 2018 Jun 13. PubMed
Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Zhao C, Dong C, Frah M, Deng Y, Marie C, Zhang F, Xu L, Ma Z, Dong X, Lin Y, Koenig S, Nait-Oumesmar B, Martin DM, Wu LN, Xin M, Zhou W, Parras C, Lu QR. Dev Cell. 2018 Jun 18;45(6):753-768.e8. doi: 10.1016/j.devcel.2018.05.022. PubMed
Capturing the Onset of PRC2-Mediated Repressive Domain Formation. Oksuz O, Narendra V, Lee CH, Descostes N, LeRoy G, Raviram R, Blumenberg L, Karch K, Rocha PP, Garcia BA, Skok JA, Reinberg D. Mol Cell. 2018 Jun 21;70(6):1149-1162.e5. doi: 10.1016/j.molcel.2018.05.023. PubMed
AT 101 induces early mitochondrial dysfunction and HMOX1 (heme oxygenase 1) to trigger mitophagic cell death in glioma cells. Meyer N, Zielke S, Michaelis JB, Linder B, Warnsmann V, Rakel S, Osiewacz HD, Fulda S, Mittelbronn M, Munch C, Behrends C, Kogel D. Autophagy. 2018;14(10):1693-1709. doi: 10.1080/15548627.2018.1476812. Epub 2018 Jul 21. PubMed
Novel combined Ato-C treatment synergistically suppresses proliferation of Bcr-Abl-positive leukemic cells in vitro and in vivo. Wahiduzzaman M, Ota A, Karnan S, Hanamura I, Mizuno S, Kanasugi J, Rahman ML, Hyodo T, Konishi H, Tsuzuki S, Takami A, Hosokawa Y. Cancer Lett. 2018 Oct 1;433:117-130. doi: 10.1016/j.canlet.2018.06.027. Epub 2018 Jun 23. PubMed
The Effect of GPRC5a on the Proliferation, Migration Ability, Chemotherapy Resistance, and Phosphorylation of GSK-3beta in Pancreatic Cancer. Liu B, Yang H, Pilarsky C, Weber GF. Int J Mol Sci. 2018 Jun 26;19(7). pii: ijms19071870. doi: 10.3390/ijms19071870. PubMed
Induction of innate immune memory via microRNA targeting of chromatin remodelling factors. Seeley JJ, Baker RG, Mohamed G, Bruns T, Hayden MS, Deshmukh SD, Freedberg DE, Ghosh S. Nature. 2018 Jul;559(7712):114-119. doi: 10.1038/s41586-018-0253-5. Epub 2018 Jun 27. PubMed
Evolution of Cortical Neurogenesis in Amniotes Controlled by Robo Signaling Levels. Cardenas A, Villalba A, de Juan Romero C, Pico E, Kyrousi C, Tzika AC, Tessier-Lavigne M, Ma L, Drukker M, Cappello S, Borrell V. Cell. 2018 Jul 26;174(3):590-606.e21. doi: 10.1016/j.cell.2018.06.007. Epub 2018 Jun 28. PubMed
The Cytotoxicity of Epsilon Toxin from Clostridium perfringens on Lymphocytes Is Mediated by MAL Protein Expression. Blanch M, Dorca-Arevalo J, Not A, Cases M, Gomez de Aranda I, Martinez-Yelamos A, Martinez-Yelamos S, Solsona C, Blasi J. Mol Cell Biol. 2018 Sep 14;38(19). pii: MCB.00086-18. doi: 10.1128/MCB.00086-18. Print 2018 Oct 1. PubMed
Cleavage of Toll-Like Receptor 9 Ectodomain Is Required for In Vivo Responses to Single Strand DNA. Fukui R, Yamamoto C, Matsumoto F, Onji M, Shibata T, Murakami Y, Kanno A, Hayashi T, Tanimura N, Yoshida N, Miyake K. Front Immunol. 2018 Jun 27;9:1491. doi: 10.3389/fimmu.2018.01491. eCollection 2018. PubMed
DDX3 suppresses type I interferons and favors viral replication during Arenavirus infection. Loureiro ME, Zorzetto-Fernandes AL, Radoshitzky S, Chi X, Dallari S, Marooki N, Leger P, Foscaldi S, Harjono V, Sharma S, Zid BM, Lopez N, de la Torre JC, Bavari S, Zuniga E. PLoS Pathog. 2018 Jul 12;14(7):e1007125. doi: 10.1371/journal.ppat.1007125. eCollection 2018 Jul. PubMed
DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation. Lemmens B, Hegarat N, Akopyan K, Sala-Gaston J, Bartek J, Hochegger H, Lindqvist A. Mol Cell. 2018 Jul 5;71(1):117-128.e3. doi: 10.1016/j.molcel.2018.05.026. Epub 2018 Jun 28. PubMed
In vitro-transcribed guide RNAs trigger an innate immune response via the RIG-I pathway. Wienert B, Shin J, Zelin E, Pestal K, Corn JE. PLoS Biol. 2018 Jul 16;16(7):e2005840. doi: 10.1371/journal.pbio.2005840. eCollection 2018 Jul. PubMed
The contributions of individual galactosyltransferases to protein specific N-glycan processing in Chinese Hamster Ovary cells. Bydlinski N, Maresch D, Schmieder V, Klanert G, Strasser R, Borth N. J Biotechnol. 2018 Sep 20;282:101-110. doi: 10.1016/j.jbiotec.2018.07.015. Epub 2018 Jul 11. PubMed
Photosensitivity and type I IFN responses in cutaneous lupus are driven by epidermal-derived interferon kappa. Sarkar MK, Hile GA, Tsoi LC, Xing X, Liu J, Liang Y, Berthier CC, Swindell WR, Patrick MT, Shao S, Tsou PS, Uppala R, Beamer MA, Srivastava A, Bielas SL, Harms PW, Getsios S, Elder JT, Voorhees JJ, Gudjonsson JE, Kahlenberg JM. Ann Rheum Dis. 2018 Nov;77(11):1653-1664. doi: 10.1136/annrheumdis-2018-213197. Epub 2018 Jul 18. PubMed
Mutations in microRNA processing genes in Wilms tumors derepress the IGF2 regulator PLAG1. Chen KS, Stroup EK, Budhipramono A, Rakheja D, Nichols-Vinueza D, Xu L, Stuart SH, Shukla AA, Fraire C, Mendell JT, Amatruda JF. Genes Dev. 2018 Aug 1;32(15-16):996-1007. doi: 10.1101/gad.313783.118. Epub 2018 Jul 19. PubMed
Translational Control through Differential Ribosome Pausing during Amino Acid Limitation in Mammalian Cells. Darnell AM, Subramaniam AR, O'Shea EK. Mol Cell. 2018 Jul 19;71(2):229-243.e11. doi: 10.1016/j.molcel.2018.06.041. PubMed
A Simple Bioreactor-Based Method to Generate Kidney Organoids from Pluripotent Stem Cells. Przepiorski A, Sander V, Tran T, Hollywood JA, Sorrenson B, Shih JH, Wolvetang EJ, McMahon AP, Holm TM, Davidson AJ. Stem Cell Reports. 2018 Aug 14;11(2):470-484. doi: 10.1016/j.stemcr.2018.06.018. Epub 2018 Jul 19. PubMed
CRISPR/Cas9 nickase mediated targeting of urokinase receptor gene inhibits neuroblastoma cell proliferation. Rysenkova KD, Semina EV, Karagyaur MN, Shmakova AA, Dyikanov DT, Vasiluev PA, Rubtsov YP, Rubina KA, Tkachuk VA. Oncotarget. 2018 Jun 29;9(50):29414-29430. doi: 10.18632/oncotarget.25647. eCollection 2018 Jun 29. PubMed
Single olfactory receptors set odor detection thresholds. Dewan A, Cichy A, Zhang J, Miguel K, Feinstein P, Rinberg D, Bozza T. Nat Commun. 2018 Jul 23;9(1):2887. doi: 10.1038/s41467-018-05129-0. PubMed
Local chromatin interactions contribute to expression of the fibrinogen gene cluster. Espitia Jaimes C, Fish RJ, Neerman-Arbez M. J Thromb Haemost. 2018 Oct;16(10):2070-2082. doi: 10.1111/jth.14248. Epub 2018 Aug 23. PubMed
Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments. Yoo TY, Choi JM, Conway W, Yu CH, Pappu RV, Needleman DJ. Elife. 2018 Jul 25;7. pii: 36392. doi: 10.7554/eLife.36392. PubMed

Associated Plasmids

Decoupling the impact of microRNAs on translational repression versus RNA degradation in embryonic stem cells. Freimer JW, Hu TJ, Blelloch R. Elife. 2018 Jul 25;7. pii: 38014. doi: 10.7554/eLife.38014. PubMed
Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking. Kondo Y, Fu J, Wang H, Hoover C, McDaniel JM, Steet R, Patra D, Song J, Pollard L, Cathey S, Yago T, Wiley G, Macwana S, Guthridge J, McGee S, Li S, Griffin C, Furukawa K, James JA, Ruan C, McEver RP, Wierenga KJ, Gaffney PM, Xia L. JCI Insight. 2018 Jul 26;3(14). pii: 121596. doi: 10.1172/jci.insight.121596. eCollection 2018 Jul 26. PubMed
Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins. Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S. Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. PubMed
53BP1 cooperation with the REV7-shieldin complex underpins DNA structure-specific NHEJ. Ghezraoui H, Oliveira C, Becker JR, Bilham K, Moralli D, Anzilotti C, Fischer R, Deobagkar-Lele M, Sanchiz-Calvo M, Fueyo-Marcos E, Bonham S, Kessler BM, Rottenberg S, Cornall RJ, Green CM, Chapman JR. Nature. 2018 Aug;560(7716):122-127. doi: 10.1038/s41586-018-0362-1. Epub 2018 Jul 25. PubMed
miR-122, small RNA annealing and sequence mutations alter the predicted structure of the Hepatitis C virus 5' UTR RNA to stabilize and promote viral RNA accumulation. Amador-Canizares Y, Panigrahi M, Huys A, Kunden RD, Adams HM, Schinold MJ, Wilson JA. Nucleic Acids Res. 2018 Oct 12;46(18):9776-9792. doi: 10.1093/nar/gky662. PubMed
Trafficking of cholesterol to the ER is required for NLRP3 inflammasome activation. de la Roche M, Hamilton C, Mortensen R, Jeyaprakash AA, Ghosh S, Anand PK. J Cell Biol. 2018 Oct 1;217(10):3560-3576. doi: 10.1083/jcb.201709057. Epub 2018 Jul 27. PubMed
Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation. Fuentes DR, Swigut T, Wysocka J. Elife. 2018 Aug 2;7. pii: 35989. doi: 10.7554/eLife.35989. PubMed
CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A. Leung JWC, Emery LE, Miller KM. Methods Mol Biol. 2018;1832:255-269. doi: 10.1007/978-1-4939-8663-7_14. PubMed
Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish. Yang D, Zheng X, Chen S, Wang Z, Xu W, Tan J, Hu T, Hou M, Wang W, Gu Z, Wang Q, Zhang R, Zhang Y, Liu Q. Nat Commun. 2018 Aug 3;9(1):3052. doi: 10.1038/s41467-018-04984-1. PubMed
NOVA1 regulates hTERT splicing and cell growth in non-small cell lung cancer. Ludlow AT, Wong MS, Robin JD, Batten K, Yuan L, Lai TP, Dahlson N, Zhang L, Mender I, Tedone E, Sayed ME, Wright WE, Shay JW. Nat Commun. 2018 Aug 6;9(1):3112. doi: 10.1038/s41467-018-05582-x. PubMed
CHCHD2 accumulates in distressed mitochondria and facilitates oligomerization of CHCHD10. Huang X, Wu BP, Nguyen D, Liu YT, Marani M, Hench J, Benit P, Kozjak-Pavlovic V, Rustin P, Frank S, Narendra DP. Hum Mol Genet. 2018 Nov 15;27(22):3881-3900. doi: 10.1093/hmg/ddy270. PubMed
WDR62 mediates TNFalpha-dependent JNK activation via TRAF2-MLK3 axis. Prinz E, Aviram S, Aronheim A. Mol Biol Cell. 2018 Oct 1;29(20):2470-2480. doi: 10.1091/mbc.E17-08-0504. Epub 2018 Aug 9. PubMed
Genome-wide CRISPR screen identifies TMEM41B as a gene required for autophagosome formation. Morita K, Hama Y, Izume T, Tamura N, Ueno T, Yamashita Y, Sakamaki Y, Mimura K, Morishita H, Shihoya W, Nureki O, Mano H, Mizushima N. J Cell Biol. 2018 Aug 9. pii: jcb.201804132. doi: 10.1083/jcb.201804132. PubMed

Associated Plasmids

Identification of novel GLI1 target genes and regulatory circuits in human cancer cells. Diao Y, Rahman MF, Vyatkin Y, Azatyan A, St Laurent G, Kapranov P, Zaphiropoulos PG. Mol Oncol. 2018 Oct;12(10):1718-1734. doi: 10.1002/1878-0261.12366. Epub 2018 Aug 30. PubMed
Loss of SETDB1 decompacts the inactive X chromosome in part through reactivation of an enhancer in the IL1RAPL1 gene. Sun Z, Chadwick BP. Epigenetics Chromatin. 2018 Aug 13;11(1):45. doi: 10.1186/s13072-018-0218-9. PubMed
Tumor Cell Autonomous RON Receptor Expression Promotes Prostate Cancer Growth Under Conditions of Androgen Deprivation. Brown NE, Paluch AM, Nashu MA, Komurov K, Waltz SE. Neoplasia. 2018 Sep;20(9):917-929. doi: 10.1016/j.neo.2018.07.003. Epub 2018 Aug 15. PubMed
Mutant allele quantification reveals a genetic basis for TP53 mutation-driven castration resistance in prostate cancer cells. Lei K, Sun R, Chen LH, Diplas BH, Moure CJ, Wang W, Hansen LJ, Tao Y, Chen X, Chen CJ, Greer PK, Zhao F, Yan H, Bigner DD, Huang J, He Y. Sci Rep. 2018 Aug 21;8(1):12507. doi: 10.1038/s41598-018-30062-z. PubMed
Macropinosome formation by tent pole ruffling in macrophages. Condon ND, Heddleston JM, Chew TL, Luo L, McPherson PS, Ioannou MS, Hodgson L, Stow JL, Wall AA. J Cell Biol. 2018 Nov 5;217(11):3873-3885. doi: 10.1083/jcb.201804137. Epub 2018 Aug 27. PubMed
Double genetic disruption of lactate dehydrogenases A and B is required to ablate the "Warburg effect" restricting tumor growth to oxidative metabolism. Zdralevic M, Brand A, Di Ianni L, Dettmer K, Reinders J, Singer K, Peter K, Schnell A, Bruss C, Decking SM, Koehl G, Felipe-Abrio B, Durivault J, Bayer P, Evangelista M, O'Brien T, Oefner PJ, Renner K, Pouyssegur J, Kreutz M. J Biol Chem. 2018 Oct 12;293(41):15947-15961. doi: 10.1074/jbc.RA118.004180. Epub 2018 Aug 29. PubMed
The Protein Coded by a Short Open Reading Frame, Not by the Annotated Coding Sequence, Is the Main Gene Product of the Dual-Coding Gene MIEF1. Delcourt V, Brunelle M, Roy AV, Jacques JF, Salzet M, Fournier I, Roucou X. Mol Cell Proteomics. 2018 Dec;17(12):2402-2411. doi: 10.1074/mcp.RA118.000593. Epub 2018 Sep 4. PubMed
Stage-specific epigenetic regulation of CD4 expression by coordinated enhancer elements during T cell development. Issuree PD, Day K, Au C, Raviram R, Zappile P, Skok JA, Xue HH, Myers RM, Littman DR. Nat Commun. 2018 Sep 5;9(1):3594. doi: 10.1038/s41467-018-05834-w. PubMed
Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3'-overhang. Bin Moon S, Lee JM, Kang JG, Lee NE, Ha DI, Kim DY, Kim SH, Yoo K, Kim D, Ko JH, Kim YS. Nat Commun. 2018 Sep 7;9(1):3651. doi: 10.1038/s41467-018-06129-w. PubMed
OXA1L mutations cause mitochondrial encephalopathy and a combined oxidative phosphorylation defect. Thompson K, Mai N, Olahova M, Scialo F, Formosa LE, Stroud DA, Garrett M, Lax NZ, Robertson FM, Jou C, Nascimento A, Ortez C, Jimenez-Mallebrera C, Hardy SA, He L, Brown GK, Marttinen P, McFarland R, Sanz A, Battersby BJ, Bonnen PE, Ryan MT, Chrzanowska-Lightowlers ZM, Lightowlers RN, Taylor RW. EMBO Mol Med. 2018 Nov;10(11). pii: emmm.201809060. doi: 10.15252/emmm.201809060. PubMed
Human Host Range Restriction of the Vaccinia Virus C7/K1 Double Deletion Mutant Is Mediated by an Atypical Mode of Translation Inhibition. Sivan G, Glushakow-Smith SG, Katsafanas GC, Americo JL, Moss B. J Virol. 2018 Nov 12;92(23). pii: JVI.01329-18. doi: 10.1128/JVI.01329-18. Print 2018 Dec 1. PubMed
Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs. Monfort A, Di Minin G, Wutz A. Methods Mol Biol. 2018;1861:1-18. doi: 10.1007/978-1-4939-8766-5_1. PubMed
Anthropoid primate-specific retroviral element THE1B controls expression of CRH in placenta and alters gestation length. Dunn-Fletcher CE, Muglia LM, Pavlicev M, Wolf G, Sun MA, Hu YC, Huffman E, Tumukuntala S, Thiele K, Mukherjee A, Zoubovsky S, Zhang X, Swaggart KA, Lamm KYB, Jones H, Macfarlan TS, Muglia LJ. PLoS Biol. 2018 Sep 19;16(9):e2006337. doi: 10.1371/journal.pbio.2006337. eCollection 2018 Sep. PubMed
Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels. Hayashi Y, Nemoto-Sasaki Y, Matsumoto N, Hama K, Tanikawa T, Oka S, Saeki T, Kumasaka T, Koizumi T, Arai S, Wada I, Yokoyama K, Sugiura T, Yamashita A. J Biol Chem. 2018 Nov 9;293(45):17505-17522. doi: 10.1074/jbc.RA118.002048. Epub 2018 Sep 21. PubMed
Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression. Parola C, Mason DM, Zingg A, Neumeier D, Reddy ST. Methods Mol Biol. 2018;1850:79-111. doi: 10.1007/978-1-4939-8730-6_7. PubMed
Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Fort L, Batista JM, Thomason PA, Spence HJ, Whitelaw JA, Tweedy L, Greaves J, Martin KJ, Anderson KI, Brown P, Lilla S, Neilson MP, Tafelmeyer P, Zanivan S, Ismail S, Bryant DM, Tomkinson NCO, Chamberlain LH, Mastick GS, Insall RH, Machesky LM. Nat Cell Biol. 2018 Oct;20(10):1159-1171. doi: 10.1038/s41556-018-0198-9. Epub 2018 Sep 24. PubMed
Loperamide, pimozide, and STF-62247 trigger autophagy-dependent cell death in glioblastoma cells. Zielke S, Meyer N, Mari M, Abou-El-Ardat K, Reggiori F, van Wijk SJL, Kogel D, Fulda S. Cell Death Dis. 2018 Sep 24;9(10):994. doi: 10.1038/s41419-018-1003-1. PubMed
Neuronal MAP kinase p38alpha inhibits c-Jun N-terminal kinase to modulate anxiety-related behaviour. Stefanoska K, Bertz J, Volkerling AM, van der Hoven J, Ittner LM, Ittner A. Sci Rep. 2018 Sep 24;8(1):14296. doi: 10.1038/s41598-018-32592-y. PubMed
Cationic lipid-assisted nanoparticles for delivery of mRNA cancer vaccine. Fan YN, Li M, Luo YL, Chen Q, Wang L, Zhang HB, Shen S, Gu Z, Wang J. Biomater Sci. 2018 Nov 1;6(11):3009-3018. doi: 10.1039/c8bm00908b. Epub 2018 Sep 28. PubMed
Molecular architecture and regulation of BCL10-MALT1 filaments. Schlauderer F, Seeholzer T, Desfosses A, Gehring T, Strauss M, Hopfner KP, Gutsche I, Krappmann D, Lammens K. Nat Commun. 2018 Oct 2;9(1):4041. doi: 10.1038/s41467-018-06573-8. PubMed
Somatostatin receptor subtype 5 modifies hypothalamic-pituitary-adrenal axis stress function. Yamamoto M, Ben-Shlomo A, Kameda H, Fukuoka H, Deng N, Ding Y, Melmed S. JCI Insight. 2018 Oct 4;3(19). pii: 122932. doi: 10.1172/jci.insight.122932. PubMed
CRISPR deletion of MIEN1 in breast cancer cells. Van Treuren T, Vishwanatha JK. PLoS One. 2018 Oct 4;13(10):e0204976. doi: 10.1371/journal.pone.0204976. eCollection 2018. PubMed
The Chromatin Reader ZMYND8 Regulates Igh Enhancers to Promote Immunoglobulin Class Switch Recombination. Delgado-Benito V, Rosen DB, Wang Q, Gazumyan A, Pai JA, Oliveira TY, Sundaravinayagam D, Zhang W, Andreani M, Keller L, Kieffer-Kwon KR, Pekowska A, Jung S, Driesner M, Subbotin RI, Casellas R, Chait BT, Nussenzweig MC, Di Virgilio M. Mol Cell. 2018 Nov 15;72(4):636-649.e8. doi: 10.1016/j.molcel.2018.08.042. Epub 2018 Oct 4. PubMed
SWI/SNF catalytic subunits' switch drives resistance to EZH2 inhibitors in ARID1A-mutated cells. Wu S, Fatkhutdinov N, Fukumoto T, Bitler BG, Park PH, Kossenkov AV, Trizzino M, Tang HY, Zhang L, Gardini A, Speicher DW, Zhang R. Nat Commun. 2018 Oct 8;9(1):4116. doi: 10.1038/s41467-018-06656-6. PubMed
N-acetylglucosaminyltransferase-I as a novel regulator of epithelial-mesenchymal transition. Zhang G, Isaji T, Xu Z, Lu X, Fukuda T, Gu J. FASEB J. 2019 Feb;33(2):2823-2835. doi: 10.1096/fj.201801478R. Epub 2018 Oct 11. PubMed
Probing aggrephagy using chemically-induced protein aggregates. Janssen AFJ, Katrukha EA, van Straaten W, Verlhac P, Reggiori F, Kapitein LC. Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4. PubMed

Associated Plasmids

Phosphorylation of TRIM28 Enhances the Expression of IFN-beta and Proinflammatory Cytokines During HPAIV Infection of Human Lung Epithelial Cells. Krischuns T, Gunl F, Henschel L, Binder M, Willemsen J, Schloer S, Rescher U, Gerlt V, Zimmer G, Nordhoff C, Ludwig S, Brunotte L. Front Immunol. 2018 Sep 28;9:2229. doi: 10.3389/fimmu.2018.02229. eCollection 2018. PubMed
Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5. Buxade M, Huerga Encabo H, Riera-Borrull M, Quintana-Gallardo L, Lopez-Cotarelo P, Tellechea M, Martinez-Martinez S, Redondo JM, Martin-Caballero J, Flores JM, Bosch E, Rodriguez-Fernandez JL, Aramburu J, Lopez-Rodriguez C. J Exp Med. 2018 Nov 5;215(11):2901-2918. doi: 10.1084/jem.20180314. Epub 2018 Oct 16. PubMed
A methyl-sensitive element induces bidirectional transcription in TATA-less CpG island-associated promoters. Mahpour A, Scruggs BS, Smiraglia D, Ouchi T, Gelman IH. PLoS One. 2018 Oct 17;13(10):e0205608. doi: 10.1371/journal.pone.0205608. eCollection 2018. PubMed
Long non-coding RNA ChRO1 facilitates ATRX/DAXX-dependent H3.3 deposition for transcription-associated heterochromatin reorganization. Park J, Lee H, Han N, Kwak S, Lee HT, Kim JH, Kang K, Youn BH, Yang JH, Jeong HJ, Kang JS, Kim SY, Han JW, Youn HD, Cho EJ. Nucleic Acids Res. 2018 Dec 14;46(22):11759-11775. doi: 10.1093/nar/gky923. PubMed
Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury. Ying Z, Pan C, Shao T, Liu L, Li L, Guo D, Zhang S, Yuan T, Cao R, Jiang Z, Chen S, Wang F, Wang X. Mol Cell. 2018 Nov 1;72(3):457-468.e5. doi: 10.1016/j.molcel.2018.09.011. Epub 2018 Oct 18. PubMed
Cells respond to deletion of CAV1 by increasing synthesis of extracellular matrix. Mendoza-Topaz C, Nelson G, Howard G, Hafner S, Rademacher P, Frick M, Nichols BJ. PLoS One. 2018 Oct 22;13(10):e0205306. doi: 10.1371/journal.pone.0205306. eCollection 2018. PubMed
SHOC2-MRAS-PP1 complex positively regulates RAF activity and contributes to Noonan syndrome pathogenesis. Young LC, Hartig N, Boned Del Rio I, Sari S, Ringham-Terry B, Wainwright JR, Jones GG, McCormick F, Rodriguez-Viciana P. Proc Natl Acad Sci U S A. 2018 Nov 6;115(45):E10576-E10585. doi: 10.1073/pnas.1720352115. Epub 2018 Oct 22. PubMed
Using human sequencing to guide craniofacial research. Liegel RP, Finnerty E, Blizzard L, DiStasio A, Hufnagel RB, Saal HM, Sund KL, Prows CA, Stottmann RW. Genesis. 2019 Jan;57(1):e23259. doi: 10.1002/dvg.23259. Epub 2018 Dec 21. PubMed
Quantitative evaluation of incomplete preweaning lethality in mice by using the CRISPR/Cas9 system. Nakamura T, Nakajima K, Ohnishi T, Yoshikawa T, Nakanishi M, Takumi T, Tsuboi T, Kato T. Sci Rep. 2018 Oct 30;8(1):16025. doi: 10.1038/s41598-018-34270-5. PubMed
Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Borner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D. Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9. PubMed

Associated Plasmids

Adapted ATPase domain communication overcomes the cytotoxicity of p97 inhibitors. Wei Y, Toth JI, Blanco GA, Bobkov AA, Petroski MD. J Biol Chem. 2018 Dec 28;293(52):20169-20180. doi: 10.1074/jbc.RA118.004301. Epub 2018 Oct 31. PubMed
Genetic Resistance to Avian Leukosis Viruses Induced by CRISPR/Cas9 Editing of Specific Receptor Genes in Chicken Cells. Koslova A, Kucerova D, Reinisova M, Geryk J, Trefil P, Hejnar J. Viruses. 2018 Nov 2;10(11). pii: v10110605. doi: 10.3390/v10110605. PubMed
Multiple modes of PRC2 inhibition elicit global chromatin alterations in H3K27M pediatric glioma. Stafford JM, Lee CH, Voigt P, Descostes N, Saldana-Meyer R, Yu JR, Leroy G, Oksuz O, Chapman JR, Suarez F, Modrek AS, Bayin NS, Placantonakis DG, Karajannis MA, Snuderl M, Ueberheide B, Reinberg D. Sci Adv. 2018 Oct 31;4(10):eaau5935. doi: 10.1126/sciadv.aau5935. eCollection 2018 Oct. PubMed
Establishment and characterization of CRISPR/Cas9-mediated NF2(-/-) human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma. Wahiduzzaman M, Karnan S, Ota A, Hanamura I, Murakami H, Inoko A, Rahman ML, Hyodo T, Konishi H, Tsuzuki S, Hosokawa Y. Cancer Sci. 2019 Jan;110(1):180-193. doi: 10.1111/cas.13871. Epub 2018 Dec 14. PubMed
Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells. Wu W, Chen T. Methods Mol Biol. 2019;1879:75-86. doi: 10.1007/7651_2018_115. PubMed
Enhancer deletion and allelic effects define a regulatory molecular mechanism at the VLDLR cholesterol GWAS locus. Davis JP, Vadlamudi S, Roman TS, Zeynalzadeh M, Iyengar AK, Mohlke KL. Hum Mol Genet. 2018 Nov 16. pii: 5185598. doi: 10.1093/hmg/ddy385. PubMed
Transglutaminase-mediated cross-linking of WDR54 regulates EGF receptor-signaling. Maeda A, Nishino T, Matsunaga R, Yokoyama A, Suga H, Yagi T, Konishi H. Biochim Biophys Acta Mol Cell Res. 2019 Feb;1866(2):285-295. doi: 10.1016/j.bbamcr.2018.11.009. Epub 2018 Nov 17. PubMed
Long Non-Coding RNA Modulation of VEGF-A during Hypoxia. Nieminen T, Scott TA, Lin FM, Chen Z, Yla-Herttuala S, Morris KV. Noncoding RNA. 2018 Nov 20;4(4). pii: ncrna4040034. doi: 10.3390/ncrna4040034. PubMed
BAP1 regulation of the key adaptor protein NCoR1 is critical for gamma-globin gene repression. Yu L, Jearawiriyapaisarn N, Lee MP, Hosoya T, Wu Q, Myers G, Lim KC, Kurita R, Nakamura Y, Vojtek AB, Rual JF, Engel JD. Genes Dev. 2018 Dec 1;32(23-24):1537-1549. doi: 10.1101/gad.318436.118. Epub 2018 Nov 21. PubMed
Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis. Churko JM, Garg P, Treutlein B, Venkatasubramanian M, Wu H, Lee J, Wessells QN, Chen SY, Chen WY, Chetal K, Mantalas G, Neff N, Jabart E, Sharma A, Nolan GP, Salomonis N, Wu JC. Nat Commun. 2018 Nov 21;9(1):4906. doi: 10.1038/s41467-018-07333-4. PubMed
Mannose impairs tumour growth and enhances chemotherapy. Gonzalez PS, O'Prey J, Cardaci S, Barthet VJA, Sakamaki JI, Beaumatin F, Roseweir A, Gay DM, Mackay G, Malviya G, Kania E, Ritchie S, Baudot AD, Zunino B, Mrowinska A, Nixon C, Ennis D, Hoyle A, Millan D, McNeish IA, Sansom OJ, Edwards J, Ryan KM. Nature. 2018 Nov;563(7733):719-723. doi: 10.1038/s41586-018-0729-3. Epub 2018 Nov 21. PubMed
Stearyl polyethylenimine complexed with plasmids as the core of human serum albumin nanoparticles noncovalently bound to CRISPR/Cas9 plasmids or siRNA for disrupting or silencing PD-L1 expression for immunotherapy. Cheng WJ, Chen LC, Ho HO, Lin HL, Sheu MT. Int J Nanomedicine. 2018 Nov 2;13:7079-7094. doi: 10.2147/IJN.S181440. eCollection 2018. PubMed
Deletion of an intronic HIF-2alpha binding site suppresses hypoxia-induced WT1 expression. Krueger K, Catanese L, Sciesielski LK, Kirschner KM, Scholz H. Biochim Biophys Acta Gene Regul Mech. 2019 Jan;1862(1):71-83. doi: 10.1016/j.bbagrm.2018.11.003. Epub 2018 Nov 20. PubMed
Disruption of the ZBED6 binding site in intron 3 of IGF2 by CRISPR/Cas9 leads to enhanced muscle development in Liang Guang Small Spotted pigs. Liu X, Liu H, Wang M, Li R, Zeng J, Mo D, Cong P, Liu X, Chen Y, He Z. Transgenic Res. 2019 Feb;28(1):141-150. doi: 10.1007/s11248-018-0107-9. Epub 2018 Nov 28. PubMed
Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection. Sluch VM, Chamling X, Wenger C, Duan Y, Rice DS, Zack DJ. PLoS One. 2018 Nov 29;13(11):e0201683. doi: 10.1371/journal.pone.0201683. eCollection 2018. PubMed
Functional Gene Knockout of NRF2 Increases Chemosensitivity of Human Lung Cancer A549 Cells In Vitro and in a Xenograft Mouse Model. Bialk P, Wang Y, Banas K, Kmiec EB. Mol Ther Oncolytics. 2018 Oct 18;11:75-89. doi: 10.1016/j.omto.2018.10.002. eCollection 2018 Dec 21. PubMed
FBXW7 mutations reduce binding of NOTCH1, leading to cleaved NOTCH1 accumulation and target gene activation in CLL. Close V, Close W, Kugler SJ, Reichenzeller M, Yosifov DY, Bloehdorn J, Pan L, Tausch E, Westhoff MA, Dohner H, Stilgenbauer S, Oswald F, Mertens D. Blood. 2019 Feb 21;133(8):830-839. doi: 10.1182/blood-2018-09-874529. Epub 2018 Dec 3. PubMed
Targeting of chondrocyte plasticity via connexin43 modulation attenuates cellular senescence and fosters a pro-regenerative environment in osteoarthritis. Varela-Eirin M, Varela-Vazquez A, Guitian-Caamano A, Paino CL, Mato V, Largo R, Aasen T, Tabernero A, Fonseca E, Kandouz M, Caeiro JR, Blanco A, Mayan MD. Cell Death Dis. 2018 Dec 5;9(12):1166. doi: 10.1038/s41419-018-1225-2. PubMed
In vivo cell type-specific CRISPR knockdown of dopamine beta hydroxylase reduces locus coeruleus evoked wakefulness. Yamaguchi H, Hopf FW, Li SB, de Lecea L. Nat Commun. 2018 Dec 6;9(1):5211. doi: 10.1038/s41467-018-07566-3. PubMed
A Single H1 Promoter Can Drive Both Guide RNA and Endonuclease Expression in the CRISPR-Cas9 System. Gao Z, Herrera-Carrillo E, Berkhout B. Mol Ther Nucleic Acids. 2019 Mar 1;14:32-40. doi: 10.1016/j.omtn.2018.10.016. Epub 2018 Nov 1. PubMed
The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism. Mentink RA, Rella L, Radaszkiewicz TW, Gybel T, Betist MC, Bryja V, Korswagen HC. PLoS Genet. 2018 Dec 7;14(12):e1007840. doi: 10.1371/journal.pgen.1007840. eCollection 2018 Dec. PubMed
Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor. Jiao AL, Perales R, Umbreit NT, Haswell JR, Piper ME, Adams BD, Pellman D, Kennedy S, Slack FJ. RNA. 2019 Mar;25(3):352-363. doi: 10.1261/rna.069773.118. Epub 2018 Dec 11. PubMed
Heterochromatin delays CRISPR-Cas9 mutagenesis but does not influence the outcome of mutagenic DNA repair. Kallimasioti-Pazi EM, Thelakkad Chathoth K, Taylor GC, Meynert A, Ballinger T, Kelder MJE, Lalevee S, Sanli I, Feil R, Wood AJ. PLoS Biol. 2018 Dec 12;16(12):e2005595. doi: 10.1371/journal.pbio.2005595. eCollection 2018 Dec. PubMed
ATG9A Is Overexpressed in Triple Negative Breast Cancer and Its In Vitro Extinction Leads to the Inhibition of Pro-Cancer Phenotypes. Claude-Taupin A, Fonderflick L, Gauthier T, Mansi L, Pallandre JR, Borg C, Perez V, Monnien F, Algros MP, Vigneron M, Adami P, Delage-Mourroux R, Peixoto P, Herfs M, Boyer-Guittaut M, Hervouet E. Cells. 2018 Dec 6;7(12). pii: cells7120248. doi: 10.3390/cells7120248. PubMed
Truncating PKHD1 and PKD2 mutations alter energy metabolism. Chumley P, Zhou J, Mrug S, Chacko B, Parant JM, Challa AK, Wilson LS, Berryhill TF, Barnes S, Kesterson RA, Bell PD, Darley-Usmar VM, Yoder BK, Mrug M. Am J Physiol Renal Physiol. 2019 Mar 1;316(3):F414-F425. doi: 10.1152/ajprenal.00167.2018. Epub 2018 Dec 19. PubMed
Deficiency of alpha1,6-fucosyltransferase promotes neuroinflammation by increasing the sensitivity of glial cells to inflammatory mediators. Lu X, Zhang D, Shoji H, Duan C, Zhang G, Isaji T, Wang Y, Fukuda T, Gu J. Biochim Biophys Acta Gen Subj. 2019 Mar;1863(3):598-608. doi: 10.1016/j.bbagen.2018.12.008. Epub 2018 Dec 17. PubMed
Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles. Jiu Y, Kumari R, Fenix AM, Schaible N, Liu X, Varjosalo M, Krishnan R, Burnette DT, Lappalainen P. Curr Biol. 2019 Jan 7;29(1):81-92.e5. doi: 10.1016/j.cub.2018.11.045. Epub 2018 Dec 20. PubMed
An internal ribosome entry site in the coding region of tyrosyl-DNA phosphodiesterase 2 drives alternative translation start. Chou AC, Aslanian A, Sun H, Hunter T. J Biol Chem. 2019 Feb 22;294(8):2665-2677. doi: 10.1074/jbc.RA118.006269. Epub 2018 Dec 28. PubMed
The ULK1-FBXW5-SEC23B nexus controls autophagy. Jeong YT, Simoneschi D, Keegan S, Melville D, Adler NS, Saraf A, Florens L, Washburn MP, Cavasotto CN, Fenyo D, Cuervo AM, Rossi M, Pagano M. Elife. 2018 Dec 31;7. pii: 42253. doi: 10.7554/eLife.42253. PubMed
Tubular flow activates magnesium transport in the distal convoluted tubule. Verschuren EHJ, Hoenderop JGJ, Peters DJM, Arjona FJ, Bindels RJM. FASEB J. 2019 Apr;33(4):5034-5044. doi: 10.1096/fj.201802094R. Epub 2018 Dec 31. PubMed
An atlas of genetic influences on osteoporosis in humans and mice. Morris JA, Kemp JP, Youlten SE, Laurent L, Logan JG, Chai RC, Vulpescu NA, Forgetta V, Kleinman A, Mohanty ST, Sergio CM, Quinn J, Nguyen-Yamamoto L, Luco AL, Vijay J, Simon MM, Pramatarova A, Medina-Gomez C, Trajanoska K, Ghirardello EJ, Butterfield NC, Curry KF, Leitch VD, Sparkes PC, Adoum AT, Mannan NS, Komla-Ebri DSK, Pollard AS, Dewhurst HF, Hassall TAD, Beltejar MG, Adams DJ, Vaillancourt SM, Kaptoge S, Baldock P, Cooper C, Reeve J, Ntzani EE, Evangelou E, Ohlsson C, Karasik D, Rivadeneira F, Kiel DP, Tobias JH, Gregson CL, Harvey NC, Grundberg E, Goltzman D, Adams DJ, Lelliott CJ, Hinds DA, Ackert-Bicknell CL, Hsu YH, Maurano MT, Croucher PI, Williams GR, Bassett JHD, Evans DM, Richards JB. Nat Genet. 2019 Feb;51(2):258-266. doi: 10.1038/s41588-018-0302-x. Epub 2018 Dec 31. PubMed
Knockdown of KDM2A inhibits proliferation associated with TGF-beta expression in HEK293T cell. Xu WH, Liang DY, Wang Q, Shen J, Liu QH, Peng YB. Mol Cell Biochem. 2019 Jan 2. pii: 10.1007/s11010-018-03493-5. doi: 10.1007/s11010-018-03493-5. PubMed
Differential and convergent utilization of autophagy components by positive-strand RNA viruses. Abernathy E, Mateo R, Majzoub K, van Buuren N, Bird SW, Carette JE, Kirkegaard K. PLoS Biol. 2019 Jan 4;17(1):e2006926. doi: 10.1371/journal.pbio.2006926. eCollection 2019 Jan. PubMed
GPN does not release lysosomal Ca(2+) but evokes Ca(2+) release from the ER by increasing the cytosolic pH independently of cathepsin C. Atakpa P, van Marrewijk LM, Apta-Smith M, Chakraborty S, Taylor CW. J Cell Sci. 2019 Feb 1;132(3). pii: jcs.223883. doi: 10.1242/jcs.223883. PubMed
A natural regulatory mutation in the proximal promoter elevates fetal globin expression by creating a de novo GATA1 site. Martyn GE, Wienert B, Kurita R, Nakamura Y, Quinlan KGR, Crossley M. Blood. 2019 Feb 21;133(8):852-856. doi: 10.1182/blood-2018-07-863951. Epub 2019 Jan 7. PubMed
CD44 standard isoform is involved in maintenance of cancer stem cells of a hepatocellular carcinoma cell line. Asai R, Tsuchiya H, Amisaki M, Makimoto K, Takenaga A, Sakabe T, Hoi S, Koyama S, Shiota G. Cancer Med. 2019 Feb;8(2):773-782. doi: 10.1002/cam4.1968. Epub 2019 Jan 12. PubMed
Recombinant turkey herpesvirus expressing H9 hemagglutinin providing protection against H9N2 avian influenza. Liu L, Wang T, Wang M, Tong Q, Sun Y, Pu J, Sun H, Liu J. Virology. 2019 Mar;529:7-15. doi: 10.1016/j.virol.2019.01.004. Epub 2019 Jan 4. PubMed
Translation control of the immune checkpoint in cancer and its therapeutic targeting. Xu Y, Poggio M, Jin HY, Shi Z, Forester CM, Wang Y, Stumpf CR, Xue L, Devericks E, So L, Nguyen HG, Griselin A, Gordan JD, Umetsu SE, Reich SH, Worland ST, Asthana S, Barna M, Webster KR, Cunningham JT, Ruggero D. Nat Med. 2019 Feb;25(2):301-311. doi: 10.1038/s41591-018-0321-2. Epub 2019 Jan 14. PubMed
Dystrophin Deficiency Leads to Genomic Instability in Human Pluripotent Stem Cells via NO Synthase-Induced Oxidative Stress. Jelinkova S, Fojtik P, Kohutova A, Vilotic A, Markova L, Pesl M, Jurakova T, Kruta M, Vrbsky J, Gaillyova R, Valaskova I, Frak I, Lacampagne A, Forte G, Dvorak P, Meli AC, Rotrekl V. Cells. 2019 Jan 15;8(1). pii: cells8010053. doi: 10.3390/cells8010053. PubMed
A complex between phosphatidylinositol 4-kinase IIalpha and integrin alpha3beta1 is required for N-glycan sialylation in cancer cells. Isaji T, Im S, Kameyama A, Wang Y, Fukuda T, Gu J. J Biol Chem. 2019 Mar 22;294(12):4425-4436. doi: 10.1074/jbc.RA118.005208. Epub 2019 Jan 18. PubMed
Targeting mutant TP53 as a potential therapeutic strategy for the treatment of osteosarcoma. Tang F, Min L, Seebacher NA, Li X, Zhou Y, Hornicek FJ, Wei Y, Tu C, Duan Z. J Orthop Res. 2019 Mar;37(3):789-798. doi: 10.1002/jor.24227. Epub 2019 Feb 12. PubMed
Establishment of a DeltaF508-CF promyelocytic cell line for cystic fibrosis research and drug screening. Jennings S, Ng HP, Wang G. J Cyst Fibros. 2019 Jan;18(1):44-53. doi: 10.1016/j.jcf.2018.06.007. Epub 2018 Jul 7. PubMed
In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation. Spence EF, Dube S, Uezu A, Locke M, Soderblom EJ, Soderling SH. Nat Commun. 2019 Jan 23;10(1):386. doi: 10.1038/s41467-019-08288-w. PubMed
Frequent structural variations involving programmed death ligands in Epstein-Barr virus-associated lymphomas. Kataoka K, Miyoshi H, Sakata S, Dobashi A, Couronne L, Kogure Y, Sato Y, Nishida K, Gion Y, Shiraishi Y, Tanaka H, Chiba K, Watatani Y, Kakiuchi N, Shiozawa Y, Yoshizato T, Yoshida K, Makishima H, Sanada M, Onozawa M, Teshima T, Yoshiki Y, Ishida T, Suzuki K, Shimada K, Tomita A, Kato M, Ota Y, Izutsu K, Demachi-Okamura A, Akatsuka Y, Miyano S, Yoshino T, Gaulard P, Hermine O, Takeuchi K, Ohshima K, Ogawa S. Leukemia. 2019 Jul;33(7):1687-1699. doi: 10.1038/s41375-019-0380-5. Epub 2019 Jan 25. PubMed
Overexpression of NEUROG2 and NEUROG1 in human embryonic stem cells produces a network of excitatory and inhibitory neurons. Lu C, Shi X, Allen A, Baez-Nieto D, Nikish A, Sanjana NE, Pan JQ. FASEB J. 2019 Apr;33(4):5287-5299. doi: 10.1096/fj.201801110RR. Epub 2019 Jan 30. PubMed
Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells. Zhang S, Chen T, Chen N, Gao D, Shi B, Kong S, West RC, Yuan Y, Zhi M, Wei Q, Xiang J, Mu H, Yue L, Lei X, Wang X, Zhong L, Liang H, Cao S, Belmonte JCI, Wang H, Han J. Nat Commun. 2019 Jan 30;10(1):496. doi: 10.1038/s41467-019-08378-9. PubMed
Programmed cell death ligand 1 disruption by clustered regularly interspaced short palindromic repeats/Cas9-genome editing promotes antitumor immunity and suppresses ovarian cancer progression. Yahata T, Mizoguchi M, Kimura A, Orimo T, Toujima S, Kuninaka Y, Nosaka M, Ishida Y, Sasaki I, Fukuda-Ohta Y, Hemmi H, Iwahashi N, Noguchi T, Kaisho T, Kondo T, Ino K. Cancer Sci. 2019 Apr;110(4):1279-1292. doi: 10.1111/cas.13958. Epub 2019 Feb 27. PubMed
Critical role of post-transcriptional regulation for IFN-gamma in tumor-infiltrating T cells. Salerno F, Guislain A, Freen-Van Heeren JJ, Nicolet BP, Young HA, Wolkers MC. Oncoimmunology. 2018 Oct 22;8(2):e1532762. doi: 10.1080/2162402X.2018.1532762. eCollection 2019. PubMed
Magnesium-sensitive upstream ORF controls PRL phosphatase expression to mediate energy metabolism. Hardy S, Kostantin E, Wang SJ, Hristova T, Galicia-Vazquez G, Baranov PV, Pelletier J, Tremblay ML. Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):2925-2934. doi: 10.1073/pnas.1815361116. Epub 2019 Feb 4. PubMed
Caveolin-1alpha regulates primary cilium length by controlling RhoA GTPase activity. Rangel L, Bernabe-Rubio M, Fernandez-Barrera J, Casares-Arias J, Millan J, Alonso MA, Correas I. Sci Rep. 2019 Feb 4;9(1):1116. doi: 10.1038/s41598-018-38020-5. PubMed
A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity. Cao JY, Poddar A, Magtanong L, Lumb JH, Mileur TR, Reid MA, Dovey CM, Wang J, Locasale JW, Stone E, Cole SPC, Carette JE, Dixon SJ. Cell Rep. 2019 Feb 5;26(6):1544-1556.e8. doi: 10.1016/j.celrep.2019.01.043. PubMed
Derepression of LOXL4 inhibits liver cancer growth by reactivating compromised p53. Shao J, Lu J, Zhu W, Yu H, Jing X, Wang YL, Wang X, Wang XJ. Cell Death Differ. 2019 Feb 6. pii: 10.1038/s41418-019-0293-x. doi: 10.1038/s41418-019-0293-x. PubMed
Analysis of Transcriptional Regulation in Bone Cells. Jin H, Sophocleous A, Azfer A, Ralston SH. Methods Mol Biol. 2019;1914:145-167. doi: 10.1007/978-1-4939-8997-3_8. PubMed
Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines. Dyikanov DT, Vasiluev PA, Rysenkova KD, Aleksandrushkina NA, Tyurin-Kuzmin PA, Kulebyakin KY, Rubtsov YP, Shmakova AA, Evseeva MN, Balatskiy AV, Semina EV, Rostovtseva AI, Makarevich PI, Karagyaur MN. Tissue Eng Part C Methods. 2019 Mar;25(3):168-175. doi: 10.1089/ten.TEC.2018.0365. PubMed
SLFN11 can sensitize tumor cells towards IFN-gamma-mediated T cell killing. Mezzadra R, de Bruijn M, Jae LT, Gomez-Eerland R, Duursma A, Scheeren FA, Brummelkamp TR, Schumacher TN. PLoS One. 2019 Feb 12;14(2):e0212053. doi: 10.1371/journal.pone.0212053. eCollection 2019. PubMed
MARCH8 Ubiquitinates the Hepatitis C Virus Nonstructural 2 Protein and Mediates Viral Envelopment. Kumar S, Barouch-Bentov R, Xiao F, Schor S, Pu S, Biquand E, Lu A, Lindenbach BD, Jacob Y, Demeret C, Einav S. Cell Rep. 2019 Feb 12;26(7):1800-1814.e5. doi: 10.1016/j.celrep.2019.01.075. PubMed
Statins and Histone Deacetylase Inhibitors Affect Lamin A/C - Histone Deacetylase 2 Interaction in Human Cells. Mattioli E, Andrenacci D, Mai A, Valente S, Robijns J, De Vos WH, Capanni C, Lattanzi G. Front Cell Dev Biol. 2019 Jan 31;7:6. doi: 10.3389/fcell.2019.00006. eCollection 2019. PubMed
Precise tuning of gene expression levels in mammalian cells. Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, Milne TA, Cerundolo V, Fulga TA. Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y. PubMed

Associated Plasmids

Allosteric enhancement of ORP1-mediated cholesterol transport by PI(4,5)P2/PI(3,4)P2. Dong J, Du X, Wang H, Wang J, Lu C, Chen X, Zhu Z, Luo Z, Yu L, Brown AJ, Yang H, Wu JW. Nat Commun. 2019 Feb 19;10(1):829. doi: 10.1038/s41467-019-08791-0. PubMed
Targeted editing of the PSIP1 gene encoding LEDGF/p75 protects cells against HIV infection. Lampi Y, Van Looveren D, Vranckx LS, Thiry I, Bornschein S, Debyser Z, Gijsbers R. Sci Rep. 2019 Feb 20;9(1):2389. doi: 10.1038/s41598-019-38718-0. PubMed
A single-cell molecular map of mouse gastrulation and early organogenesis. Pijuan-Sala B, Griffiths JA, Guibentif C, Hiscock TW, Jawaid W, Calero-Nieto FJ, Mulas C, Ibarra-Soria X, Tyser RCV, Ho DLL, Reik W, Srinivas S, Simons BD, Nichols J, Marioni JC, Gottgens B. Nature. 2019 Feb;566(7745):490-495. doi: 10.1038/s41586-019-0933-9. Epub 2019 Feb 20. PubMed
Gene Editing of Mouse Embryonic and Epiblast Stem Cells. Sibbritt T, Osteil P, Fan X, Sun J, Salehin N, Knowles H, Shen J, Tam PPL. Methods Mol Biol. 2019;1940:77-95. doi: 10.1007/978-1-4939-9086-3_6. PubMed
The Crohn's disease associated SNP rs6651252 impacts MYC gene expression in human colonic epithelial cells. Matthews SM, Eshelman MA, Berg AS, Koltun WA, Yochum GS. PLoS One. 2019 Feb 22;14(2):e0212850. doi: 10.1371/journal.pone.0212850. eCollection 2019. PubMed
Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression in Cancer. Barger CJ, Branick C, Chee L, Karpf AR. Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. PubMed

Associated Plasmids

Precisely controlling endogenous protein dosage in hPSCs and derivatives to model FOXG1 syndrome. Zhu W, Zhang B, Li M, Mo F, Mi T, Wu Y, Teng Z, Zhou Q, Li W, Hu B. Nat Commun. 2019 Feb 25;10(1):928. doi: 10.1038/s41467-019-08841-7. PubMed
Altered splicing and cytoplasmic levels of tRNA synthetases in SF3B1-mutant myelodysplastic syndromes as a therapeutic vulnerability. Liberante FG, Lappin K, Barros EM, Vohhodina J, Grebien F, Savage KI, Mills KI. Sci Rep. 2019 Feb 25;9(1):2678. doi: 10.1038/s41598-019-39591-7. PubMed
Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis. Kizilyer A, Singh MV, Singh VB, Suwunnakorn S, Palis J, Maggirwar SB. Sci Rep. 2019 Feb 26;9(1):2781. doi: 10.1038/s41598-019-39385-x. PubMed
VEGFC acts as a double-edged sword in renal cell carcinoma aggressiveness. Ndiaye PD, Dufies M, Giuliano S, Douguet L, Grepin R, Durivault J, Lenormand P, Glisse N, Mintcheva J, Vouret-Craviari V, Mograbi B, Wurmser M, Ambrosetti D, Rioux-Leclercq N, Maire P, Pages G. Theranostics. 2019 Jan 21;9(3):661-675. doi: 10.7150/thno.27794. eCollection 2019. PubMed
Targeting the functional interplay between endoplasmic reticulum oxidoreductin-1alpha and protein disulfide isomerase suppresses the progression of cervical cancer. Zhang Y, Li T, Zhang L, Shangguan F, Shi G, Wu X, Cui Y, Wang X, Wang X, Liu Y, Lu B, Wei T, Wang CC, Wang L. EBioMedicine. 2019 Mar;41:408-419. doi: 10.1016/j.ebiom.2019.02.041. Epub 2019 Feb 27. PubMed
Chromosome segregation errors generate a diverse spectrum of simple and complex genomic rearrangements. Ly P, Brunner SF, Shoshani O, Kim DH, Lan W, Pyntikova T, Flanagan AM, Behjati S, Page DC, Campbell PJ, Cleveland DW. Nat Genet. 2019 Apr;51(4):705-715. doi: 10.1038/s41588-019-0360-8. Epub 2019 Mar 4. PubMed
Uracil-DNA glycosylase UNG1 isoform variant supports class switch recombination and repairs nuclear genomic uracil. Sarno A, Lundbaek M, Liabakk NB, Aas PA, Mjelle R, Hagen L, Sousa MML, Krokan HE, Kavli B. Nucleic Acids Res. 2019 May 21;47(9):4569-4585. doi: 10.1093/nar/gkz145. PubMed
Neuronal apolipoprotein E4 increases cell death and phosphorylated tau release in alzheimer disease. Wadhwani AR, Affaneh A, Van Gulden S, Kessler JA. Ann Neurol. 2019 May;85(5):726-739. doi: 10.1002/ana.25455. Epub 2019 Mar 27. PubMed
TAK1 suppresses RIPK1-dependent cell death and is associated with disease progression in melanoma. Podder B, Gutta C, Rozanc J, Gerlach E, Feoktistova M, Panayotova-Dimitrova D, Alexopoulos LG, Leverkus M, Rehm M. Cell Death Differ. 2019 Mar 8. pii: 10.1038/s41418-019-0315-8. doi: 10.1038/s41418-019-0315-8. PubMed
CRISPR-Cas9 corrects Duchenne muscular dystrophy exon 44 deletion mutations in mice and human cells. Min YL, Li H, Rodriguez-Caycedo C, Mireault AA, Huang J, Shelton JM, McAnally JR, Amoasii L, Mammen PPA, Bassel-Duby R, Olson EN. Sci Adv. 2019 Mar 6;5(3):eaav4324. doi: 10.1126/sciadv.aav4324. eCollection 2019 Mar. PubMed
H3K27M induces defective chromatin spread of PRC2-mediated repressive H3K27me2/me3 and is essential for glioma tumorigenesis. Harutyunyan AS, Krug B, Chen H, Papillon-Cavanagh S, Zeinieh M, De Jay N, Deshmukh S, Chen CCL, Belle J, Mikael LG, Marchione DM, Li R, Nikbakht H, Hu B, Cagnone G, Cheung WA, Mohammadnia A, Bechet D, Faury D, McConechy MK, Pathania M, Jain SU, Ellezam B, Weil AG, Montpetit A, Salomoni P, Pastinen T, Lu C, Lewis PW, Garcia BA, Kleinman CL, Jabado N, Majewski J. Nat Commun. 2019 Mar 19;10(1):1262. doi: 10.1038/s41467-019-09140-x. PubMed
[A Plasmid-Expressed CRISPR/Cas9 System Suppresses Replication of HSV Type I in a Vero Cell Culture]. Karpov DS, Karpov VL, Klimova RR, Demidova NA, Kushch AA. Mol Biol (Mosk). 2019 Jan-Feb;53(1):91-100. doi: 10.1134/S0026898419010051. PubMed
Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders. Sauter EJ, Kutsche LK, Klapper SD, Busskamp V. Methods Mol Biol. 2019;1942:101-121. doi: 10.1007/978-1-4939-9080-1_9. PubMed
Targeting intrinsic RIG-I signaling turns melanoma cells into type I interferon-releasing cellular antitumor vaccines. Bek S, Stritzke F, Wintges A, Nedelko T, Bohmer DFR, Fischer JC, Haas T, Poeck H, Heidegger S. Oncoimmunology. 2019 Feb 11;8(4):e1570779. doi: 10.1080/2162402X.2019.1570779. eCollection 2019. PubMed
Targeting FER Kinase Inhibits Melanoma Growth and Metastasis. Ivanova IA, Arulanantham S, Barr K, Cepeda M, Parkins KM, Hamilton AM, Johnston D, Penuela S, Hess DA, Ronald JA, Dagnino L. Cancers (Basel). 2019 Mar 24;11(3). pii: cancers11030419. doi: 10.3390/cancers11030419. PubMed
Collagen facilitates the colorectal cancer stemness and metastasis through an integrin/PI3K/AKT/Snail signaling pathway. Wu X, Cai J, Zuo Z, Li J. Biomed Pharmacother. 2019 Jun;114:108708. doi: 10.1016/j.biopha.2019.108708. Epub 2019 Mar 24. PubMed
Defined conditions for propagation and manipulation of mouse embryonic stem cells. Mulas C, Kalkan T, von Meyenn F, Leitch HG, Nichols J, Smith A. Development. 2019 Mar 26;146(6). pii: 146/6/dev173146. doi: 10.1242/dev.173146. PubMed
ATG9A shapes the forming autophagosome through Arfaptin 2 and phosphatidylinositol 4-kinase IIIbeta. Judith D, Jefferies HBJ, Boeing S, Frith D, Snijders AP, Tooze SA. J Cell Biol. 2019 May 6;218(5):1634-1652. doi: 10.1083/jcb.201901115. Epub 2019 Mar 27. PubMed
Human UTP14a promotes angiogenesis through upregulating PDGFA expression in colorectal cancer. Ren P, Sun X, Zhang C, Wang L, Xing B, Du X. Biochem Biophys Res Commun. 2019 May 14;512(4):871-876. doi: 10.1016/j.bbrc.2019.03.142. Epub 2019 Mar 28. PubMed
Genetic compensation triggered by mutant mRNA degradation. El-Brolosy MA, Kontarakis Z, Rossi A, Kuenne C, Gunther S, Fukuda N, Kikhi K, Boezio GLM, Takacs CM, Lai SL, Fukuda R, Gerri C, Giraldez AJ, Stainier DYR. Nature. 2019 Apr;568(7751):193-197. doi: 10.1038/s41586-019-1064-z. Epub 2019 Apr 3. PubMed
CRISPR/Cas9 genome editing of SLC37A4 gene elucidates the role of molecular markers of endoplasmic reticulum stress and apoptosis in renal involvement in glycogen storage disease type Ib. Skakic A, Andjelkovic M, Tosic N, Klaassen K, Djordjevic M, Pavlovic S, Stojiljkovic M. Gene. 2019 Jun 30;703:17-25. doi: 10.1016/j.gene.2019.04.002. Epub 2019 Apr 3. PubMed
CRISPR/Cas9 engineering of ERK5 identifies its FAK/PYK2 dependent role in adhesion-mediated cell survival. Ali M, Mutahir Z, Riaz A. Biochem Biophys Res Commun. 2019 May 21;513(1):179-185. doi: 10.1016/j.bbrc.2019.03.145. Epub 2019 Apr 2. PubMed
Generation of GLA-Knockout Human Embryonic Stem Cell Lines to Model Autophagic Dysfunction and Exosome Secretion in Fabry Disease-Associated Hypertrophic Cardiomyopathy. Song HY, Chien CS, Yarmishyn AA, Chou SJ, Yang YP, Wang ML, Wang CY, Leu HB, Yu WC, Chang YL, Chiou SH. Cells. 2019 Apr 8;8(4). pii: cells8040327. doi: 10.3390/cells8040327. PubMed
Cathelicidin is a "fire alarm", generating protective NLRP3-dependent airway epithelial cell inflammatory responses during infection with Pseudomonas aeruginosa. McHugh BJ, Wang R, Li HN, Beaumont PE, Kells R, Stevens H, Young L, Rossi AG, Gray RD, Dorin JR, Gwyer Findlay EL, Brough D, Davidson DJ. PLoS Pathog. 2019 Apr 12;15(4):e1007694. doi: 10.1371/journal.ppat.1007694. eCollection 2019 Apr. PubMed
Glutamate-Releasing SWELL1 Channel in Astrocytes Modulates Synaptic Transmission and Promotes Brain Damage in Stroke. Yang J, Vitery MDC, Chen J, Osei-Owusu J, Chu J, Qiu Z. Neuron. 2019 May 22;102(4):813-827.e6. doi: 10.1016/j.neuron.2019.03.029. Epub 2019 Apr 11. PubMed
CRISPR-Cas9-Mediated Correction of the G189R-PAX2 Mutation in Induced Pluripotent Stem Cells from a Patient with Focal Segmental Glomerulosclerosis. Trionfini P, Ciampi O, Todeschini M, Ascanelli C, Longaretti L, Perico L, Remuzzi G, Benigni A, Tomasoni S. CRISPR J. 2019 Apr;2:108-120. doi: 10.1089/crispr.2018.0048. PubMed
Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1. Harnos J, Canizal MCA, Jurasek M, Kumar J, Holler C, Schambony A, Hanakova K, Bernatik O, Zdrahal Z, Gomoryova K, Gybel T, Radaszkiewicz TW, Kravec M, Trantirek L, Rynes J, Dave Z, Fernandez-Llamazares AI, Vacha R, Tripsianes K, Hoffmann C, Bryja V. Nat Commun. 2019 Apr 18;10(1):1804. doi: 10.1038/s41467-019-09651-7. PubMed
ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer. Chen W, Schwalie PC, Pankevich EV, Gubelmann C, Raghav SK, Dainese R, Cassano M, Imbeault M, Jang SM, Russeil J, Delessa T, Duc J, Trono D, Wolfrum C, Deplancke B. Nat Commun. 2019 Apr 18;10(1):1809. doi: 10.1038/s41467-019-09803-9. PubMed
Functional evaluation of transposable elements as enhancers in mouse embryonic and trophoblast stem cells. Todd CD, Deniz O, Taylor D, Branco MR. Elife. 2019 Apr 23;8. pii: 44344. doi: 10.7554/eLife.44344. PubMed
Cyclin-Dependent Kinase 5 (CDK5)-Mediated Phosphorylation of Upstream Stimulatory Factor 2 (USF2) Contributes to Carcinogenesis. Chi TF, Horbach T, Gotz C, Kietzmann T, Dimova EY. Cancers (Basel). 2019 Apr 12;11(4). pii: cancers11040523. doi: 10.3390/cancers11040523. PubMed
Genome Editing in Mammalian Cell Lines using CRISPR-Cas. Liu KI, Sutrisnoh NB, Wang Y, Tan MH. J Vis Exp. 2019 Apr 11;(146). doi: 10.3791/59086. PubMed
Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation. Song T, Yang Y, Wei H, Xie X, Lu J, Zeng Q, Peng J, Zhou Y, Jiang S, Peng J. Nucleic Acids Res. 2019 Jul 9;47(12):6130-6144. doi: 10.1093/nar/gkz312. PubMed
Targeted deletion of BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta thalassemia disease. Khosravi MA, Abbasalipour M, Concordet JP, Berg JV, Zeinali S, Arashkia A, Azadmanesh K, Buch T, Karimipoor M. Eur J Pharmacol. 2019 Jul 5;854:398-405. doi: 10.1016/j.ejphar.2019.04.042. Epub 2019 Apr 27. PubMed
Highly efficient correction of structural mutations of 450 kb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9. Qin K, Liang X, Sun G, Shi X, Wang M, Liu H, Chen Y, Liu X, He Z. BMC Mol Cell Biol. 2019 Apr 3;20(1):4. doi: 10.1186/s12860-019-0184-5. PubMed
Ribozyme-mediated, multiplex CRISPR gene editing and CRISPR interference (CRISPRi) in rodent-infectious Plasmodium yoelii. Walker MP, Lindner SE. J Biol Chem. 2019 Jun 14;294(24):9555-9566. doi: 10.1074/jbc.RA118.007121. Epub 2019 May 1. PubMed

Associated Plasmids

Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition. Modic M, Grosch M, Rot G, Schirge S, Lepko T, Yamazaki T, Lee FCY, Rusha E, Shaposhnikov D, Palo M, Merl-Pham J, Cacchiarelli D, Rogelj B, Hauck SM, von Mering C, Meissner A, Lickert H, Hirose T, Ule J, Drukker M. Mol Cell. 2019 Jun 6;74(5):951-965.e13. doi: 10.1016/j.molcel.2019.03.041. Epub 2019 Apr 29. PubMed
An acquired scaffolding function of the DNAJ-PKAc fusion contributes to oncogenic signaling in fibrolamellar carcinoma. Turnham RE, Smith FD, Kenerson HL, Omar MH, Golkowski M, Garcia I, Bauer R, Lau HT, Sullivan KM, Langeberg LK, Ong SE, Riehle KJ, Yeung RS, Scott JD. Elife. 2019 May 7;8. pii: 44187. doi: 10.7554/eLife.44187. PubMed
Dlg1 activates beta-catenin signaling to regulate retinal angiogenesis and the blood-retina and blood-brain barriers. Cho C, Wang Y, Smallwood PM, Williams J, Nathans J. Elife. 2019 May 8;8. pii: 45542. doi: 10.7554/eLife.45542. PubMed
Effects of histone deacetylase inhibitor Scriptaid and parathyroid hormone on osteocyte functions and metabolism. Sun N, Uda Y, Azab E, Kochen A, Santos RNCE, Shi C, Kobayashi T, Wein MN, Divieti Pajevic P. J Biol Chem. 2019 Jun 21;294(25):9722-9733. doi: 10.1074/jbc.RA118.007312. Epub 2019 May 8. PubMed
Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology. Warner BK, Alder JK, Suli A. Methods Mol Biol. 2019;1965:235-250. doi: 10.1007/978-1-4939-9182-2_16. PubMed
Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency. Garcia-Tunon I, Alonso-Perez V, Vuelta E, Perez-Ramos S, Herrero M, Mendez L, Hernandez-Sanchez JM, Martin-Izquierdo M, Saldana R, Sevilla J, Sanchez-Guijo F, Hernandez-Rivas JM, Sanchez-Martin M. PLoS One. 2019 May 9;14(5):e0216674. doi: 10.1371/journal.pone.0216674. eCollection 2019. PubMed
USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. Sonego M, Pellarin I, Costa A, Vinciguerra GLR, Coan M, Kraut A, D'Andrea S, Dall'Acqua A, Castillo-Tong DC, Califano D, Losito S, Spizzo R, Coute Y, Vecchione A, Belletti B, Schiappacassi M, Baldassarre G. Sci Adv. 2019 May 8;5(5):eaav3235. doi: 10.1126/sciadv.aav3235. eCollection 2019 May. PubMed
Molecular heterogeneity in peripheral T-cell lymphoma, not otherwise specified revealed by comprehensive genetic profiling. Watatani Y, Sato Y, Miyoshi H, Sakamoto K, Nishida K, Gion Y, Nagata Y, Shiraishi Y, Chiba K, Tanaka H, Zhao L, Ochi Y, Takeuchi Y, Takeda J, Ueno H, Kogure Y, Shiozawa Y, Kakiuchi N, Yoshizato T, Nakagawa MM, Nanya Y, Yoshida K, Makishima H, Sanada M, Sakata-Yanagimoto M, Chiba S, Matsuoka R, Noguchi M, Hiramoto N, Ishikawa T, Kitagawa J, Nakamura N, Tsurumi H, Miyazaki T, Kito Y, Miyano S, Shimoda K, Takeuchi K, Ohshima K, Yoshino T, Ogawa S, Kataoka K. Leukemia. 2019 May 15. pii: 10.1038/s41375-019-0473-1. doi: 10.1038/s41375-019-0473-1. PubMed
Targeted genomic integration of EGFP under tubulin beta 3 class III promoter and mEos2 under tryptophan hydroxylase 2 promoter does not produce sufficient levels of reporter gene expression. Menzorov AG, Orishchenko KE, Fishman VS, Shevtsova AA, Mungalov RV, Pristyazhnyuk IE, Kizilova EA, Matveeva NM, Alenina N, Bader M, Rubtsov NB, Serov OL. J Cell Biochem. 2019 May 20. doi: 10.1002/jcb.28981. PubMed
Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism. Gotzl JK, Brendel M, Werner G, Parhizkar S, Sebastian Monasor L, Kleinberger G, Colombo AV, Deussing M, Wagner M, Winkelmann J, Diehl-Schmid J, Levin J, Fellerer K, Reifschneider A, Bultmann S, Bartenstein P, Rominger A, Tahirovic S, Smith ST, Madore C, Butovsky O, Capell A, Haass C. EMBO Mol Med. 2019 Jun;11(6). pii: emmm.201809711. doi: 10.15252/emmm.201809711. PubMed
ARID1A spatially partitions interphase chromosomes. Wu S, Fatkhutdinov N, Rosin L, Luppino JM, Iwasaki O, Tanizawa H, Tang HY, Kossenkov AV, Gardini A, Noma KI, Speicher DW, Joyce EF, Zhang R. Sci Adv. 2019 May 22;5(5):eaaw5294. doi: 10.1126/sciadv.aaw5294. eCollection 2019 May. PubMed
The RIG-I pathway is involved in peripheral T cell lymphopenia in patients with dermatomyositis. Zhang L, Xia Q, Li W, Peng Q, Yang H, Lu X, Wang G. Arthritis Res Ther. 2019 May 29;21(1):131. doi: 10.1186/s13075-019-1905-z. PubMed
The intraflagellar transport protein IFT20 controls lysosome biogenesis by regulating the post-Golgi transport of acid hydrolases. Finetti F, Cassioli C, Cianfanelli V, Onnis A, Paccagnini E, Kabanova A, Baldari CT. Cell Death Differ. 2019 May 29. pii: 10.1038/s41418-019-0357-y. doi: 10.1038/s41418-019-0357-y. PubMed
Targeting histone deacetylase 3 (HDAC3) in the bone marrow microenvironment inhibits multiple myeloma proliferation by modulating exosomes and IL-6 trans-signaling. Ho M, Chen T, Liu J, Dowling P, Hideshima T, Zhang L, Morelli E, Camci-Unal G, Wu X, Tai YT, Wen K, Samur M, Schlossman RL, Mazitschek R, Kavanagh EL, Lindsay S, Harada T, McCann A, Anderson KC, O'Gorman P, Bianchi G. Leukemia. 2019 May 29. pii: 10.1038/s41375-019-0493-x. doi: 10.1038/s41375-019-0493-x. PubMed
Two human patient mitochondrial pyruvate carrier mutations reveal distinct molecular mechanisms of dysfunction. Oonthonpan L, Rauckhorst AJ, Gray LR, Boutron AC, Taylor EB. JCI Insight. 2019 May 30;5. pii: 126132. doi: 10.1172/jci.insight.126132. PubMed
Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis. Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM. EMBO J. 2019 May 31. pii: embj.2018100926. doi: 10.15252/embj.2018100926. PubMed
Involvement of Interferon Regulatory Factor 7 in Nicotine's Suppression of Antiviral Immune Responses. Han H, Huang W, Du W, Shen Q, Yang Z, Li MD, Chang SL. J Neuroimmune Pharmacol. 2019 Jun 1. pii: 10.1007/s11481-019-09845-2. doi: 10.1007/s11481-019-09845-2. PubMed
Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors. Gilmour J, O'Connor L, Middleton CP, Keane P, Gillemans N, Cazier JB, Philipsen S, Bonifer C. Epigenetics Chromatin. 2019 Jun 4;12(1):33. doi: 10.1186/s13072-019-0282-9. PubMed
Isoform-specific gene disruptions reveal a role for the V-ATPase subunit a4 isoform in the invasiveness of 4T1-12B breast cancer cells. McGuire CM, Collins MP, Sun-Wada G, Wada Y, Forgac M. J Biol Chem. 2019 Jul 19;294(29):11248-11258. doi: 10.1074/jbc.RA119.007713. Epub 2019 Jun 5. PubMed
Individual and combined presenilin 1 and 2 knockouts reveal that both have highly overlapping functions in HEK293T cells. Lessard CB, Rodriguez E, Ladd TB, Minter LM, Osborne BA, Miele L, Golde TE, Ran Y. J Biol Chem. 2019 Jul 19;294(29):11276-11285. doi: 10.1074/jbc.RA119.008041. Epub 2019 Jun 5. PubMed
Histone demethylase Kdm2a regulates germ cell genes and endogenous retroviruses in embryonic stem cells. Fu E, Shen J, Dong Z, Zhang W, Zhang Y, Chen F, Cheng Z, Zhao X, Shuai L, Lu X. Epigenomics. 2019 May;11(7):751-766. doi: 10.2217/epi-2018-0126. Epub 2019 Jun 7. PubMed
SHOC2 phosphatase-dependent RAF dimerization mediates resistance to MEK inhibition in RAS-mutant cancers. Jones GG, Del Rio IB, Sari S, Sekerim A, Young LC, Hartig N, Areso Zubiaur I, El-Bahrawy MA, Hynds RE, Lei W, Molina-Arcas M, Downward J, Rodriguez-Viciana P. Nat Commun. 2019 Jun 10;10(1):2532. doi: 10.1038/s41467-019-10367-x. PubMed
Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy. Celis-Gutierrez J, Blattmann P, Zhai Y, Jarmuzynski N, Ruminski K, Gregoire C, Ounoughene Y, Fiore F, Aebersold R, Roncagalli R, Gstaiger M, Malissen B. Cell Rep. 2019 Jun 11;27(11):3315-3330.e7. doi: 10.1016/j.celrep.2019.05.041. PubMed
Rebalancing Protein Homeostasis Enhances Tumor Antigen Presentation. Jaeger AM, Stopfer L, Lee S, Gaglia G, Sandel D, Santagata S, Lin NU, Trepel JB, White F, Jacks T, Lindquist S, Whitesell L. Clin Cancer Res. 2019 Jun 18. pii: 1078-0432.CCR-19-0596. doi: 10.1158/1078-0432.CCR-19-0596. PubMed
ARID1A facilitates KRAS signaling-regulated enhancer activity in an AP1-dependent manner in colorectal cancer cells. Sen M, Wang X, Hamdan FH, Rapp J, Eggert J, Kosinsky RL, Wegwitz F, Kutschat AP, Younesi FS, Gaedcke J, Grade M, Hessmann E, Papantonis A, Strbel P, Johnsen SA. Clin Epigenetics. 2019 Jun 19;11(1):92. doi: 10.1186/s13148-019-0690-5. PubMed
A Platform for Studying Neurodegeneration Mechanisms Using Genetically Encoded Biosensors. Ustyantseva EI, Medvedev SP, Vetchinova AS, Minina JM, Illarioshkin SN, Zakian SM. Biochemistry (Mosc). 2019 Mar;84(3):299-309. doi: 10.1134/S000629791903012X. PubMed
Genetic Deletion of GABAA Receptors Reveals Distinct Requirements of Neurotransmitter Receptors for GABAergic and Glutamatergic Synapse Development. Duan J, Pandey S, Li T, Castellano D, Gu X, Li J, Tian Q, Lu W. Front Cell Neurosci. 2019 Jun 4;13:217. doi: 10.3389/fncel.2019.00217. eCollection 2019. PubMed
C/EBPbeta-LIP induces cancer-type metabolic reprogramming by regulating the let-7/LIN28B circuit in mice. Ackermann T, Hartleben G, Muller C, Mastrobuoni G, Groth M, Sterken BA, Zaini MA, Youssef SA, Zuidhof HR, Krauss SR, Kortman G, de Haan G, de Bruin A, Wang ZQ, Platzer M, Kempa S, Calkhoven CF. Commun Biol. 2019 Jun 14;2:208. doi: 10.1038/s42003-019-0461-z. eCollection 2019. PubMed
Fetal and trophoblast PI3K p110alpha have distinct roles in regulating resource supply to the growing fetus in mice. Lopez-Tello J, Perez-Garcia V, Khaira J, Kusinski LC, Cooper WN, Andreani A, Grant I, Fernandez de Liger E, Lam BY, Hemberger M, Sandovici I, Constancia M, Sferruzzi-Perri AN. Elife. 2019 Jun 26;8. pii: 45282. doi: 10.7554/eLife.45282. PubMed
Metallothionein 3 Controls the Phenotype and Metabolic Programming of Alternatively Activated Macrophages. Chowdhury D, Alrefai H, Landero Figueroa JA, Candor K, Porollo A, Fecher R, Divanovic S, Deepe GS Jr, Subramanian Vignesh K. Cell Rep. 2019 Jun 25;27(13):3873-3886.e7. doi: 10.1016/j.celrep.2019.05.093. PubMed
CRISPR/Cas9 mediated ELANE knockout enables neutrophilic maturation of primary hematopoietic stem and progenitor cells and induced pluripotent stem cells of severe congenital neutropenia patients. Nasri M, Ritter M, Mir P, Dannenmann B, Aghaallaei N, Amend D, Makaryan V, Xu Y, Fletcher B, Bernhard R, Steiert I, Hahnel K, Berger J, Koch I, Sailer B, Hipp K, Zeidler C, Klimiankou M, Bajoghli B, Dale DC, Welte K, Skokowa J. Haematologica. 2019 Jun 27. pii: haematol.2019.221804. doi: 10.3324/haematol.2019.221804. PubMed
CRISPR Base Editing in Induced Pluripotent Stem Cells. Chang YJ, Xu CL, Cui X, Bassuk AG, Mahajan VB, Tsai YT, Tsang SH. Methods Mol Biol. 2019 Jun 28. doi: 10.1007/7651_2019_243. PubMed
WDFY2 restrains matrix metalloproteinase secretion and cell invasion by controlling VAMP3-dependent recycling. Sneeggen M, Pedersen NM, Campsteijn C, Haugsten EM, Stenmark H, Schink KO. Nat Commun. 2019 Jun 28;10(1):2850. doi: 10.1038/s41467-019-10794-w. PubMed
Heterozygous loss of Srp72 in mice is not associated with major hematological phenotypes. D'Altri T, Schuster MB, Wenzel A, Porse BT. Eur J Haematol. 2019 Jun 29. doi: 10.1111/ejh.13286. PubMed
The E3 ubiquitin ligase RNF40 suppresses apoptosis in colorectal cancer cells. Schneider D, Chua RL, Molitor N, Hamdan FH, Rettenmeier EM, Prokakis E, Mishra VK, Kari V, Wegwitz F, Johnsen SA, Kosinsky RL. Clin Epigenetics. 2019 Jul 2;11(1):98. doi: 10.1186/s13148-019-0698-x. PubMed
Control of biosilica morphology and mechanical performance by the conserved diatom gene Silicanin-1. Gorlich S, Pawolski D, Zlotnikov I, Kroger N. Commun Biol. 2019 Jun 28;2:245. doi: 10.1038/s42003-019-0436-0. eCollection 2019. PubMed

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