pSP6-RNAP
(Plasmid #48143)

• Purpose: Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA
• Depositing Lab: Dieter Jahn
• Publication: Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2.

Backbone

• Vector backbone: pMGBm19
• Backbone manufacturer: Gamer et al., 2009
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Growth instructions: For Bacillus megaterium: Chloramphenicol and 37 °C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: rna polymerase SP6
• Mutation: V314A (numbering based on NCBI Sequence ID: NP_853568.1)

Resource Information

• Supplemental Documents:
  • Genbank-file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the V313A mutation in RNAP was present in the original sample and functions as described in the publication.