CBIG-Ngn2
(Plasmid #48708)

• Purpose: bicistronic expression of Ngn2 and EGFP
• Depositing Lab: Jeffrey Macklis
• Publication: Macklis lab plasmids (unpublished)

Backbone

• Vector backbone: CBIG
• Total vector size (bp): 9589
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Neurog2
• Alt name: nerogenin2
• Alt name: Ngn2
• Species: M. musculus (mouse)
• Insert Size (bp): 799
• Entrez Gene: Neurog2 (a.k.a. Atoh4, Math4A, bHLHa8, ngn-2, ngn2)
• Promoter: CMV/beta-actin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: pCDH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence from the depositing laboratory. According to the depositor, these differences are not a concern for the function of the plasmid.