8xLexA-binding elements/pMD20
(Plasmid #48807)

• Purpose: Contains 8xLexA-binding elements
• Depositing Lab: Hodaka Fujii
• Publication: Fujita et al PLoS One. 2011;6(10):e26109. doi: 10.1371/journal.pone.0026109. Epub 2011 Oct 17.

Backbone

• Vector backbone: pMD20
• Backbone manufacturer: Takara Bio
• Backbone size w/o insert (bp): 2736
• Total vector size (bp): 2951

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 8xLexA-binding elements
• Alt name: 8xLexA BE
• Species: Synthetic
• Insert Size (bp): 170

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: M13 primer M4
• 3′ sequencing primer: M13 primer RV

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Fujii Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/fujii/