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pBEG R1-ChlorR-R3 #BGV148
(Plasmid #48942)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48942 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pOK1/2
  • Backbone manufacturer
    Messing Lab, PMID 1905257
  • Backbone size (bp) 3772
  • Modifications to backbone
    The chloramphenicol selection cassette was PCR amplified from a lab Gateway destination vector (gQxiPuro, unpublished plasmid) using the following forward (5’-CACCTCTAGACTCGAGATTAGGCACCCCAGGCTTTACAC) and reverse (5’-ATATGAATTCGTCGACCTGCAGACTGGCTGTG) primers and cloned into the XbaI site of pOK1/2 B giving pOK1/2 B (ChlorR). Next, the attR1 site from pUC57 fragment A was cloned into this vector using BglII/NotI giving pBEG R1-ChlorR-R4. To create the three way destination vector (attR1-attR3) the attR4 site was replaced with attR3 from pBEG R3-L4 which was cut out with NheI/NgoMIV and cloned into the SpeI/XmaI site of pBEG R1-ChlorR-R4 creating pBEG R1-ChlorR-R3. Finally, the ccdB-ChloroR cassette from gQxiPuro was cloned into the pBEG R1-ChloroR-R3 vectors with NotI/SalI.
  • Vector type
    Gateway cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEG R1-ChlorR-R3 #BGV148 was a gift from David Dankort (Addgene plasmid # 48942 ; http://n2t.net/addgene:48942 ; RRID:Addgene_48942)
  • For your References section:

    A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D. PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852