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pYEXC3H
(Plasmid #49026)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49026 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pYES2/CT
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 7634
  • Modifications to backbone
    modified for compatibility with FX cloning and added tags/fusion proteins
  • Vector type
    Yeast Expression
  • Promoter GAL1
  • Selectable markers
    URA3
  • Tags / Fusion Proteins
    • 3C protease site (PreScission site) (C terminal on backbone)
    • decaHis (10x His) (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Low Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.

The ampicillin resistance cassette is located at bp# 3633-4493.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYEXC3H was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 49026 ; http://n2t.net/addgene:49026 ; RRID:Addgene_49026)
  • For your References section:

    A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291