pTA7002-avrPto
(Plasmid #49156)

• Purpose: Plant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPto
• Depositing Lab: Sheng Yang He
• Publication: Hauck et al Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8577-82. Epub 2003 Jun 19.

Backbone

• Vector backbone: pTA7002
• Backbone manufacturer: Nam-Hai Chua
• Vector type: Plant transformation binary vector
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Growth instructions: Please note: a binary vector-compatible Agrobacterium strain (such as GV3850) will have to be used prior to plant transformation. Plasmid should be grown at 30C after transformation to Agrobacterium (37C is ok for E. coli).
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: avrPto
• Alt name: avrPto1
• Alt name: avrPto1DC3000
• Species: Pseudomonas syringae pv. tomato DC3000
• Insert Size (bp): 495
• GenBank ID: NC_004578.1
• Promoter: dexamethasone inducible

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: CCGCTCGAGACCATGGGAAATATATGTGTC
• 3′ sequencing primer: GACTAGTTCATTGCCAGTTACGGTACG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

An efficient Agrobacterium-mediated transient transformation of Arabidopsis.
K. Tsuda et al. Plant J. 2012 Feb;69(4):713-9