PSL1180polyUBdsRED
(Plasmid #49327)

• Purpose: Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
• Depositing Lab: Leslie Vosshall
• Publication: Aedes homologous recombination donor plasmids (unpublished)

Backbone

• Vector backbone: psl1180
• Backbone size w/o insert (bp): 3422
• Total vector size (bp): 5574
• Modifications to backbone: Aedes polyubiquitin promoter and dsRED were cloned as a single fragment into psl1180 using BamH1 and Mlu1.
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dsRED
• Insert Size (bp): 681
• Promoter: Aedes polyubiquitin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Mlu1 (not destroyed)
• 5′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • psl1180polyUBdsRED genbank annotated sequence
• A portion of this plasmid was derived from a plasmid made by: Aedes aegypti PUb promoter and dsRED used in pSL1180polyUBdsRED was cloned using BamH1/Mlu1 from pMos-PUbDsRed-5HE-MCS-5HE as cited in Carpenetti et al. (2012) Insect Mol Biol. 21 : 97-106 (a kind gift of Dr Zach Adelman, Virginia Tech).
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's quality control sequencing finds a one nucleotide discrepancy in the Aedes aegypti PUb promoter. This discrepancy does not affect promoter function, and the plasmid should work as described.