PurposeDonor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49327||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3422
- Total vector size (bp) 5574
Modifications to backboneAedes polyubiquitin promoter and dsRED were cloned as a single fragment into psl1180 using BamH1 and Mlu1.
Vector typeInsect Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)681
- Promoter Aedes polyubiquitin
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site Mlu1 (not destroyed)
- 5′ sequencing primer M13 reverse (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byAedes aegypti PUb promoter and dsRED used in pSL1180polyUBdsRED was cloned using BamH1/Mlu1 from pMos-PUbDsRed-5HE-MCS-5HE as cited in Carpenetti et al. (2012) Insect Mol Biol. 21 : 97-106 (a kind gift of Dr Zach Adelman, Virginia Tech).
Terms and Licenses
Article Citing this Plasmid
Addgene's quality control sequencing finds a one nucleotide discrepancy in the Aedes aegypti PUb promoter. This discrepancy does not affect promoter function, and the plasmid should work as described.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PSL1180polyUBdsRED was a gift from Leslie Vosshall (Addgene plasmid # 49327 ; http://n2t.net/addgene:49327 ; RRID:Addgene_49327)