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pGL3 2198
(Plasmid #49347)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49347 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3 basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bmp2 promoter (shorter sequence)
  • Alt name
    pGL3 2185
  • Species
    H. sapiens (human)
  • Entrez Gene
    BMP2 (a.k.a. BDA2, BMP2A, SSFSC, SSFSC1)
  • Promoter Bmp2 promoter
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer NA
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3 2198 was a gift from Stephen Harris & Melissa Rogers (Addgene plasmid # 49347 ; http://n2t.net/addgene:49347 ; RRID:Addgene_49347)
  • For your References section:

    Bone morphogenetic protein 2 transcripts in rapidly developing deer antler tissue contain an extended 5' non-coding region arising from a distal promoter. Feng JQ, Chen D, Ghosh-Choudhury N, Esparza J, Mundy GR, Harris SE. Biochim Biophys Acta. 1997 Jan 3;1350(1):47-52. 10.1016/S0167-4781(96)00178-9 PubMed 9003457