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pMPRAdonor3
(Plasmid #49354)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49354 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 2693
  • Total vector size (bp) 4477
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    luc2
  • Alt name
    Firefly luciferase
  • Species
    Synthetic
  • Insert Size (bp)
    4663
  • Promoter SV40mod

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GTAAAACGACGGCCAGTG
  • 3′ sequencing primer GGAAACAGCTATGACCATG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthesized by Genscript
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SV40mod promoter is an SV40 early promoter modified to remove an internal SfiI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMPRAdonor3 was a gift from Tarjei Mikkelsen (Addgene plasmid # 49354 ; http://n2t.net/addgene:49354 ; RRID:Addgene_49354)
  • For your References section:

    Massively parallel reporter assays in cultured Mammalian cells. Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS. J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719. 10.3791/51719 PubMed 25177895