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pCFD4-U6:1_U6:3tandemgRNAs Citations (43)

Plasmid Article: Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.
Port F, Chen HM, Lee T, Bullock SL Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
PubMed Journal

Articles Citing pCFD4-U6:1_U6:3tandemgRNAs

Cas9-based genome editing in Drosophila. Housden BE, Lin S, Perrimon N. Methods Enzymol. 2014;546:415-39. doi: 10.1016/B978-0-12-801185-0.00019-2. PubMed
Drosophila Lgr3 Couples Organ Growth with Maturation and Ensures Developmental Stability. Colombani J, Andersen DS, Boulan L, Boone E, Romero N, Virolle V, Texada M, Leopold P. Curr Biol. 2015 Oct 19;25(20):2723-9. doi: 10.1016/j.cub.2015.09.020. Epub 2015 Oct 1. PubMed
The postsynaptic t-SNARE Syntaxin 4 controls traffic of Neuroligin 1 and Synaptotagmin 4 to regulate retrograde signaling. Harris KP, Zhang YV, Piccioli ZD, Perrimon N, Littleton JT. Elife. 2016 May 25;5. pii: e13881. doi: 10.7554/eLife.13881. PubMed
Editing Transgenic DNA Components by Inducible Gene Replacement in Drosophila melanogaster. Lin CC, Potter CJ. Genetics. 2016 Jun 22. pii: genetics.116.191783. PubMed

Associated Plasmids

Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Co-conversion. Ge DT, Tipping C, Brodsky MH, Zamore PD. G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 10.1534/g3.116.032557. PubMed

Associated Plasmids

Creating Heritable Mutations in Drosophila with CRISPR-Cas9. Port F, Bullock SL. Methods Mol Biol. 2016;1478:145-160. PubMed
The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression. Boone E, Colombani J, Andersen DS, Leopold P. Nat Commun. 2016 Nov 22;7:13505. doi: 10.1038/ncomms13505. PubMed
Peptidoglycan sensing by octopaminergic neurons modulates Drosophila oviposition. Kurz CL, Charroux B, Chaduli D, Viallat-Lieutaud A, Royet J. Elife. 2017 Mar 7;6. doi: 10.7554/eLife.21937. PubMed
Neuronal cell fate specification by the molecular convergence of different spatio-temporal cues on a common initiator terminal selector gene. Stratmann J, Thor S. PLoS Genet. 2017 Apr 17;13(4):e1006729. doi: 10.1371/journal.pgen.1006729. eCollection 2017 Apr. PubMed
SLC46 Family Transporters Facilitate Cytosolic Innate Immune Recognition of Monomeric Peptidoglycans. Paik D, Monahan A, Caffrey DR, Elling R, Goldman WE, Silverman N. J Immunol. 2017 Jul 1;199(1):263-270. doi: 10.4049/jimmunol.1600409. Epub 2017 May 24. PubMed
A Peptidergic Circuit Links the Circadian Clock to Locomotor Activity. King AN, Barber AF, Smith AE, Dreyer AP, Sitaraman D, Nitabach MN, Cavanaugh DJ, Sehgal A. Curr Biol. 2017 Jul 10;27(13):1915-1927.e5. doi: 10.1016/j.cub.2017.05.089. Epub 2017 Jun 29. PubMed
Multiple P450s and Variation in Neuronal Genes Underpins the Response to the Insecticide Imidacloprid in a Population of Drosophila melanogaster. Denecke S, Fusetto R, Martelli F, Giang A, Battlay P, Fournier-Level A, O' Hair RA, Batterham P. Sci Rep. 2017 Sep 12;7(1):11338. doi: 10.1038/s41598-017-11092-5. PubMed
Opbp is a new architectural/insulator protein required for ribosomal gene expression. Zolotarev N, Maksimenko O, Kyrchanova O, Sokolinskaya E, Osadchiy I, Girardot C, Bonchuk A, Ciglar L, Furlong EEM, Georgiev P. Nucleic Acids Res. 2017 Dec 1;45(21):12285-12300. doi: 10.1093/nar/gkx840. PubMed
Describing the role of Drosophila melanogaster ABC transporters in insecticide biology using CRISPR-Cas9 knockouts. Denecke S, Fusetto R, Batterham P. Insect Biochem Mol Biol. 2017 Dec;91:1-9. doi: 10.1016/j.ibmb.2017.09.017. Epub 2017 Oct 20. PubMed
CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity. Xu XS, Gantz VM, Siomava N, Bier E. Elife. 2017 Dec 23;6. pii: 30281. doi: 10.7554/eLife.30281. PubMed
Cytosolic and Secreted Peptidoglycan-Degrading Enzymes in Drosophila Respectively Control Local and Systemic Immune Responses to Microbiota. Charroux B, Capo F, Kurz CL, Peslier S, Chaduli D, Viallat-Lieutaud A, Royet J. Cell Host Microbe. 2018 Feb 14;23(2):215-228.e4. doi: 10.1016/j.chom.2017.12.007. Epub 2018 Feb 1. PubMed
A Conserved Circadian Function for the Neurofibromatosis 1 Gene. Bai L, Lee Y, Hsu CT, Williams JA, Cavanaugh D, Zheng X, Stein C, Haynes P, Wang H, Gutmann DH, Sehgal A. Cell Rep. 2018 Mar 27;22(13):3416-3426. doi: 10.1016/j.celrep.2018.03.014. PubMed
A population of adult satellite-like cells in Drosophila is maintained through a switch in RNA-isoforms. Boukhatmi H, Bray S. Elife. 2018 Apr 9;7. pii: 35954. doi: 10.7554/eLife.35954. PubMed
Reducing resistance allele formation in CRISPR gene drive. Champer J, Liu J, Oh SY, Reeves R, Luthra A, Oakes N, Clark AG, Messer PW. Proc Natl Acad Sci U S A. 2018 May 22;115(21):5522-5527. doi: 10.1073/pnas.1720354115. Epub 2018 May 7. PubMed
gamma-TuRC Heterogeneity Revealed by Analysis of Mozart1. Tovey CA, Tubman CE, Hamrud E, Zhu Z, Dyas AE, Butterfield AN, Fyfe A, Johnson E, Conduit PT. Curr Biol. 2018 Jul 23;28(14):2314-2323.e6. doi: 10.1016/j.cub.2018.05.044. Epub 2018 Jul 5. PubMed
A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling. Sandler JE, Irizarry J, Stepanik V, Dunipace L, Amrhein H, Stathopoulos A. Dev Cell. 2018 Dec 17;47(6):773-784.e6. doi: 10.1016/j.devcel.2018.11.019. PubMed
The apical protein Apnoia interacts with Crumbs to regulate tracheal growth and inflation. Skouloudaki K, Papadopoulos DK, Tomancak P, Knust E. PLoS Genet. 2019 Jan 15;15(1):e1007852. doi: 10.1371/journal.pgen.1007852. eCollection 2019 Jan. PubMed
Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm. Laurinyecz B, Vedelek V, Kovacs AL, Szilasi K, Lipinszki Z, Slezak C, Darula Z, Juhasz G, Sinka R. PLoS Genet. 2019 Feb 25;15(2):e1007987. doi: 10.1371/journal.pgen.1007987. eCollection 2019 Feb. PubMed
The molecular and cellular basis of olfactory response to tsetse fly attractants. Chahda JS, Soni N, Sun JS, Ebrahim SAM, Weiss BL, Carlson JR. PLoS Genet. 2019 Mar 15;15(3):e1008005. doi: 10.1371/journal.pgen.1008005. eCollection 2019 Mar. PubMed
A Cut/cohesin axis alters the chromatin landscape to facilitate neuroblast death. Arya R, Gyonjyan S, Harding K, Sarkissian T, Li Y, Zhou L, White K. Development. 2019 May 1;146(9). pii: dev.166603. doi: 10.1242/dev.166603. PubMed
Dual separable feedback systems govern firing rate homeostasis. Kulik Y, Jones R, Moughamian AJ, Whippen J, Davis GW. Elife. 2019 Apr 11;8. pii: 45717. doi: 10.7554/eLife.45717. PubMed
Comprehensive Genetic Characterization of Mitochondrial Ca(2+) Uniporter Components Reveals Their Different Physiological Requirements In Vivo. Tufi R, Gleeson TP, von Stockum S, Hewitt VL, Lee JJ, Terriente-Felix A, Sanchez-Martinez A, Ziviani E, Whitworth AJ. Cell Rep. 2019 Apr 30;27(5):1541-1550.e5. doi: 10.1016/j.celrep.2019.04.033. PubMed
Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila. Meltzer H, Marom E, Alyagor I, Mayseless O, Berkun V, Segal-Gilboa N, Unger T, Luginbuhl D, Schuldiner O. Nat Commun. 2019 May 8;10(1):2113. doi: 10.1038/s41467-019-10140-0. PubMed
Chemosensory sensilla of the Drosophila wing express a candidate ionotropic pheromone receptor. He Z, Luo Y, Shang X, Sun JS, Carlson JR. PLoS Biol. 2019 May 21;17(5):e2006619. doi: 10.1371/journal.pbio.2006619. eCollection 2019 May. PubMed
Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery. Munafo M, Manelli V, Falconio FA, Sawle A, Kneuss E, Eastwood EL, Seah JWE, Czech B, Hannon GJ. Genes Dev. 2019 Jul 1;33(13-14):844-856. doi: 10.1101/gad.325662.119. Epub 2019 May 23. PubMed
piRNA-guided co-transcriptional silencing coopts nuclear export factors. Fabry MH, Ciabrelli F, Munafo M, Eastwood EL, Kneuss E, Falciatori I, Falconio FA, Hannon GJ, Czech B. Elife. 2019 Jun 20;8. pii: 47999. doi: 10.7554/eLife.47999. PubMed
Anterior CNS expansion driven by brain transcription factors. Curt JR, Yaghmaeian Salmani B, Thor S. Elife. 2019 Jul 4;8. pii: 45274. doi: 10.7554/eLife.45274. PubMed
Parcas is the predominant Rab11-GEF for rhodopsin transport in Drosophila photoreceptors. Otsuka Y, Satoh T, Nakayama N, Inaba R, Yamashita H, Satoh AK. J Cell Sci. 2019 Aug 7;132(15). pii: jcs.231431. doi: 10.1242/jcs.231431. PubMed
Multilevel regulation of the glass locus during Drosophila eye development. Fritsch C, Bernardo-Garcia FJ, Humberg TH, Mishra AK, Miellet S, Almeida S, Frochaux MV, Deplancke B, Huber A, Sprecher SG. PLoS Genet. 2019 Jul 12;15(7):e1008269. doi: 10.1371/journal.pgen.1008269. eCollection 2019 Jul. PubMed
Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo. Pajak A, Laine I, Clemente P, El-Fissi N, Schober FA, Maffezzini C, Calvo-Garrido J, Wibom R, Filograna R, Dhir A, Wedell A, Freyer C, Wredenberg A. PLoS Genet. 2019 Jul 31;15(7):e1008240. doi: 10.1371/journal.pgen.1008240. eCollection 2019 Jul. PubMed
Functional Comparison of Short and Long Isoforms of the TRF2 Protein in Drosophila melanogaster. Osadchiy IS, Georgiev PG, Maksimenko OG. Dokl Biochem Biophys. 2019 May;486(1):224-228. doi: 10.1134/S1607672919030165. Epub 2019 Jul 31. PubMed
Specialization of the Drosophila nuclear export family protein Nxf3 for piRNA precursor export. Kneuss E, Munafo M, Eastwood EL, Deumer US, Preall JB, Hannon GJ, Czech B. Genes Dev. 2019 Sep 1;33(17-18):1208-1220. doi: 10.1101/gad.328690.119. Epub 2019 Aug 15. PubMed
CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila. Fritsch C, Sprecher SG. Methods Mol Biol. 2020;2047:161-189. doi: 10.1007/978-1-4939-9732-9_10. PubMed
Drosulfakinin signaling in fruitless circuitry antagonizes P1 neurons to regulate sexual arousal in Drosophila. Wu S, Guo C, Zhao H, Sun M, Chen J, Han C, Peng Q, Qiao H, Peng P, Liu Y, Luo SD, Pan Y. Nat Commun. 2019 Oct 18;10(1):4770. doi: 10.1038/s41467-019-12758-6. PubMed
Robust olfactory responses in the absence of odorant binding proteins. Xiao S, Sun JS, Carlson JR. Elife. 2019 Oct 25;8. pii: 51040. doi: 10.7554/eLife.51040. PubMed
Dopamine modulates the optomotor response to unreliable visual stimuli in Drosophila melanogaster. Akiba M, Sugimoto K, Aoki R, Murakami R, Miyashita T, Hashimoto R, Hiranuma A, Yamauchi J, Ueno T, Morimoto T. Eur J Neurosci. 2020 Feb;51(3):822-839. doi: 10.1111/ejn.14648. Epub 2019 Dec 30. PubMed
Molecular Logic and Evolution of Bitter Taste in Drosophila. Dweck HKM, Carlson JR. Curr Biol. 2020 Jan 6;30(1):17-30.e3. doi: 10.1016/j.cub.2019.11.005. Epub 2019 Dec 12. PubMed
Evolutionary trade-offs of insecticide resistance - The fitness costs associated with target-site mutations in the nAChR of Drosophila melanogaster. Homem RA, Buttery B, Richardson E, Tan Y, Field LM, Williamson MS, Emyr Davies TG. Mol Ecol. 2020 Jul;29(14):2661-2675. doi: 10.1111/mec.15503. Epub 2020 Jun 22. PubMed

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