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Matrix-roGFP
(Plasmid #49437)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49437 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    VQ Ad5CMV K-NpA
  • Backbone manufacturer
    ViraQuest Inc.
  • Backbone size w/o insert (bp) 6217
  • Total vector size (bp) 6970
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Matrix-roGFP2
  • Alt name
    Mitochondrial roGFP2
  • Species
    Synthetic
  • Insert Size (bp)
    780
  • Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's GFP
  • Promoter CMV
  • Tag / Fusion Protein
    • Cytochrome c oxidase subunit IV mitochondrial targeting sequence (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer 5'GGCGTGTACGGTGGGAGGTCTATA-3'
  • 3′ sequencing primer 5'-TTAAAAAACCTCCCCACCTCCCCC-3'
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We inserted mutations into Clontech's eGFP gene to convert it into roGFP and cloned by PCR using a primer to insert the mitochondrial targeting sequence for cyt c oxidase subunit IV 5' to the roGFP2. This entire construct was then ligated into the VQ vector between KpnI and NotI sites.
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Matrix-roGFP was a gift from Paul Schumacker (Addgene plasmid # 49437 ; http://n2t.net/addgene:49437 ; RRID:Addgene_49437)
  • For your References section:

    Hypoxia triggers subcellular compartmental redox signaling in vascular smooth muscle cells. Waypa GB, Marks JD, Guzy R, Mungai PT, Schriewer J, Dokic D, Schumacker PT. Circ Res. 2010 Feb 19;106(3):526-35. doi: 10.1161/CIRCRESAHA.109.206334. Epub 2009 Dec 17. 10.1161/CIRCRESAHA.109.206334 PubMed 20019331