pRKO
(Plasmid
#49456)
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PurposeloxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 49456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRX1
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Backbone manufacturerYu X. and Gabriel,A. (1999)
- Total vector size (bp) 8000
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Vector typeBacterial Expression, Cre/Lox
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameloxP-kanMX-loxP cassette
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SpeciesSynthetic
- Promoter none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer M13_forward20_primer
- 3′ sequencing primer M13_Reverse_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRKO was a gift from Marc Gartenberg (Addgene plasmid # 49456 ; http://n2t.net/addgene:49456 ; RRID:Addgene_49456) -
For your References section:
Controlling gene expression in yeast by inducible site-specific recombination. Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR. Nucleic Acids Res. 2000 Dec 15;28(24):E108. 10.1093/nar/28.24.e108 PubMed 11121495
