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lentiCRISPR Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme(s): NheI and an NheI/NotI double digest.

We expected the following band sizes for this digest: 13.5kb with NheI alone (linearization); 7.7kb and 5.7kb bands with NheI and NotI. We see the expected pattern.