pcDNA3.1(+)-wt-TTF-1
(Plasmid #49989)

• Purpose: For transient transfection to express human wild type TTF-1 protein
• Depositing Lab: David Mu
• Publication: Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-801. doi: 10.1074/jbc.M112.367987. Epub 2012 Jul 2.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6516
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TTF-1
• Alt name: NKX2-1
• Alt name: TITF1
• Species: H. sapiens (human)
• Insert Size (bp): 1116
• GenBank ID: NM_003317
• Entrez Gene: NKX2-1 (a.k.a. BCH, BHC, NK-2, NKX2.1, NKX2A, NMTC1, T/EBP, TEBP, TITF1, TTF-1, TTF1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry