pBS-Int
(Plasmid #50000)

• Purpose: Expresses the integrase on a suicide plasmid
• Depositing Labs: Brian Robertson, Peter Sander
• Publication: Springer et al Int J Med Microbiol. 2001 Mar;290(8):669-75.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 4850
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Integrase from pMV361
• Alt name: int
• Species: Mycobcterium
• Insert Size (bp): 1850
• Promoter: own

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13/T7/T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was constructed in the laboratory of Professor Erik C. Bottger and Dr. Peter Sander, who have kindly agreed that we submit this plasmid to Addgene on their behalf.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry