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pCMV3flag8SHARPIN
(Plasmid #50014)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50014 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-3Tag-8
  • Backbone manufacturer
    Agilent (Stratagene)
  • Backbone size w/o insert (bp) 5171
  • Total vector size (bp) 6339
  • Modifications to backbone
    Replaced with new MCS: XhoI-Insert[ACCGGAG(5'UT)-Gene CDS(1164bp no Stop)]-NotI-Cla - 3 x FLAG
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHANK-associated RH domain interactor
  • Alt name
    Sharpin
  • Alt name
    SIPL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1168
  • GenBank ID
  • Entrez Gene
    SHARPIN (a.k.a. SIPL1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3 x FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMVforward
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DF/HCC DNA Resource Core, 240 Longwood Avenue Seeley Mudd 228 Boston, MA 02115 (617) 432-7708 Plasmid ID HsCD00332474
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV3flag8SHARPIN was a gift from Martin Dorf (Addgene plasmid # 50014 ; http://n2t.net/addgene:50014 ; RRID:Addgene_50014)
  • For your References section:

    The ubiquitin conjugating enzyme UBE2L3 regulates TNFalpha-induced linear ubiquitination. Fu B, Li S, Wang L, Berman MA, Dorf ME. Cell Res. 2013 Sep 24. doi: 10.1038/cr.2013.133. 10.1038/cr.2013.133 PubMed 24060851