Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pLYS1-MICU1-Flag
(Plasmid #50058)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50058 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLYS1
  • Backbone size w/o insert (bp) 7393
  • Total vector size (bp) 8824
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MICU1 (isoform 2)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1431
  • Mutation
    *
  • GenBank ID
    NM_001195518
  • Entrez Gene
    MICU1 (a.k.a. CALC, CBARA1, EFHA3, MPXPS, ara CALC)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Xma1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer custom
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*There are some silent mutations at the 3' end of the insert. These were used for resistance to a specific shRNA and will not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLYS1-MICU1-Flag was a gift from Vamsi Mootha (Addgene plasmid # 50058 ; http://n2t.net/addgene:50058 ; RRID:Addgene_50058)
  • For your References section:

    EMRE is an Essential Component of the Mitochondrial Calcium Uniporter Complex. Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O, Mootha VK. Science. 2013 Nov 14. 10.1126/science.1242993 PubMed 24231807