pAFW-Ago2
(Plasmid #50554)

• Purpose: Expression of FLAG tagged Ago2 in S2 cell
• Depositing Lab: Yukihide Tomari
• Publication: Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi: 10.1016/j.molcel.2010.05.015. Epub 2010 Jun 3.

Backbone

• Vector backbone: pAFW
• Backbone manufacturer: The Drosophila Gateway™ Vector Collection
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Argonaute2
• Species: D. melanogaster (fly)
• Entrez Gene: AGO2 (a.k.a. Dmel_CG7439, AG02, AGO 2, AGO1, Ago-2, Ago2, Ago2a, CG13452, CG7439, Dm Ago2, DmAgo2, Dmel\CG7439, ago, ago-2, ago2, argonaute2, dAGO2, dAgo2, eIF2C2)
• Promoter: Actin5C
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: AC5

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene origin is from Phillip Zamore at Univ. of Massachusetts.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that when compared to GenBank reference sequence NP_648775.1, the Ago2 sequence in this plasmid is missing amino acids 49-54 (LQQPQQ).