Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pHIS3p:mRuby2-Tub1+3'UTR::URA3
(Plasmid #50639)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50639 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRuby2-Tub1
  • Alt name
    mRuby2-a-tubulin
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2177
  • Promoter HIS3p
  • Tag / Fusion Protein
    • mRuby2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIS3p:mRuby2-Tub1+3'UTR::URA3 was a gift from Wei-Lih Lee (Addgene plasmid # 50639 ; http://n2t.net/addgene:50639 ; RRID:Addgene_50639)
  • For your References section:

    Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae. Markus SM, Omer S, Baranowski K, Lee WL. Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 Apr 28. 10.1111/tra.12276 PubMed 25711127