pMo130-TelR
(Plasmid #50799)

• Purpose: Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria
• Depositing Lab: Kim Lee Chua
• Publication: Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi: 10.1186/1471-2180-13-158.

Backbone

• Vector backbone: pMo130
• Backbone manufacturer: Martin Voskuil
• Backbone size w/o insert (bp): 6127
• Total vector size (bp): 9392
• Vector type: Suicide vector
• Selectable markers: Tellurite

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Also resistant to Tellurite
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tellurite-resistance cassette
• Alt name: TelR
• Species: RK2 plasmid
• Insert Size (bp): 3266
• Promoter: PCs12

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: 5'-CGGGTACCGAGCTCGAATTA-3'
• 3′ sequencing primer: 5'-CCGGGTACCGAGCTCGAATT-3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gift from Dr Tung T. Hoang, Department of Microbiology, University of Hawaii at Manoa, 2358 The Mall-Snyder 310, Honolulu HI 96822
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry