pLKO.1-puro U6 sgRNA BfuAI stuffer Citations (17)
Originally described in: Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. PubMed Journal
Articles Citing pLKO.1-puro U6 sgRNA BfuAI stuffer
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| CRISPR/dCas9-mediated Transcriptional Inhibition Ameliorates the Epigenetic Dysregulation at D4Z4 and Represses DUX4-fl in FSH Muscular Dystrophy. Himeda CL, Jones TI, Jones PL. Mol Ther. 2015 Nov 3. doi: 10.1038/mt.2015.200. PubMed |
| Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations. Maggio I, Stefanucci L, Janssen JM, Liu J, Chen X, Mouly V, Goncalves MA. Nucleic Acids Res. 2016 Jan 13. pii: gkv1540. PubMed |
Probing the impact of chromatin conformation on genome editing tools.
Chen X, Rinsma M, Janssen JM, Liu J, Maggio I, Goncalves MA.
Nucleic Acids Res. 2016 Jun 8. pii: gkw524.
PubMed
Associated Plasmids |
| A novel isoform of TET1 that lacks a CXXC domain is overexpressed in cancer. Good CR, Madzo J, Patel B, Maegawa S, Engel N, Jelinek J, Issa JJ. Nucleic Acids Res. 2017 May 22. doi: 10.1093/nar/gkx435. PubMed |
| The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation. Khan MR, Xiang S, Song Z, Wu M. EMBO J. 2017 Dec 1;36(23):3483-3500. doi: 10.15252/embj.201696239. Epub 2017 Oct 18. PubMed |
| Targeting ectodysplasin promotor by CRISPR/dCas9-effector effectively induces the reprogramming of human bone marrow-derived mesenchymal stem cells into sweat gland-like cells. Sun S, Xiao J, Huo J, Geng Z, Ma K, Sun X, Fu X. Stem Cell Res Ther. 2018 Jan 12;9(1):8. doi: 10.1186/s13287-017-0758-0. PubMed |
| Knockdown of Human AMPK Using the CRISPR/Cas9 Genome-Editing System. Grenier A, Sujobert P, Olivier S, Guermouche H, Mondesir J, Kosmider O, Viollet B, Tamburini J. Methods Mol Biol. 2018;1732:171-194. doi: 10.1007/978-1-4939-7598-3_11. PubMed |
| Identification of Epigenetic Regulators of DUX4-fl for Targeted Therapy of Facioscapulohumeral Muscular Dystrophy. Himeda CL, Jones TI, Virbasius CM, Zhu LJ, Green MR, Jones PL. Mol Ther. 2018 Jul 5;26(7):1797-1807. doi: 10.1016/j.ymthe.2018.04.019. Epub 2018 Apr 26. PubMed |
| Quantitative proteomics analysis identifies MUC1 as an effect sensor of EGFR inhibition. de Boer HR, Pool M, Joosten E, Everts M, Samplonius DF, Helfrich W, Groen HJM, van Cooten S, Fusetti F, Fehrmann RSN, de Vries EGE, van Vugt MATM. Oncogene. 2019 Feb;38(9):1477-1488. doi: 10.1038/s41388-018-0522-7. Epub 2018 Oct 10. PubMed |
| SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Yang H, Shen H, Li J, Guo LW. Autophagy. 2019 Sep;15(9):1539-1557. doi: 10.1080/15548627.2019.1586248. Epub 2019 Mar 14. PubMed |
| Bridge Helix of Cas9 Modulates Target DNA Cleavage and Mismatch Tolerance. Babu K, Amrani N, Jiang W, Yogesha SD, Nguyen R, Qin PZ, Rajan R. Biochemistry. 2019 Apr 9;58(14):1905-1917. doi: 10.1021/acs.biochem.8b01241. Epub 2019 Mar 27. PubMed |
G-tract RNA removes Polycomb repressive complex 2 from genes.
Beltran M, Tavares M, Justin N, Khandelwal G, Ambrose J, Foster BM, Worlock KB, Tvardovskiy A, Kunzelmann S, Herrero J, Bartke T, Gamblin SJ, Wilson JR, Jenner RG.
Nat Struct Mol Biol. 2019 Oct;26(10):899-909. doi: 10.1038/s41594-019-0293-z. Epub 2019 Sep 23.
PubMed
Associated Plasmids |
| LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells. LeRoy G, Oksuz O, Descostes N, Aoi Y, Ganai RA, Kara HO, Yu JR, Lee CH, Stafford J, Shilatifard A, Reinberg D. Sci Adv. 2019 Oct 2;5(10):eaay3068. doi: 10.1126/sciadv.aay3068. eCollection 2019 Oct. PubMed |
| TGF-beta-Induced Endothelial to Mesenchymal Transition Is Determined by a Balance Between SNAIL and ID Factors. Ma J, van der Zon G, Goncalves MAFV, van Dinther M, Thorikay M, Sanchez-Duffhues G, Ten Dijke P. Front Cell Dev Biol. 2021 Feb 12;9:616610. doi: 10.3389/fcell.2021.616610. eCollection 2021. PubMed |
| A cis-acting mechanism mediates transcriptional memory at Polycomb target genes in mammals. Holoch D, Wassef M, Lovkvist C, Zielinski D, Aflaki S, Lombard B, Hery T, Loew D, Howard M, Margueron R. Nat Genet. 2021 Dec;53(12):1686-1697. doi: 10.1038/s41588-021-00964-2. Epub 2021 Nov 15. PubMed |
| A protocol for CRISPR-mediated activation and repression of human endogenous retroviruses in human pluripotent stem cells. Nair VP, Mayer J, Vincendeau M. STAR Protoc. 2022 Apr 12;3(2):101281. doi: 10.1016/j.xpro.2022.101281. eCollection 2022 Jun 17. PubMed |
| Vitamin C epigenetically controls osteogenesis and bone mineralization. Thaler R, Khani F, Sturmlechner I, Dehghani SS, Denbeigh JM, Zhou X, Pichurin O, Dudakovic A, Jerez SS, Zhong J, Lee JH, Natarajan R, Kalajzic I, Jiang YH, Deyle DR, Paschalis EP, Misof BM, Ordog T, van Wijnen AJ. Nat Commun. 2022 Oct 6;13(1):5883. doi: 10.1038/s41467-022-32915-8. PubMed |
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