Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pSLQ1658-dCas9-EGFP Citations (14)

Originally described in: Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.
Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
PubMed Journal

Articles Citing pSLQ1658-dCas9-EGFP

Articles
CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci. Fu Y, Rocha PP, Luo VM, Raviram R, Deng Y, Mazzoni EO, Skok JA. Nat Commun. 2016 May 25;7:11707. doi: 10.1038/ncomms11707. PubMed
CRISPR-dCas9 mediated TET1 targeting for selective DNA demethylation at BRCA1 promoter. Choudhury SR, Cui Y, Lubecka K, Stefanska B, Irudayaraj J. Oncotarget. 2016 Jun 23. doi: 10.18632/oncotarget.10234. PubMed
(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Neguembor MV, Sebastian-Perez R, Aulicino F, Gomez-Garcia PA, Cosma MP, Lakadamyali M. Nucleic Acids Res. 2018 Mar 16;46(5):e30. doi: 10.1093/nar/gkx1271. PubMed
Transcriptional Dysregulation of MYC Reveals Common Enhancer-Docking Mechanism. Schuijers J, Manteiga JC, Weintraub AS, Day DS, Zamudio AV, Hnisz D, Lee TI, Young RA. Cell Rep. 2018 Apr 10;23(2):349-360. doi: 10.1016/j.celrep.2018.03.056. PubMed
Dynamics of sister chromatid resolution during cell cycle progression. Stanyte R, Nuebler J, Blaukopf C, Hoefler R, Stocsits R, Peters JM, Gerlich DW. J Cell Biol. 2018 Jun 4;217(6):1985-2004. doi: 10.1083/jcb.201801157. Epub 2018 Apr 25. PubMed
A CRISPR/molecular beacon hybrid system for live-cell genomic imaging. Wu X, Mao S, Yang Y, Rushdi MN, Krueger CJ, Chen AK. Nucleic Acids Res. 2018 Jul 27;46(13):e80. doi: 10.1093/nar/gky304. PubMed

Associated Plasmids

Interstitial telomeric loops and implications of the interaction between TRF2 and lamin A/C. Smith ED, Garza-Gongora AG, MacQuarrie KL, Kosak ST. Differentiation. 2018 Jul - Aug;102:19-26. doi: 10.1016/j.diff.2018.06.001. Epub 2018 Jun 18. PubMed
CRISPR-based genomic loci labeling revealed ordered spatial organization of chromatin in living diploid human cells. Guo DG, Wang DB, Liu C, Lu S, Hou Y, Zhang XE. Biochim Biophys Acta Mol Cell Res. 2019 Dec;1866(12):118518. doi: 10.1016/j.bbamcr.2019.07.013. Epub 2019 Jul 31. PubMed
CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci. Mao S, Ying Y, Wu X, Krueger CJ, Chen AK. Nucleic Acids Res. 2019 Nov 18;47(20):e131. doi: 10.1093/nar/gkz752. PubMed

Associated Plasmids

Nuclear localization of Desmoplakin and its involvement in telomere maintenance. Li P, Meng Y, Wang Y, Li J, Lam M, Wang L, Di LJ. Int J Biol Sci. 2019 Aug 24;15(11):2350-2362. doi: 10.7150/ijbs.34450. eCollection 2019. PubMed
Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore. Chaudhary N, Nho SH, Cho H, Gantumur N, Ra JS, Myung K, Kim H. Genome Res. 2020 Sep;30(9):1306-1316. doi: 10.1101/gr.260018.119. Epub 2020 Sep 4. PubMed

Associated Plasmids

Illuminating single genomic loci in live cells by reducing nuclear background fluorescence. Lu S, Wang D, Hou Y, Guo D, Deng Y, Zhang XE. Sci China Life Sci. 2021 May;64(5):667-677. doi: 10.1007/s11427-020-1794-2. Epub 2020 Oct 26. PubMed
RNA transcripts stimulate homologous recombination by forming DR-loops. Ouyang J, Yadav T, Zhang JM, Yang H, Rheinbay E, Guo H, Haber DA, Lan L, Zou L. Nature. 2021 Jun;594(7862):283-288. doi: 10.1038/s41586-021-03538-8. Epub 2021 May 12. PubMed
Optogenetic Control of Background Fluorescence Reduction for CRISPR-Based Genome Imaging. Hou Y, Wang D, Lu S, Guo D, Li M, Cui M, Zhang XE. Anal Chem. 2022 Jun 21;94(24):8724-8731. doi: 10.1021/acs.analchem.2c01113. Epub 2022 Jun 6. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.