pUC57-sgRNA expression vector
Purpose(Empty Backbone) For in vitro transcription of sgRNA from the T7 promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51132||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namesgRNA scaffold
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (not destroyed)
- 3′ cloning site BsaI (not destroyed)
- 5′ sequencing primer pBAC
- 3′ sequencing primer Lac-promoter (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC57-sgRNA expression vector was a gift from Xingxu Huang (Addgene plasmid # 51132 ; http://n2t.net/addgene:51132 ; RRID:Addgene_51132)
For your References section:Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC. Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857. 10.1038/nmeth.2857 PubMed 24584192